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. 1984 Jul;159(1):421–423. doi: 10.1128/jb.159.1.421-423.1984

Kinetics of methylation in Escherichia coli K-12.

S M Lyons, P F Schendel
PMCID: PMC215653  PMID: 6376478

Abstract

Newly synthesized DNA is undermethylated in E. coli K-12. The amount of N6-methyl deoxyadenylic acid in labeled DNA varied from 0.3 mol% of total adenine for a 2-min pulse to 1.7 mol% for DNA that was labeled for more than two generations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson M. L. Number and size distribution of the DNA chains in Escherichia coli. J Mol Biol. 1978 Jan 15;118(2):227–240. doi: 10.1016/0022-2836(78)90414-x. [DOI] [PubMed] [Google Scholar]
  2. Brewin N., Cairns J. State of the DNA replication fork during thymine deprivation of Escherichia coli, as observed by pulse-labelling with [3H]thymidine. J Mol Biol. 1977 Apr 15;111(3):353–363. doi: 10.1016/s0022-2836(77)80057-0. [DOI] [PubMed] [Google Scholar]
  3. Brewin N. Origin and fate of the small DNA chains synthesized in bacteria after thymine deprivation. J Mol Biol. 1977 Apr 15;111(3):343–352. doi: 10.1016/s0022-2836(77)80056-9. [DOI] [PubMed] [Google Scholar]
  4. Eggertsson G., Adelberg E. A. Map positions and specificities of suppressor mutations in Escherichia coli K-12. Genetics. 1965 Aug;52(2):319–340. doi: 10.1093/genetics/52.2.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Geier G. E., Modrich P. Recognition sequence of the dam methylase of Escherichia coli K12 and mode of cleavage of Dpn I endonuclease. J Biol Chem. 1979 Feb 25;254(4):1408–1413. [PubMed] [Google Scholar]
  6. Gelinas R. E., Myers P. A., Roberts R. J. Two sequence-specific endonucleases from Moraxella bovis. J Mol Biol. 1977 Jul;114(1):169–179. doi: 10.1016/0022-2836(77)90290-x. [DOI] [PubMed] [Google Scholar]
  7. Glickman B. W., Radman M. Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063–1067. doi: 10.1073/pnas.77.2.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Glickman B. W. Spontaneous mutagenesis in Escherichia coli strains lacking 6-methyladenine residues in their DNA: an altered mutational spectrum in dam- mutants. Mutat Res. 1979 Jul;61(2):153–162. doi: 10.1016/0027-5107(79)90122-2. [DOI] [PubMed] [Google Scholar]
  9. Glickman B., van den Elsen P., Radman M. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance. Mol Gen Genet. 1978 Jul 25;163(3):307–312. doi: 10.1007/BF00271960. [DOI] [PubMed] [Google Scholar]
  10. Herman G. E., Modrich P. Escherichia coli K-12 clones that overproduce dam methylase are hypermutable. J Bacteriol. 1981 Jan;145(1):644–646. doi: 10.1128/jb.145.1.644-646.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Konrad E. B. Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications. J Bacteriol. 1977 Apr;130(1):167–172. doi: 10.1128/jb.130.1.167-172.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lacks S., Greenberg B. Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol. 1977 Jul;114(1):153–168. doi: 10.1016/0022-2836(77)90289-3. [DOI] [PubMed] [Google Scholar]
  13. Marinus M. G. Adenine methylation of Okazaki fragments in Escherichia coli. J Bacteriol. 1976 Dec;128(3):853–854. doi: 10.1128/jb.128.3.853-854.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Marinus M. G., Morris N. R. Isolation of deoxyribonucleic acid methylase mutants of Escherichia coli K-12. J Bacteriol. 1973 Jun;114(3):1143–1150. doi: 10.1128/jb.114.3.1143-1150.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Neidhardt F. C., Bloch P. L., Smith D. F. Culture medium for enterobacteria. J Bacteriol. 1974 Sep;119(3):736–747. doi: 10.1128/jb.119.3.736-747.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nevers P., Spatz H. C. Escherichia coli mutants uvr D and uvr E deficient in gene conversion of lambda-heteroduplexes. Mol Gen Genet. 1975 Aug 27;139(3):233–243. doi: 10.1007/BF00268974. [DOI] [PubMed] [Google Scholar]
  17. Pukkila P. J., Peterson J., Herman G., Modrich P., Meselson M. Effects of high levels of DNA adenine methylation on methyl-directed mismatch repair in Escherichia coli. Genetics. 1983 Aug;104(4):571–582. doi: 10.1093/genetics/104.4.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Radman M., Villani G., Boiteux S., Kinsella A. R., Glickman B. W., Spadari S. Replicational fidelity: mechanisms of mutation avoidance and mutation fixation. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):937–946. doi: 10.1101/sqb.1979.043.01.103. [DOI] [PubMed] [Google Scholar]
  19. Razin A., Goren D., Friedman J. Studies on the biological role of DNA methylation: inhibition of methylation and maturation of the bacteriophage phichi174 by nicotinamide. Nucleic Acids Res. 1975 Oct;2(10):1967–1974. doi: 10.1093/nar/2.10.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schendel P. F., Robins P. E. Repair of O6-methylguanine in adapted Escherichia coli. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6017–6020. doi: 10.1073/pnas.75.12.6017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Szyf M., Gruenbaum Y., Urieli-Shoval S., Razin A. Studies on the biological role of DNA methylation: V. The pattern of E.coli DNA methylation. Nucleic Acids Res. 1982 Nov 25;10(22):7247–7259. doi: 10.1093/nar/10.22.7247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tye B. K., Nyman P. O., Lehman I. R., Hochhauser S., Weiss B. Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA. Proc Natl Acad Sci U S A. 1977 Jan;74(1):154–157. doi: 10.1073/pnas.74.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wagner R., Jr, Meselson M. Repair tracts in mismatched DNA heteroduplexes. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4135–4139. doi: 10.1073/pnas.73.11.4135. [DOI] [PMC free article] [PubMed] [Google Scholar]

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