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. 1984 Jun;158(3):860–865. doi: 10.1128/jb.158.3.860-865.1984

Cystathionine accumulation in Saccharomyces cerevisiae.

B Ono, T Suruga, M Yamamoto, S Yamamoto, K Murata, A Kimura, S Shinoda, S Ohmori
PMCID: PMC215520  PMID: 6373742

Abstract

A cysteine-dependent strain of Saccharomyces cerevisiae and its prototrophic revertants accumulated cystathionine in cells. The cystathionine accumulation was caused by a single mutation having a high incidence of gene conversion. The mutation was designated cys3 and was shown to cause loss of gamma-cystathionase activity. Cysteine dependence of the initial strain was determined by two linked and interacting mutations, cys3 and cys1 . Since cys1 mutations cause a loss of serine acetyltransferase activity, our observation led to the conclusion that S. cerevisiae synthesizes cysteine by sulfhydrylation of serine with hydrogen sulfide and by cleavage of cystathionine which is synthesized from serine and homocysteine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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