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. 1986 Jun;166(3):1134–1136. doi: 10.1128/jb.166.3.1134-1136.1986

IS222, a new insertion element associated with the genome of Pseudomonas aeruginosa.

E Gertman, B N White, D Berry, A M Kropinski
PMCID: PMC215244  PMID: 3011736

Abstract

A new insertion element, IS222, was identified to be associated with the DNA of a mutant strain of the converting Pseudomonas aeruginosa bacteriophage D3. The insertion sequence was 1,350 base pairs in size and possessed terminal inverted repeats. The nucleotide sequence contained single cleavage sites for EcoRI and PvuI but none for BamHI, PstI, HindIII, SmaI, or SalI. By Southern hybridization analysis, no homology was found with genomic DNA from P. aeruginosa PAT or Escherichia coli. Genomic DNA from the phage host, P. aeruginosa PAO, contained two sequences homologous to IS222.

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Selected References

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