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. 1986 Jun;166(3):1118–1122. doi: 10.1128/jb.166.3.1118-1122.1986

Molecular cloning, DNA nucleotide sequencing, and expression in Bacillus subtilis cells of the Bacillus macerans cyclodextrin glucanotransferase gene.

T Takano, M Fukuda, M Monma, S Kobayashi, K Kainuma, K Yamane
PMCID: PMC215240  PMID: 3011735

Abstract

The gene for cyclodextrin glucanotransferase from Bacillus macerans was cloned in an Escherichia coli bacteriophage, lambda D69, and was recloned in a Bacillus subtilis plasmid, pUB110. Starting from an ATG initiation codon, a unique reading frame was shown to extend for 2,142 base pairs (714 amino acids). The nucleotide sequence revealed that the enzyme is composed of two identical subunits.

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Selected References

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