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. 1986 Jun;166(3):978–984. doi: 10.1128/jb.166.3.978-984.1986

Structure of a conjugative element in Streptococcus pneumoniae.

M N Vijayakumar, S D Priebe, W R Guild
PMCID: PMC215221  PMID: 3011757

Abstract

We have cloned and mapped a 69-kilobase (kb) region of the chromosome of Streptococcus pneumoniae DP1322, which carries the conjugative omega (cat-tet) insertion from S. pneumoniae BM6001. This element proved to be 65.5 kb in size. Location of the junctions was facilitated by cloning a preferred target region from the wild-type strain Rx1 recipient genome. This target site was preferred by both the BM6001 element and the cat-erm-tet element from Streptococcus agalactiae B109. Within the BM6001 element cat and tet were separated by 30 kb, and cat was flanked by two copies of a sequence that was also present in the recipient strain Rx1 DNA. Another sequence at least 2.4 kb in size was found inside the BM6001 element and at two places in the Rx1 genome. Its role is unknown. The ends of the BM6001 element appear to be the same as those of the B109 element, both as seen after transfer to S. pneumoniae and as mapped by others in pDP5 after transposition in Streptococcus faecalis. We see no homology between the ends of the BM6001 element and find no evidence suggesting that it ever circularizes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernheimer H. P. Lysogenic pneumococci and their bacteriophages. J Bacteriol. 1979 May;138(2):618–624. doi: 10.1128/jb.138.2.618-624.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buu-Hoï A., Horodniceanu T. Conjugative transfer of multiple antibiotic resistance markers in Streptococcus pneumoniae. J Bacteriol. 1980 Jul;143(1):313–320. doi: 10.1128/jb.143.1.313-320.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clewell D. B. Plasmids, drug resistance, and gene transfer in the genus Streptococcus. Microbiol Rev. 1981 Sep;45(3):409–436. doi: 10.1128/mr.45.3.409-436.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Franke A. E., Clewell D. B. Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of "conjugal" transfer in the absence of a conjugative plasmid. J Bacteriol. 1981 Jan;145(1):494–502. doi: 10.1128/jb.145.1.494-502.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guild W. R., Shoemaker N. B. Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency. J Bacteriol. 1976 Jan;125(1):125–135. doi: 10.1128/jb.125.1.125-135.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  7. Horodniceanu T., Bougueleret L., Bieth G. Conjugative transfer of multiple-antibiotic resistance markers in beta-hemolytic group A, B, F, and G streptococci in the absence of extrachromosomal deoxyribonucleic acid. Plasmid. 1981 Mar;5(2):127–137. doi: 10.1016/0147-619x(81)90014-7. [DOI] [PubMed] [Google Scholar]
  8. Inamine J. M., Burdett V. Structural organization of a 67-kilobase streptococcal conjugative element mediating multiple antibiotic resistance. J Bacteriol. 1985 Feb;161(2):620–626. doi: 10.1128/jb.161.2.620-626.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mannarelli B. M., Lacks S. A. Ectopic integration of chromosomal genes in Streptococcus pneumoniae. J Bacteriol. 1984 Dec;160(3):867–873. doi: 10.1128/jb.160.3.867-873.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Méjean V., Claverys J. P., Vasseghi H., Sicard A. M. Rapid cloning of specific DNA fragments of Streptococcus pneumoniae by vector integration into the chromosome followed by endonucleolytic excision. Gene. 1981 Nov;15(2-3):289–293. doi: 10.1016/0378-1119(81)90139-6. [DOI] [PubMed] [Google Scholar]
  11. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  12. Sanger F., Coulson A. R., Hong G. F., Hill D. F., Petersen G. B. Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol. 1982 Dec 25;162(4):729–773. doi: 10.1016/0022-2836(82)90546-0. [DOI] [PubMed] [Google Scholar]
  13. Shoemaker N. B., Guild W. R. Destruction of low efficiency markers is a slow process occurring at a heteroduplex stage of transformation. Mol Gen Genet. 1974;128(4):283–290. doi: 10.1007/BF00268516. [DOI] [PubMed] [Google Scholar]
  14. Shoemaker N. B., Smith M. D., Guild W. R. DNase-resistant transfer of chromosomal cat and tet insertions by filter mating in Pneumococcus. Plasmid. 1980 Jan;3(1):80–87. doi: 10.1016/s0147-619x(80)90036-0. [DOI] [PubMed] [Google Scholar]
  15. Shoemaker N. B., Smith M. D., Guild W. R. Organization and transfer of heterologous chloramphenicol and tetracycline resistance genes in pneumococcus. J Bacteriol. 1979 Aug;139(2):432–441. doi: 10.1128/jb.139.2.432-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith M. D., Guild W. R. A plasmid in Streptococcus pneumoniae. J Bacteriol. 1979 Feb;137(2):735–739. doi: 10.1128/jb.137.2.735-739.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith M. D., Hazum S., Guild W. R. Homology among tet determinants in conjugative elements of streptococci. J Bacteriol. 1981 Oct;148(1):232–240. doi: 10.1128/jb.148.1.232-240.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  19. Vijayakumar M. N., Priebe S. D., Pozzi G., Hageman J. M., Guild W. R. Cloning and physical characterization of chromosomal conjugative elements in streptococci. J Bacteriol. 1986 Jun;166(3):972–977. doi: 10.1128/jb.166.3.972-977.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]

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