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. 1986 Jun;166(3):1089–1095. doi: 10.1128/jb.166.3.1089-1095.1986

camR, a negative regulator locus of the cytochrome P-450cam hydroxylase operon.

H Koga, H Aramaki, E Yamaguchi, K Takeuchi, T Horiuchi, I C Gunsalus
PMCID: PMC215236  PMID: 3011733

Abstract

A 4.27-kilobase insert from a HindIII DNA library of Pseudomonas putida carrying the CAM plasmid allowed coordinate expression of genes camD and camC under control of camR, an upstream regulator. The camC gene specifies cytochrome P-450cam, and camD specifies the 5-exo-alcohol dehydrogenase. A 1.38-kilobase deletion from the insert results in the constitutive expression of genes camC and camD; transformation in trans restores the substrate control, indicating that camR is a negative regulator.

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Selected References

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