Abstract
In matings between Escherichia coli 2492(pJB4JI) and Erwinia carotovora subsp. carotovora Ecc71 and E. carotovora subsp. atroseptica Eca12, Kmr Gms transconjugants were obtained at high frequencies, indicating instability of the Mu-containing plasmid pJB4JI and transposition of Tn5 into the recipient genome. This was verified by Southern blot hybridization with pRZ102 DNA containing Tn5 as the 32P-labeled probe. Examination of Kmr Gms transconjugants of Ecc71 and Eca12 disclosed that a proportion (2 to 3%) were either auxotrophic or defective in catabolism of specific carbohydrates. Spontaneous prototrophic revertants were obtained for all markers with the exception of ilv, tyr, and suc. Genetic and physical data indicate that scattered insertions of Tn5 from pJb4JI into the chromosome of Ecc71 and Eca12 produced a variety of altered phenotypes due mostly to single insertions of Tn5 not accompanied by Mu DNA.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Calos M. P., Miller J. H. Transposable elements. Cell. 1980 Jul;20(3):579–595. doi: 10.1016/0092-8674(80)90305-0. [DOI] [PubMed] [Google Scholar]
- Chatterjee A. K. Acceptance by Erwinia spp. of R plasmid R68.45 and its ability to mobilize the chromosome of Erwinia chrysanthemi. J Bacteriol. 1980 Apr;142(1):111–119. doi: 10.1128/jb.142.1.111-119.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chatterjee A. K., Thurn K. K., Feese D. A. Tn5-Induced Mutations in the Enterobacterial Phytopathogen Erwinia chrysanthemi. Appl Environ Microbiol. 1983 Feb;45(2):644–650. doi: 10.1128/aem.45.2.644-650.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Comai L., Kosuge T. Cloning characterization of iaaM, a virulence determinant of Pseudomonas savastanoi. J Bacteriol. 1982 Jan;149(1):40–46. doi: 10.1128/jb.149.1.40-46.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Daniels D. L., de Wet J. R., Blattner F. R. New map of bacteriophage lambda DNA. J Virol. 1980 Jan;33(1):390–400. doi: 10.1128/jvi.33.1.390-400.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ely B., Croft R. H. Transposon mutagenesis in Caulobacter crescentus. J Bacteriol. 1982 Feb;149(2):620–625. doi: 10.1128/jb.149.2.620-625.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garfinkel D. J., Nester E. W. Agrobacterium tumefaciens mutants affected in crown gall tumorigenesis and octopine catabolism. J Bacteriol. 1980 Nov;144(2):732–743. doi: 10.1128/jb.144.2.732-743.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hernalsteens J. P., De Greve H., Van Montagu M., Schell J. Mutagenesis by insertion of the drug resistance transposon Tn7 applied to the Ti plasmid of Agrobacterium tumefaciens. Plasmid. 1978 Feb;1(2):218–225. doi: 10.1016/0147-619x(78)90040-9. [DOI] [PubMed] [Google Scholar]
- Howe M. M., Schumm J. W., Taylor A. L. The S and U genes of bacteriophage mu are located in the invertible G segment of mu DNA. Virology. 1979 Jan 15;92(1):108–124. doi: 10.1016/0042-6822(79)90218-6. [DOI] [PubMed] [Google Scholar]
- Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
- Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
- Klapwijk P. M., van Breukelen J., Korevaar K., Ooms G., Schilperoort R. A. Transposition of Tn904 encoding streptomycin resistance into the octopine Ti plasmid of Agrobacterium tumefaciens. J Bacteriol. 1980 Jan;141(1):129–136. doi: 10.1128/jb.141.1.129-136.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee J. H., Heffernan L., Wilcox G. Isolation of ara-lac gene fusions in Salmonella typhimurium LT2 by using transducing bacteriophage Mu d (Apr lac). J Bacteriol. 1980 Sep;143(3):1325–1331. doi: 10.1128/jb.143.3.1325-1331.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meade H. M., Long S. R., Ruvkun G. B., Brown S. E., Ausubel F. M. Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis. J Bacteriol. 1982 Jan;149(1):114–122. doi: 10.1128/jb.149.1.114-122.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murooka Y., Takizawa N., Harada T. Introduction of bacteriophage Mu into bacteria of various genera and intergeneric gene transfer by RP4::Mu. J Bacteriol. 1981 Jan;145(1):358–368. doi: 10.1128/jb.145.1.358-368.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ooms G., Klapwijk P. M., Poulis J. A., Schilperoort R. A. Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens. J Bacteriol. 1980 Oct;144(1):82–91. doi: 10.1128/jb.144.1.82-91.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pischl D. L., Farrand S. K. Transposon-facilitated chromosome mobilization in Agrobacterium tumefaciens. J Bacteriol. 1983 Mar;153(3):1451–1460. doi: 10.1128/jb.153.3.1451-1460.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sato M., Staskawicz B. J., Panopoulos N. J., Peters S., Honma M. A host-dependent hybrid plasmid suitable as a suicidal carrier for transposable elements. Plasmid. 1981 Nov;6(3):325–331. doi: 10.1016/0147-619x(81)90040-8. [DOI] [PubMed] [Google Scholar]
- Shepard H. M., Polisky B. Measurement of Plasmid copy number. Methods Enzymol. 1979;68:503–513. doi: 10.1016/0076-6879(79)68039-4. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- White F. F., Nester E. W. Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes. J Bacteriol. 1980 Mar;141(3):1134–1141. doi: 10.1128/jb.141.3.1134-1141.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]