Abstract
Strains of Caulobacter crescentus, Pseudomonas putida, Acinetobacter calcoaceticus, Rhizobium meliloti, and Rhodopseudomonas sphaeroides carrying the kanamycin resistance-encoding transposon Tn5 were 15 to 500 times more resistant to streptomycin than transposon-free strains. The streptomycin resistance determinant, which is separable from the kanamycin resistance determinant of Tn5, was not expressed in Escherichia coli or Klebsiella aerogenes.
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Selected References
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- Adams J., Kinney T., Thompson S., Rubin L., Helling R. B. Frequency-Dependent Selection for Plasmid-Containing Cells of ESCHERICHIA COLI. Genetics. 1979 Apr;91(4):627–637. doi: 10.1093/genetics/91.4.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bagdasarian M., Lurz R., Rückert B., Franklin F. C., Bagdasarian M. M., Frey J., Timmis K. N. Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene. 1981 Dec;16(1-3):237–247. doi: 10.1016/0378-1119(81)90080-9. [DOI] [PubMed] [Google Scholar]
- Barrett J. T., Croft R. H., Ferber D. M., Gerardot C. J., Schoenlein P. V., Ely B. Genetic mapping with Tn5-derived auxotrophs of Caulobacter crescentus. J Bacteriol. 1982 Aug;151(2):888–898. doi: 10.1128/jb.151.2.888-898.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
- Juni E. Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus. J Bacteriol. 1972 Nov;112(2):917–931. doi: 10.1128/jb.112.2.917-931.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'neill E. A., Berlinberg C., Bender R. A. Activity of Plasmid Replicons in CAULOBACTER CRESCENTUS : Rp4 and Cole1. Genetics. 1983 Apr;103(4):593–604. doi: 10.1093/genetics/103.4.593. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Putnoky P., Kiss G. B., Ott I., Kondorosi A. Tn5 carries a streptomycin resistance determinant downstream from the kanamycin resistance gene. Mol Gen Genet. 1983;191(2):288–294. doi: 10.1007/BF00334828. [DOI] [PubMed] [Google Scholar]
- Streicher S. L., Bender R. A., Magasanik B. Genetic control of glutamine synthetase in Klebiella aerogenes. J Bacteriol. 1975 Jan;121(1):320–331. doi: 10.1128/jb.121.1.320-331.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wheelis M. L., Stanier R. Y. The genetic control of dissimilatory pathways in Pseudomonas putida. Genetics. 1970 Oct;66(2):245–266. doi: 10.1093/genetics/66.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]