Abstract
The patterns of septation in filaments of Escherichia coli, formed as a consequence of the lack of an active ftsA gene product and then returned to permissive conditions, were analyzed in isogenic strains containing three different mutated alleles of ftsA. Septation was blocked for at least one doubling time at the potential septation sites that presumably contained inactive FtsA protein but not at those sites containing either the active gene product or no gene product at all. These results suggested a possible structural role for the ftsA gene product in the construction of the E. coli septum.
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- Begg K. J., Hatfull G. F., Donachie W. D. Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell division gene ftsQ. J Bacteriol. 1980 Oct;144(1):435–437. doi: 10.1128/jb.144.1.435-437.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Celis J. E., Hooper M. L., Smith J. D. Amino acid acceptor stem of E. coli suppressor tRNA tyr is a site of synthetase recognition. Nat New Biol. 1973 Aug 29;244(139):261–264. doi: 10.1038/newbio244261a0. [DOI] [PubMed] [Google Scholar]
- Donachie W. D., Begg K. J. Growth of the bacterial cell. Nature. 1970 Sep 19;227(5264):1220–1224. doi: 10.1038/2271220a0. [DOI] [PubMed] [Google Scholar]
- Donachie W. D., Begg K. J., Lutkenhaus J. F., Salmond G. P., Martinez-Salas E., Vincente M. Role of the ftsA gene product in control of Escherichia coli cell division. J Bacteriol. 1979 Nov;140(2):388–394. doi: 10.1128/jb.140.2.388-394.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Donachie W. D., Begg K. J., Vicente M. Cell length, cell growth and cell division. Nature. 1976 Nov 25;264(5584):328–333. doi: 10.1038/264328a0. [DOI] [PubMed] [Google Scholar]
- Donachie W. D. Relationship between cell size and time of initiation of DNA replication. Nature. 1968 Sep 7;219(5158):1077–1079. doi: 10.1038/2191077a0. [DOI] [PubMed] [Google Scholar]
- Fletcher G., Irwin C. A., Henson J. M., Fillingim C., Malone M. M., Walker J. R. Identification of the Escherichia coli cell division gene sep and organization of the cell division-cell envelope genes in the sep-mur-ftsA-envA cluster as determined with specialized transducing lambda bacteriophages. J Bacteriol. 1978 Jan;133(1):91–100. doi: 10.1128/jb.133.1.91-100.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones N. C., Donachie W. D. Chromosome replication, transcription and control of cell division in Escherichia coli. Nat New Biol. 1973 May 23;243(125):100–103. [PubMed] [Google Scholar]
- Lutkenhaus J. F., Donachie W. D. Identification of the ftsA gene product. J Bacteriol. 1979 Mar;137(3):1088–1094. doi: 10.1128/jb.137.3.1088-1094.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lutkenhaus J. F., Wolf-Watz H., Donachie W. D. Organization of genes in the ftsA-envA region of the Escherichia coli genetic map and identification of a new fts locus (ftsZ). J Bacteriol. 1980 May;142(2):615–620. doi: 10.1128/jb.142.2.615-620.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martínez-Salas E., Vicente M. Amber mutation affecting the length of Escherichia coli cells. J Bacteriol. 1980 Nov;144(2):532–541. doi: 10.1128/jb.144.2.532-541.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oliver D. B., Beckwith J. Identification of a new gene (secA) and gene product involved in the secretion of envelope proteins in Escherichia coli. J Bacteriol. 1982 May;150(2):686–691. doi: 10.1128/jb.150.2.686-691.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenberger R. F., Grover N. B., Zaritsky A., Woldringh C. L. Surface growth in rod-shaped bacteria. J Theor Biol. 1978 Aug 21;73(4):711–721. doi: 10.1016/0022-5193(78)90132-7. [DOI] [PubMed] [Google Scholar]
- Salmond G. P., Lutkenhaus J. F., Donachie W. D. Identification of new genes in a cell envelope-cell division gene cluster of Escherichia coli: cell envelope gene murG. J Bacteriol. 1980 Oct;144(1):438–440. doi: 10.1128/jb.144.1.438-440.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spratt B. G. Temperature-sensitive cell division mutants of Escherichia coli with thermolabile penicillin-binding proteins. J Bacteriol. 1977 Jul;131(1):293–305. doi: 10.1128/jb.131.1.293-305.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tormo A., Martínez-Salas E., Vicente M. Involvement of the ftsA gene product in late stages of the Escherichia coli cell cycle. J Bacteriol. 1980 Feb;141(2):806–813. doi: 10.1128/jb.141.2.806-813.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]