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. 1984 Jun;158(3):978–982. doi: 10.1128/jb.158.3.978-982.1984

Cloning and characterization of the Bacillus licheniformis gene coding for alkaline phosphatase.

F M Hulett
PMCID: PMC215538  PMID: 6327655

Abstract

The structural gene for alkaline phosphatase (orthophosphoric monoester phosphohydrolase; EC 3.1.3.1) of Bacillus licheniformis MC14 was cloned into the Pst1 site of pMK2004 from chromosomal DNA. The gene was cloned on an 8.5-kilobase DNA fragment. A restriction map was developed, and the gene was subcloned on a 4.2-kilobase DNA fragment. The minimum coding region of the gene was localized to a 1.3-kilobase region. Western blot analysis was used to show that the gene coded for a 60,000-molecular-weight protein which cross-reacts with anti-alkaline phosphatase prepared against the salt-extractable membrane alkaline phosphatase of B. licheniformis MC14 .

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Selected References

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