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. 1984 Apr;158(1):141–147. doi: 10.1128/jb.158.1.141-147.1984

Functional limits of the araIc promoter suggest an additional regulatory site for araBAD expression.

A H Horwitz, C G Miyada, G Wilcox
PMCID: PMC215391  PMID: 6370954

Abstract

The araBAD promoter is defined, in part, by two types of cis-acting constitutive mutations, araIc at position -35 and araXc at position -10. Subcloning experiments demonstrated that the araIc and araIcXc promoters require DNA sequence information out to position -53 to -56 for maximum constitutive expression. This is 8 to 10 base pairs more DNA than is generally thought to be necessary for RNA polymerase interaction. The -53 to -56 region is required for glucose repression, suggesting that an additional factor interacts in this region and is necessary for maximum expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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