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. 1984 May;158(2):609–614. doi: 10.1128/jb.158.2.609-614.1984

Oxygen-dependent proton efflux in cyanobacteria (blue-green algae).

S Scherer, E Stürzl, P Böger
PMCID: PMC215472  PMID: 6327614

Abstract

The oxygen-dependent proton efflux (in the dark) of intact cells of Anabaena variabilis and four other cyanobacteria (blue-green algae) was investigated. In contrast to bacteria and isolated mitochondria, an H+/e ratio (= protons translocated per electron transported) of only 0.23 to 0.35 and a P/e ratio of 0.8 to 1.5 were observed, indicative of respiratory electron transport being localized essentially on the thylakoids, not on the cytoplasmic membrane. Oxygen-induced acidification of the medium was sensitive to cyanide and the uncoupler carbonyl cyanide m-chlorophenylhydrazone. Inhibitors such as 2,6-dinitrophenol and vanadate exhibited a significant decrease in the H+/e ratio. After the oxygen pulse, electron transport started immediately, but proton efflux lagged 40 to 60 s behind, a period also needed before maximum ATP pool levels were attained. We suggest that proton efflux in A. variabilis is due to a proton-translocating ATP hydrolase (ATP-consuming ATPase) rather than to respiratory electron transport located on the cytoplasmic membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexandre A., Reynafarje B., Lehninger A. L. Stoichiometry of vectorial H+ movements coupled to electron transport and to ATP synthesis in mitochondria. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5296–5300. doi: 10.1073/pnas.75.11.5296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Binder A. Respiration and photosynthesis in energy-transducing membranes of cyanobacteria. J Bioenerg Biomembr. 1982 Dec;14(5-6):271–286. doi: 10.1007/BF00743057. [DOI] [PubMed] [Google Scholar]
  3. Bisalputra T., Brown D. L., Weier T. E. Possible respiratory sites in a blue-green alga Nostoc sphaericum as demonstrated by potassium tellurite and tetranitro-blue tetrazolium reduction. J Ultrastruct Res. 1969 Apr;27(2):182–197. [PubMed] [Google Scholar]
  4. Fillingame R. H. The proton-translocating pumps of oxidative phosphorylation. Annu Rev Biochem. 1980;49:1079–1113. doi: 10.1146/annurev.bi.49.070180.005243. [DOI] [PubMed] [Google Scholar]
  5. Jones C. W., Brice J. M., Downs A. J., Drozd J. W. Bacterial respiration-linked proton translocation and its relationship to respiratory-chain composition. Eur J Biochem. 1975 Mar 17;52(2):265–271. doi: 10.1111/j.1432-1033.1975.tb03994.x. [DOI] [PubMed] [Google Scholar]
  6. Lee-Kaden J., Simonis W. Amino acid uptake and energy coupling dependent on photosynthesis in Anacystis nidulans. J Bacteriol. 1982 Jul;151(1):229–236. doi: 10.1128/jb.151.1.229-236.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Meyer D. J., Jones C. W. Oxidative phosphorylation in bacteria which contain different cytochrome oxidases. Eur J Biochem. 1973 Jul 2;36(1):144–151. doi: 10.1111/j.1432-1033.1973.tb02894.x. [DOI] [PubMed] [Google Scholar]
  8. Mitchell P., Moyle J. Acid-base titration across the membrane system of rat-liver mitochondria. Catalysis by uncouplers. Biochem J. 1967 Aug;104(2):588–600. doi: 10.1042/bj1040588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Paschinger H. DCCD induced sodium uptake by Anacystis nidulans. Arch Microbiol. 1977 Jun 20;113(3):285–291. doi: 10.1007/BF00492037. [DOI] [PubMed] [Google Scholar]
  10. Peschek G. A. Proton pump coupled to cytochrome c oxidase in the cyanobacterium Anacystis nidulans. J Bacteriol. 1983 Jan;153(1):539–542. doi: 10.1128/jb.153.1.539-542.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Peschek G. A., Schmetterer G. Evidence for plastoquinol-cytochrome f/b-563 reductase as a common electron donor to P700 and cytochrome oxidase in cyanobacteria. Biochem Biophys Res Commun. 1982 Oct 15;108(3):1188–1195. doi: 10.1016/0006-291x(82)92126-x. [DOI] [PubMed] [Google Scholar]
  12. Prochaska L. J., Bisson R., Capaldi R. A., Steffens G. C., Buse G. Inhibition of cytochrome c oxidase function by dicyclohexylcarbodiimide. Biochim Biophys Acta. 1981 Sep 14;637(2):360–373. doi: 10.1016/0005-2728(81)90175-4. [DOI] [PubMed] [Google Scholar]
  13. Raboy B., Padan E. Active transport of glucose and alpha-methylglucoside in the cyanobacterium Plectonema boryanum. J Biol Chem. 1978 May 10;253(9):3287–3291. [PubMed] [Google Scholar]
  14. Reed R. H., Rowell P., Stewart W. D. Characterization of the transport of potassium ions in the cyanobacterium Anabaena variabilis Kütz. Eur J Biochem. 1981 May 15;116(2):323–330. doi: 10.1111/j.1432-1033.1981.tb05337.x. [DOI] [PubMed] [Google Scholar]
  15. Scholes P., Mitchell P., Moyle J. The polarity of proton translocation in some photosynthetic microorganisms. Eur J Biochem. 1969 Apr;8(3):450–454. doi: 10.1111/j.1432-1033.1969.tb00548.x. [DOI] [PubMed] [Google Scholar]

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