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. 1984 May;158(2):425–429. doi: 10.1128/jb.158.2.425-429.1984

Intracellular localization of photosynthetic membrane growth initiation sites in Rhodopseudomonas sphaeroides.

G S Inamine, J Van Houten, R A Niederman
PMCID: PMC215445  PMID: 6373719

Abstract

Putative membrane invagination sites at which intracytoplasmic photosynthetic membrane growth is initiated in Rhodopseudomonas sphaeroides can be isolated in an upper pigmented fraction by rate-zone sedimentation. The intracellular localization of membranes present in the isolated fraction was investigated with the impermeant surface-labeling reagent pyridoxal 5'-phosphate, which has been shown to diffuse into the periplasmic space and to label proteins of both the peripheral cytoplasmic membrane and the mature intracytoplasmic membrane. A comparison of the extent of labeling at 25 and 0 degrees C was consistent with the possibility that membranes present in the upper pigmented fraction arise from sites near the cell periphery. Pronase digestion of the surface-labeled membranes suggested further that the purified upper fraction consisted largely of open membrane fragments and that the majority of the intracytoplasmic membrane is labeled by this procedure. The pigmented membrane growth initiation sites were separated partially from undifferentiated respiratory cytoplasmic membrane also present in the upper fraction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Broglie R. M., Hunter C. N., Delepelaire P., Niederman R. A., Chua N. H., Clayton R. K. Isolation and characterization of the pigment-protein complexes of Rhodopseudomonas sphaeroides by lithium dodecyl sulfate/polyacrylamide gel electrophoresis. Proc Natl Acad Sci U S A. 1980 Jan;77(1):87–91. doi: 10.1073/pnas.77.1.87. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clayton R. K., Clayton B. J. B850 pigment-protein complex of Rhodopseudomonas sphaeroides: Extinction coefficients, circular dichroism, and the reversible binding of bacteriochlorophyll. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5583–5587. doi: 10.1073/pnas.78.9.5583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Collins M. L., Mallon D. E., Niederman R. A. Assessment of Rhodopseudomonas sphaeroides chromatophore membrane asymmetry through bilateral antiserum adsorption studies. J Bacteriol. 1980 Jul;143(1):221–230. doi: 10.1128/jb.143.1.221-230.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dierstein R., Drews G. Membrane differentiation and assembly of the pigment-protein complexes of the photosynthetic bacterium Rhodopseudomonas capsulata. Prog Clin Biol Res. 1982;102(Pt B):247–256. [PubMed] [Google Scholar]
  5. Francis G. A., Richards W. R. Localization of photosynthetic membrane components in Rhodopseudomonas sphaeroides by a radioactive labeling procedure. Biochemistry. 1980 Oct 28;19(22):5104–5111. doi: 10.1021/bi00563a026. [DOI] [PubMed] [Google Scholar]
  6. Hunter C. N., Holmes N. G., Jones O. T., Niederman R. A. Membranes of Rhodopseudomonas sphaeroides. VII. Photochemical properties of a fraction enriched in newly synthesized bacteriochlorophyll a-protein complexes. Biochim Biophys Acta. 1979 Nov 8;548(2):253–266. doi: 10.1016/0005-2728(79)90133-6. [DOI] [PubMed] [Google Scholar]
  7. Hunter C. N., Pennoyer J. D., Niederman R. A. Assembly and structural organization of pigment-protein complexes in membranes of Rhodopseudomonas sphaeroides. Prog Clin Biol Res. 1982;102(Pt B):257–265. [PubMed] [Google Scholar]
  8. Hunter C. N., van Grondelle R., Holmes N. G., Jones O. T., Niederman R. A. Fluorescence yield properties of a fraction enriched in newly synthesized bacteriochlorophyll a-protein complexes from rhodopseudomonas sphaeroides. Photochem Photobiol. 1979 Aug;30(2):313–316. doi: 10.1111/j.1751-1097.1979.tb07154.x. [DOI] [PubMed] [Google Scholar]
  9. Inamine G. S., Niederman R. A. Development and growth of photosynthetic membranes of Rhodospirillum rubrum. J Bacteriol. 1982 Jun;150(3):1145–1153. doi: 10.1128/jb.150.3.1145-1153.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Inamine G. S., Reilly P. A., Niederman R. A. Differential protein insertion into developing photosynthetic membrane regions of Rhodopseudomonas sphaeroides. J Cell Biochem. 1984;24(1):69–77. doi: 10.1002/jcb.240240106. [DOI] [PubMed] [Google Scholar]
  11. Lommen M. A., Takemoto J. Comparison, by freeze-fracture electron microscopy, of chromatophores, spheroplast-derived membrane vesicles, and whole cells of Rhodopseudomonas sphaeroides. J Bacteriol. 1978 Nov;136(2):730–741. doi: 10.1128/jb.136.2.730-741.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marty-Mazars D., Horiuchi S., Tai P. C., Davis B. D. Proteins of ribosome-bearing and free-membrane domains in Bacillus subtilis. J Bacteriol. 1983 Jun;154(3):1381–1388. doi: 10.1128/jb.154.3.1381-1388.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Niederman R. A., Mallon D. E., Langan J. J. Membranes of Rhodopseudomonas sphaeroides. IV. Assembly of chromatophores in low-aeration cell suspensions. Biochim Biophys Acta. 1976 Aug 13;440(2):429–447. doi: 10.1016/0005-2728(76)90076-1. [DOI] [PubMed] [Google Scholar]
  14. Niederman R. A., Mallon D. E., Parks L. C. Membranes of Rhodopseudomonas sphaeroides. VI. Isolation of a fraction enriched in newly synthesized bacteriochlorophyll alpha-protein complexes. Biochim Biophys Acta. 1979 Aug 7;555(2):210–220. doi: 10.1016/0005-2736(79)90161-5. [DOI] [PubMed] [Google Scholar]
  15. Okamura M. Y., Steiner L. A., Feher G. Characterization of reaction centers from photosynthetic bacteria. I. Subunit structure of the protein mediating the primary photochemistry in Rhodopseudomonas spheroides R-26. Biochemistry. 1974 Mar 26;13(7):1394–1403. doi: 10.1021/bi00704a013. [DOI] [PubMed] [Google Scholar]
  16. Onishi J. C., Niederman R. A. Rhodopseudomonas sphaeroides membranes: alterations in phospholipid composition in aerobically and phototrophically grown cells. J Bacteriol. 1982 Mar;149(3):831–839. doi: 10.1128/jb.149.3.831-839.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Parks L. C., Niederman R. A. Membranes of Rhodopseudomonas sphaeroides. V. Identification of bacteriochlorophyll alpha-depleted cytoplasmic membrane in phototrophically grown cells. Biochim Biophys Acta. 1978 Jul 20;511(1):70–82. doi: 10.1016/0005-2736(78)90065-2. [DOI] [PubMed] [Google Scholar]
  18. Pradel J., Lavergne J., Moya I. Formation and development of photosynthetic units in repigmenting Rhodopseudomonas sphaeroides wild type and "Phofil" mutant strain. Biochim Biophys Acta. 1978 May 10;502(2):169–182. doi: 10.1016/0005-2728(78)90039-7. [DOI] [PubMed] [Google Scholar]
  19. Takemoto J. Kinetics of photosynthetic membrane protein assembly in Rhodopseudomonas spheroides. Arch Biochem Biophys. 1974 Aug;163(2):515–520. doi: 10.1016/0003-9861(74)90509-8. [DOI] [PubMed] [Google Scholar]

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