Abstract
Mycoplasma mycoides subsp. mycoides has been shown to possess an unusual capacity for the uptake and utilization of exogenous deoxyribonucleoside 5'-monophosphates intact without prior dephosphorylation. In this study, it was found that once inside the cell, deoxyribonucleoside 5'-monophosphates were rapidly phosphorylated to the triphosphate level and incorporated into DNA. Catabolism of deoxyribonucleoside 5'-monophosphates was also observed. Competition studies indicated that a single uptake system with a higher affinity for deoxyribonucleotides mediates the uptake of nucleoside 5'-monophosphates.
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- Bagnara A. S., Finch L. R. Relationships between intracellular contents of nucleotides and 5-phosphoribosyl 1-pyrophosphate in Escherichia coli. Eur J Biochem. 1973 Jul 16;36(2):422–427. doi: 10.1111/j.1432-1033.1973.tb02927.x. [DOI] [PubMed] [Google Scholar]
- FINCH L. R., HIRD F. J. The uptake of amino acids by isolated segments of rat intestine. II. A survey of affinity for uptake from rates of uptake and competition for uptake. Biochim Biophys Acta. 1960 Sep 23;43:278–287. doi: 10.1016/0006-3002(60)90438-8. [DOI] [PubMed] [Google Scholar]
- Mitchell A., Finch L. R. Pathways of nucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides. J Bacteriol. 1977 Jun;130(3):1047–1054. doi: 10.1128/jb.130.3.1047-1054.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitchell A., Sin I. L., Finch L. R. Enzymes of purine metabolism in Mycoplasma mycoides subsp. mycoides. J Bacteriol. 1978 Jun;134(3):706–712. doi: 10.1128/jb.134.3.706-712.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neale G. A., Mitchell A., Finch L. R. Enzymes of pyrimidine deoxyribonucleotide metabolism in Mycoplasma mycoides subsp. mycoides. J Bacteriol. 1983 Dec;156(3):1001–1005. doi: 10.1128/jb.156.3.1001-1005.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neale G. A., Mitchell A., Finch L. R. Pathways of pyrimidine deoxyribonucleotide biosynthesis in Mycoplasma mycoides subsp. mycoides. J Bacteriol. 1983 Apr;154(1):17–22. doi: 10.1128/jb.154.1.17-22.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Razin S. Physiology of mycoplasmas. Adv Microb Physiol. 1973;10:1–80. doi: 10.1016/s0065-2911(08)60086-7. [DOI] [PubMed] [Google Scholar]
- Reynolds E. C., Finch L. R. Estimation of [32P]deoxyribonucleoside triphosphates in cell extracts using periodate treatment. Anal Biochem. 1977 Oct;82(2):591–595. doi: 10.1016/0003-2697(77)90200-7. [DOI] [PubMed] [Google Scholar]
- Rittenberg S. C., Langley D. Utilization of nucleoside monophosphates per Se for intraperiplasmic growth of Bdellovibrio bacteriovorus. J Bacteriol. 1975 Mar;121(3):1137–1144. doi: 10.1128/jb.121.3.1137-1144.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILBRANDT W. The relation between rate and affinity in carrier transports. J Cell Physiol. 1956 Feb;47(1):137–145. doi: 10.1002/jcp.1030470109. [DOI] [PubMed] [Google Scholar]