Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Jul;159(1):424–426. doi: 10.1128/jb.159.1.424-426.1984

Physical mapping of hemolysin plasmid pCW2, which codes for virulence of a nephropathogenic Escherichia coli strain.

C Waalwijk, J de Graaff, D M MacLaren
PMCID: PMC215654  PMID: 6330049

Abstract

The hemolysin plasmid pCW2, which enhances the virulence of Escherichia coli, has been mapped. Comparison of the region coding for hemolysin with other hemolysin determinants reveals differences that could explain differences in the ability to confer virulence.

Full text

PDF
424

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger H., Hacker J., Juarez A., Hughes C., Goebel W. Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli. J Bacteriol. 1982 Dec;152(3):1241–1247. doi: 10.1128/jb.152.3.1241-1247.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Goebel W., Hedgpeth J. Cloning and functional characterization of the plasmid-encoded hemolysin determinant of Escherichia coli. J Bacteriol. 1982 Sep;151(3):1290–1298. doi: 10.1128/jb.151.3.1290-1298.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hacker J., Knapp S., Goebel W. Spontaneous deletions and flanking regions of the chromosomally inherited hemolysin determinant of an Escherichia coli O6 strain. J Bacteriol. 1983 Jun;154(3):1145–1152. doi: 10.1128/jb.154.3.1145-1152.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jorgensen R. A., Rothstein S. J., Reznikoff W. S. A restriction enzyme cleavage map of Tn5 and location of a region encoding neomycin resistance. Mol Gen Genet. 1979;177(1):65–72. doi: 10.1007/BF00267254. [DOI] [PubMed] [Google Scholar]
  5. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Minshew B. H., Jorgensen J., Counts G. W., Falkow S. Association of hemolysin production, hemagglutination of human erythrocytes, and virulence for chicken embryos of extraintestinal Escherichia coli isolates. Infect Immun. 1978 Apr;20(1):50–54. doi: 10.1128/iai.20.1.50-54.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Noegel A., Rdest U., Goebel W. Determination of the functions of hemolytic plasmid pHly152 of Escherichia coli. J Bacteriol. 1981 Jan;145(1):233–247. doi: 10.1128/jb.145.1.233-247.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Noegel A., Rdest U., Springer W., Goebel W. Plasmid cistrons controlling synthesis and excretion of the exotoxin alpha-haemolysin of Escherichia coli. Mol Gen Genet. 1979 Oct 1;175(3):343–350. doi: 10.1007/BF00397234. [DOI] [PubMed] [Google Scholar]
  9. Stark J. M., Shuster C. W. Analysis of hemolytic determinants of plasmid pHly185 by Tn5 mutagenesis. J Bacteriol. 1982 Nov;152(2):963–967. doi: 10.1128/jb.152.2.963-967.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Stark J. M., Shuster C. W. The structure of cloned hemolysin DNA from plasmid pHly185. Plasmid. 1983 Jul;10(1):45–54. doi: 10.1016/0147-619x(83)90056-2. [DOI] [PubMed] [Google Scholar]
  11. Waalwijk C., de Graaff J. Inactivation of haemolysin production in Escherichia coli by transposon insertion results in loss of virulence. Antonie Van Leeuwenhoek. 1983 Apr;49(1):23–30. doi: 10.1007/BF00457876. [DOI] [PubMed] [Google Scholar]
  12. Welch R. A., Dellinger E. P., Minshew B., Falkow S. Haemolysin contributes to virulence of extra-intestinal E. coli infections. Nature. 1981 Dec 17;294(5842):665–667. doi: 10.1038/294665a0. [DOI] [PubMed] [Google Scholar]
  13. Zabala J. C., de la Cruz F., Ortiz J. M. Relationship of the replication and incompatibility genes of an IncFVI haemolysin plasmid with other F-like haemolysin plasmids. J Gen Microbiol. 1983 Mar;129(3):801–807. doi: 10.1099/00221287-129-3-801. [DOI] [PubMed] [Google Scholar]
  14. Zabala J. C., de la Cruz F., Ortiz J. M. Several copies of the same insertion sequence are present in alpha-hemolytic plasmids belonging to four different incompatibility groups. J Bacteriol. 1982 Jul;151(1):472–476. doi: 10.1128/jb.151.1.472-476.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. de la Cruz F., Müller D., Ortiz J. M., Goebel W. Hemolysis determinant common to Escherichia coli hemolytic plasmids of different incompatibility groups. J Bacteriol. 1980 Aug;143(2):825–833. doi: 10.1128/jb.143.2.825-833.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. van den Bosch J. F., Postma P., Koopman P. A., de Graaff J., MacLaren D. M., van Brenk D. G., Guinée P. A. Virulence of urinary and faecal Escherichia coli in relation to serotype, haemolysis and haemagglutination. J Hyg (Lond) 1982 Jun;88(3):567–577. doi: 10.1017/s002217240007042x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. van den Bosch J. F., Postma P., de Graaff J., MacLaren D. M. Haemolysis by urinary Escherichia coli and virulence in mice. J Med Microbiol. 1981 Aug;14(3):321–331. doi: 10.1099/00222615-14-3-321. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES