Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Apr;158(1):49–54. doi: 10.1128/jb.158.1.49-54.1984

Streptococcus faecalis proton gradients and tetracycline transport.

G R Munske, E V Lindley, J A Magnuson
PMCID: PMC215377  PMID: 6325398

Abstract

The transport of chlortetracycline by Streptococcus faecalis is energy dependent. Addition of glucose to energy-depleted cells enhances both the transport rates and accumulation levels. Transport rates can be altered independently of glucose by treating cells with ionophores that increase or decrease the proton gradient. The transport of the antibiotic is linked only to the transmembrane pH difference, delta pH, and not the transmembrane electrical potential, delta psi. This conclusion was verified by quantitative measurements of delta pH, delta psi, and tetracycline accumulation levels. A linear correlation between delta pH and the tetracycline electrochemical potential was observed. Tetracycline most likely accumulates by the symport of protons in which the protons are bound to an anionic form of the antibiotic to form an uncharged molecule.

Full text

PDF
49

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asleson G. L., Frank C. W. pH dependence of carbon-13 nuclear magnetic resonance shifts of tetracycline. Microscopic dissociation constants. J Am Chem Soc. 1976 Aug 4;98(16):4745–4749. doi: 10.1021/ja00432a009. [DOI] [PubMed] [Google Scholar]
  2. Bakker E. P., Mangerich W. E. Interconversion of components of the bacterial proton motive force by electrogenic potassium transport. J Bacteriol. 1981 Sep;147(3):820–826. doi: 10.1128/jb.147.3.820-826.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dockter M. E., Magnuson J. A. Characterization of the active transport of chlorotetracycline in staphylococcus aureus by a fluorescence technique. J Supramol Struct. 1974;2(1):32–44. doi: 10.1002/jss.400020105. [DOI] [PubMed] [Google Scholar]
  4. Harold F. M., Papineau D. Cation transport and electrogenesis by Streptococcus faecalis. I. The membrane potential. J Membr Biol. 1972;8(1):27–44. doi: 10.1007/BF01868093. [DOI] [PubMed] [Google Scholar]
  5. Harold F. M., Papineau D. Cation transport and electrogenesis by Streptococcus faecalis. II. Proton and sodium extrusion. J Membr Biol. 1972;8(1):45–62. doi: 10.1007/BF01868094. [DOI] [PubMed] [Google Scholar]
  6. Harold F. M., Pavlasová E., Baarda J. R. A transmembrane pH gradient in Streptococcus faecalis: origin, and dissipation by proton conductors and N,N'-dicyclohexylcarbodimide. Biochim Biophys Acta. 1970;196(2):235–244. doi: 10.1016/0005-2736(70)90011-8. [DOI] [PubMed] [Google Scholar]
  7. Kashket E. R., Blanchard A. G., Metzger W. C. Proton motive force during growth of Streptococcus lactis cells. J Bacteriol. 1980 Jul;143(1):128–134. doi: 10.1128/jb.143.1.128-134.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kashket E. R., Wilson T. H. Proton-coupled accumulation of galactoside in Streptococcus lactis 7962. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2866–2869. doi: 10.1073/pnas.70.10.2866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mates S. M., Eisenberg E. S., Mandel L. J., Patel L., Kaback H. R., Miller M. H. Membrane potential and gentamicin uptake in Staphylococcus aureus. Proc Natl Acad Sci U S A. 1982 Nov;79(21):6693–6697. doi: 10.1073/pnas.79.21.6693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McMurry L. M., Cullinane J. C., Petrucci R. E., Jr, Levy S. B. Active uptake of tetracycline by membrane vesicles from susceptible Escherichia coli. Antimicrob Agents Chemother. 1981 Sep;20(3):307–313. doi: 10.1128/aac.20.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McMurry L., Petrucci R. E., Jr, Levy S. B. Active efflux of tetracycline encoded by four genetically different tetracycline resistance determinants in Escherichia coli. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3974–3977. doi: 10.1073/pnas.77.7.3974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Niven D. F., Hamilton W. A. Mechanisms of energy coupling to the transport of amino acids by Staphylococcus aureus. Eur J Biochem. 1974 May 15;44(2):517–522. doi: 10.1111/j.1432-1033.1974.tb03510.x. [DOI] [PubMed] [Google Scholar]
  13. Ramos S., Kaback H. R. pH-dependent changes in proton:substrate stoichiometries during active transport in Escherichia coli membrane vesicles. Biochemistry. 1977 Sep 20;16(19):4270–4275. doi: 10.1021/bi00638a022. [DOI] [PubMed] [Google Scholar]
  14. Rottenberg H. The driving force for proton(s) metabolites cotransport in bacterial cells. FEBS Lett. 1976 Jul 15;66(2):159–163. doi: 10.1016/0014-5793(76)80493-0. [DOI] [PubMed] [Google Scholar]
  15. Rottenberg H. The measurement of transmembrane electrochemical proton gradients. J Bioenerg. 1975 May;7(2):61–74. doi: 10.1007/BF01558427. [DOI] [PubMed] [Google Scholar]
  16. Weckesser J., Magnuson J. A. Light-induced, carrier-mediated transport of tetracycline by Rhodopseudomonas sphaeroides. J Bacteriol. 1979 Jun;138(3):678–683. doi: 10.1128/jb.138.3.678-683.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES