Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 May;158(2):665–673. doi: 10.1128/jb.158.2.665-673.1984

D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.

J E Lopilato, J L Garwin, S D Emr, T J Silhavy, J R Beckwith
PMCID: PMC215481  PMID: 6327616

Abstract

We have isolated mutants defective in high-affinity D-ribose transport. The mutations map in rbsT or rbsB , the structural gene for ribose binding protein. rbsT consists of at least one gene coding for a protein required for high-affinity transport. The high-affinity transport-defective mutants were able to utilize D-ribose, indicating that at least a second, low-affinity transport system for D-ribose is present in Escherichia coli K-12. rbsT and rbsB are located at min 84 on the E. coli genetic map and, together with rbsK , the gene coding for ribokinase , constitute an rbs operon. The order of genes is rbsP /O rbsT rbsB rbsK . The rbs operon is subject to negative control by the product of the rbsR gene. rbsR is located distal to the rbs operon and appears to form a separate transcriptional unit.

Full text

PDF
665

Images in this article

667Image
on p.667
668Image
on p.668
671Image
on p.671
671Image
on p.671

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson A., Cooper R. A. Biochemical and genetical studies on ribose catabolism in Escherichia coli K12. J Gen Microbiol. 1970 Aug;62(3):335–339. doi: 10.1099/00221287-62-3-335. [DOI] [PubMed] [Google Scholar]
  2. Bochner B. R., Huang H. C., Schieven G. L., Ames B. N. Positive selection for loss of tetracycline resistance. J Bacteriol. 1980 Aug;143(2):926–933. doi: 10.1128/jb.143.2.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
  6. Chamberlain J. P. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979 Sep 15;98(1):132–135. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
  7. Chumley F. G., Menzel R., Roth J. R. Hfr formation directed by tn10. Genetics. 1979 Apr;91(4):639–655. doi: 10.1093/genetics/91.4.639. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Curtis S. J. Mechanism of energy coupling for transport of D-ribose in Escherichia coli. J Bacteriol. 1974 Oct;120(1):295–303. doi: 10.1128/jb.120.1.295-303.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dills S. S., Apperson A., Schmidt M. R., Saier M. H., Jr Carbohydrate transport in bacteria. Microbiol Rev. 1980 Sep;44(3):385–418. doi: 10.1128/mr.44.3.385-418.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Emr S. D., Hanley-Way S., Silhavy T. J. Suppressor mutations that restore export of a protein with a defective signal sequence. Cell. 1981 Jan;23(1):79–88. doi: 10.1016/0092-8674(81)90272-5. [DOI] [PubMed] [Google Scholar]
  11. Galloway D. R., Furlong C. E. The role of ribose-binding protein in transport and chemotaxis in Escherichia coli K12. Arch Biochem Biophys. 1977 Dec;184(2):496–504. doi: 10.1016/0003-9861(77)90459-3. [DOI] [PubMed] [Google Scholar]
  12. Garwin J. L., Beckwith J. Secretion and processing of ribose-binding protein in Escherichia coli. J Bacteriol. 1982 Feb;149(2):789–792. doi: 10.1128/jb.149.2.789-792.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Groarke J. M., Mahoney W. C., Hope J. N., Furlong C. E., Robb F. T., Zalkin H., Hermodson M. A. The amino acid sequence of D-ribose-binding protein from Escherichia coli K12. J Biol Chem. 1983 Nov 10;258(21):12952–12956. [PubMed] [Google Scholar]
  14. Harayama S., Bollinger J., Iino T., Hazelbauer G. L. Characterization of the mgl operon of Escherichia coli by transposon mutagenesis and molecular cloning. J Bacteriol. 1983 Jan;153(1):408–415. doi: 10.1128/jb.153.1.408-415.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Heyneker H. L., Shine J., Goodman H. M., Boyer H. W., Rosenberg J., Dickerson R. E., Narang S. A., Itakura K., Lin S., Riggs A. D. Synthetic lac operator DNA is functional in vivo. Nature. 1976 Oct 28;263(5580):748–752. doi: 10.1038/263748a0. [DOI] [PubMed] [Google Scholar]
  16. Higgins C. F., Haag P. D., Nikaido K., Ardeshir F., Garcia G., Ames G. F. Complete nucleotide sequence and identification of membrane components of the histidine transport operon of S. typhimurium. Nature. 1982 Aug 19;298(5876):723–727. doi: 10.1038/298723a0. [DOI] [PubMed] [Google Scholar]
  17. Hofnung M., Hatfield D., Schwartz M. malB region in Escherichia coli K-12: characterization of new mutations. J Bacteriol. 1974 Jan;117(1):40–47. doi: 10.1128/jb.117.1.40-47.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Iida A., Harayama S., Iino T., Hazelbauer G. L. Molecular cloning and characterization of genes required for ribose transport and utilization in Escherichia coli K-12. J Bacteriol. 1984 May;158(2):674–682. doi: 10.1128/jb.158.2.674-682.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ito K., Bassford P. J., Jr, Beckwith J. Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins? Cell. 1981 Jun;24(3):707–717. doi: 10.1016/0092-8674(81)90097-0. [DOI] [PubMed] [Google Scholar]
  20. Ito K., Sato T., Yura T. Synthesis and assembly of the membrane proteins in E. coli. Cell. 1977 Jul;11(3):551–559. doi: 10.1016/0092-8674(77)90073-3. [DOI] [PubMed] [Google Scholar]
  21. Kleckner N., Reichardt K., Botstein D. Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10. J Mol Biol. 1979 Jan 5;127(1):89–115. doi: 10.1016/0022-2836(79)90461-3. [DOI] [PubMed] [Google Scholar]
  22. Kleckner N., Roth J., Botstein D. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J Mol Biol. 1977 Oct 15;116(1):125–159. doi: 10.1016/0022-2836(77)90123-1. [DOI] [PubMed] [Google Scholar]
  23. Komeda Y., Iino T. Regulation of expression of the flagellin gene (hag) in Escherichia coli K-12: analysis of hag-lac gene fusions. J Bacteriol. 1979 Sep;139(3):721–729. doi: 10.1128/jb.139.3.721-729.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  25. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Marsh R. C. Map location of the Escherichia coli origin of replication. Mol Gen Genet. 1978 Nov 9;166(3):299–304. doi: 10.1007/BF00267622. [DOI] [PubMed] [Google Scholar]
  27. Murray N. E., Brammar W. J., Murray K. Lambdoid phages that simplify the recovery of in vitro recombinants. Mol Gen Genet. 1977 Jan 7;150(1):53–61. doi: 10.1007/BF02425325. [DOI] [PubMed] [Google Scholar]
  28. Neu H. C., Heppel L. A. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J Biol Chem. 1965 Sep;240(9):3685–3692. [PubMed] [Google Scholar]
  29. Oliver D. B., Beckwith J. E. coli mutant pleiotropically defective in the export of secreted proteins. Cell. 1981 Sep;25(3):765–772. doi: 10.1016/0092-8674(81)90184-7. [DOI] [PubMed] [Google Scholar]
  30. Panyim S., Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969 Mar;130(1):337–346. doi: 10.1016/0003-9861(69)90042-3. [DOI] [PubMed] [Google Scholar]
  31. Raibaud O., Roa M., Braun-Breton C., Schwartz M. Structure of the malB region in Escherichia coli K12. I. Genetic map of the malK-lamB operon. Mol Gen Genet. 1979 Jul 24;174(3):241–248. doi: 10.1007/BF00267796. [DOI] [PubMed] [Google Scholar]
  32. Sarthy A., Michaelis S., Beckwith J. Use of gene fusions to determine the orientation of gene phoA on the Escherichia coli chromosome. J Bacteriol. 1981 Jan;145(1):293–298. doi: 10.1128/jb.145.1.293-298.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Silhavy T. J., Brickman E., Bassford P. J., Jr, Casadaban M. J., Shuman H. A., Schwartz V., Guarente L., Schwartz M., Beckwith J. R. Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon. Mol Gen Genet. 1979 Jul 24;174(3):249–259. doi: 10.1007/BF00267797. [DOI] [PubMed] [Google Scholar]
  34. Southern E. Gel electrophoresis of restriction fragments. Methods Enzymol. 1979;68:152–176. doi: 10.1016/0076-6879(79)68011-4. [DOI] [PubMed] [Google Scholar]
  35. Sternberg N., Tiemeier D., Enquist L. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1. Gene. 1977 May;1(3-4):255–280. doi: 10.1016/0378-1119(77)90049-x. [DOI] [PubMed] [Google Scholar]
  36. Taylor A. L., Trotter C. D. Revised linkage map of Escherichia coli. Bacteriol Rev. 1967 Dec;31(4):332–353. doi: 10.1128/br.31.4.332-353.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Wanner B. L., Bernstein J. Determining the phoM map location in Escherichia coli K-12 by using a nearby transposon Tn10 insertion. J Bacteriol. 1982 Apr;150(1):429–432. doi: 10.1128/jb.150.1.429-432.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Willis R. C., Furlong C. E. Purification and properties of a ribose-binding protein from Escherichia coli. J Biol Chem. 1974 Nov 10;249(21):6926–6929. [PubMed] [Google Scholar]
  39. von Meyenburg K., Hansen F. G., Nielsin L. D., Riise E. Origin of replication, oriC, or the Escherichia coli chromosome on specialized transducing phages lambda asn. Mol Gen Genet. 1978 Apr 17;160(3):287–295. doi: 10.1007/BF00332972. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES