Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Jun;158(3):997–1004. doi: 10.1128/jb.158.3.997-1004.1984

Physical and genetic analyses of the Inc-I alpha plasmid R64.

T Furuichi, T Komano, T Nisioka
PMCID: PMC215541  PMID: 6327656

Abstract

A 126-kilobase (kb) physical and genetic map of the Inc-I alpha plasmid R64 was constructed by using the restriction enzymes, BamHI, SalI, XhoI, HindIII, and EcoRI. The replication (Rep) and incompatability (Inc) functions of this plasmid were located in a 1.75-kb segment of an EcoRI fragment, E10 (3.3 kb). In addition, the genes determining growth inhibition of phage BF23 (Ibf), suppression of dnaG ( Sog ), resistance to tetracycline (Tetr), and resistance to streptomycin ( Strr ) were located on the 5.5-kb HindIII-XhoI fragment, the 8.1-kb EcoRI fragment (E5), the 4.6-kb HindIII fragment (H8), and the 4.1-kb HindIII fragment (H10), respectively. The map of R64 was compared with that of ColIb, which belongs to the Inc-I alpha group.

Full text

PDF
997

Images in this article

998Image
on p.998
999Image
on p.999

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Auerswald E. A., Ludwig G., Schaller H. Structural analysis of Tn5. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 1):107–113. doi: 10.1101/sqb.1981.045.01.019. [DOI] [PubMed] [Google Scholar]
  2. Bannister D., Glover S. W. Restriction and modification of bacteriophages by R+ strains of Escherichia coli K12. Biochem Biophys Res Commun. 1968 Mar 27;30(6):735–738. doi: 10.1016/0006-291x(68)90575-5. [DOI] [PubMed] [Google Scholar]
  3. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  5. Boulnois G. J., Wilkins B. M. A novel priming system for conjugal synthesis of an IncI alpha plasmid in recipients. Mol Gen Genet. 1979 Oct 1;175(3):275–279. doi: 10.1007/BF00397227. [DOI] [PubMed] [Google Scholar]
  6. Boyer H. W., Roulland-Dussoix D. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969 May 14;41(3):459–472. doi: 10.1016/0022-2836(69)90288-5. [DOI] [PubMed] [Google Scholar]
  7. Chang A. C., Cohen S. N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978 Jun;134(3):1141–1156. doi: 10.1128/jb.134.3.1141-1156.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chatfield L. K., Orr E., Boulnois G. J., Wilkins B. M. DNA primase of plasmid ColIb is involved in conjugal DnA synthesis in donor and recipient bacteria. J Bacteriol. 1982 Dec;152(3):1188–1195. doi: 10.1128/jb.152.3.1188-1195.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Clarke L., Carbon J. Functional expression of cloned yeast DNA in Escherichia coli: specific complementation of argininosuccinate lyase (argH) mutations. J Mol Biol. 1978 Apr 25;120(4):517–532. doi: 10.1016/0022-2836(78)90351-0. [DOI] [PubMed] [Google Scholar]
  10. Collins J., Brüning H. J. Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage lambda: "cosmids". Gene. 1978 Oct;4(2):85–107. doi: 10.1016/0378-1119(78)90023-9. [DOI] [PubMed] [Google Scholar]
  11. Dalrymple B. P., Boulnois G. J., Wilkins B. M., Orr E., Williams P. H. Evidence for two genetically distinct DNA primase activities specified by plasmids of the B and I incompatibility groups. J Bacteriol. 1982 Jul;151(1):1–7. doi: 10.1128/jb.151.1.1-7.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. De Lucia P., Cairns J. Isolation of an E. coli strain with a mutation affecting DNA polymerase. Nature. 1969 Dec 20;224(5225):1164–1166. doi: 10.1038/2241164a0. [DOI] [PubMed] [Google Scholar]
  13. Falkow S., Guerry P., Hedges R. W., Datta N. Polynucleotide sequence relationships among plasmids of the I compatibility complex. J Gen Microbiol. 1974 Nov;85(1):65–76. doi: 10.1099/00221287-85-1-65. [DOI] [PubMed] [Google Scholar]
  14. Hedges R. W., Datta N. Plasmids determining I pili constitute a compatibility complex. J Gen Microbiol. 1973 Jul;77(1):19–25. doi: 10.1099/00221287-77-1-19. [DOI] [PubMed] [Google Scholar]
  15. Hohn B., Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene. 1980 Nov;11(3-4):291–298. doi: 10.1016/0378-1119(80)90069-4. [DOI] [PubMed] [Google Scholar]
  16. Hohn B. In vitro packaging of lambda and cosmid DNA. Methods Enzymol. 1979;68:299–309. doi: 10.1016/0076-6879(79)68021-7. [DOI] [PubMed] [Google Scholar]
  17. Hughes V., Meynell G. G. The contribution of plasmid and host genes to plasmid-mediated interference with phage growth. Genet Res. 1977 Oct;30(2):179–185. doi: 10.1017/s0016672300017572. [DOI] [PubMed] [Google Scholar]
  18. Jorgensen R. A., Berg D. E., Allet B., Reznikoff W. S. Restriction enzyme cleavage map of Tn10, a transposon which encodes tetracycline resistance. J Bacteriol. 1979 Jan;137(1):681–685. doi: 10.1128/jb.137.1.681-685.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
  20. Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
  21. Kingsbury D. T., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . J Bacteriol. 1973 Jun;114(3):1116–1124. doi: 10.1128/jb.114.3.1116-1124.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Lanka E., Scherzinger E., Günther E., Schuster H. A DNA primase specified by I-like plasmids. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3632–3636. doi: 10.1073/pnas.76.8.3632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mendez B., Tachibana C., Levy S. B. Heterogeneity of tetracycline resistance determinants. Plasmid. 1980 Mar;3(2):99–108. doi: 10.1016/0147-619x(80)90101-8. [DOI] [PubMed] [Google Scholar]
  24. Nisioka T., Ozeki H. Early abortive lysis by phage BF23 in Escherichia coli K-12 carrying the colicin Ib factor. J Virol. 1968 Nov;2(11):1249–1254. doi: 10.1128/jvi.2.11.1249-1254.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Oka A., Takanami M. Cleavage map of colicin E1 plasmid. Nature. 1976 Nov 11;264(5582):193–196. doi: 10.1038/264193a0. [DOI] [PubMed] [Google Scholar]
  26. Ramsden M., Strike P. A restriction map of the IncI 1 plasmid TP110. Plasmid. 1982 Jul;8(1):83–85. doi: 10.1016/0147-619x(82)90043-9. [DOI] [PubMed] [Google Scholar]
  27. Sasakawa C., Uno Y., Yoshikawa M. The requirement for both DNA polymerase and 5' to 3' exonuclease activities of DNA polymerase I during Tn5 transposition. Mol Gen Genet. 1981;182(1):19–24. doi: 10.1007/BF00422761. [DOI] [PubMed] [Google Scholar]
  28. Sasakawa C., Yoshikawa M. Requirements for suppression of a dnaG mutation by an I-type plasmid. J Bacteriol. 1978 Feb;133(2):485–491. doi: 10.1128/jb.133.2.485-491.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Scheer-Abramowitz J., Gryczan T. J., Dubnau D. Origin and mode of replication of plasmids pE194 and pUB110. Plasmid. 1981 Jul;6(1):67–77. doi: 10.1016/0147-619x(81)90054-8. [DOI] [PubMed] [Google Scholar]
  30. Sharp P. A., Cohen S. N., Davidson N. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. II. Structure of drug resistance (R) factors and F factors. J Mol Biol. 1973 Apr 5;75(2):235–255. doi: 10.1016/0022-2836(73)90018-1. [DOI] [PubMed] [Google Scholar]
  31. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  32. Timmis K. N., Danbara H., Brady G., Lurz R. Inheritance functions of group IncFII transmissible antibiotic resistance plasmids. Plasmid. 1981 Jan;5(1):53–75. doi: 10.1016/0147-619x(81)90077-9. [DOI] [PubMed] [Google Scholar]
  33. Timmis K. N., Danbara H., Brady G., Lurz R. Inheritance functions of group IncFII transmissible antibiotic resistance plasmids. Plasmid. 1981 Jan;5(1):53–75. doi: 10.1016/0147-619x(81)90077-9. [DOI] [PubMed] [Google Scholar]
  34. Timmis K., Cabello F., Cohen S. N. Cloning, isolation, and characterization of replication regions of complex plasmid genomes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2242–2246. doi: 10.1073/pnas.72.6.2242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Vapnek D., Lipman M. B., Rupp W. D. Physical properties and mechanism of transfer of R factors in Escherichia coli. J Bacteriol. 1971 Oct;108(1):508–514. doi: 10.1128/jb.108.1.508-514.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Yee T., Inouye M. Two-dimensional DNA electrophoresis applied to the study of DNA methylation and the analysis of genome size in Myxococcus xanthus. J Mol Biol. 1982 Jan 15;154(2):181–196. doi: 10.1016/0022-2836(82)90059-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES