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. 1984 Aug;159(2):652–657. doi: 10.1128/jb.159.2.652-657.1984

Spontaneous Nif- mutants of Rhodopseudomonas capsulata.

J D Wall, J Love, S P Quinn
PMCID: PMC215693  PMID: 6146598

Abstract

Revertible, spontaneous Nif- mutants of Rhodopseudomonas capsulata have been shown to accumulate in cultures growing photosynthetically with an amino acid as the nitrogen source such that H2 is maximally produced. The majority of such strains carry mutations which are clustered in a short region of the chromosome, probably representing one or two genes. Because this cluster includes temperature-sensitive mutations, it is also likely that it identifies the structural gene of a polypeptide. The phenotypic characterization of these spontaneous mutants showed (i) an inability to grow with N2 as the nitrogen source, no measurable nitrogenase activity, a reduction or absence of the three polypeptides of the MoFe and Fe proteins of the nitrogenase complex, a faster growth rate on glutamate as the nitrogen source under saturating light, and frequently a small increase in glutamine synthetase activity relative to that of the wild type when grown with glutamate as the nitrogen source. Alterations in other ammonium-assimilatory enzyme activities were not observed. Taken together, these properties suggest that the mutations have affected a regulatory protein necessary for nitrogen fixation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avtges P., Scolnik P. A., Haselkorn R. Genetic and physical map of the structural genes (nifH,D,K) coding for the nitrogenase complex of Rhodopseudomonas capsulata. J Bacteriol. 1983 Oct;156(1):251–256. doi: 10.1128/jb.156.1.251-256.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bender R. A., Janssen K. A., Resnick A. D., Blumenberg M., Foor F., Magasanik B. Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. J Bacteriol. 1977 Feb;129(2):1001–1009. doi: 10.1128/jb.129.2.1001-1009.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GEST H. Oxidation and evolution of molecular hydrogen by microorganisms. Bacteriol Rev. 1954 Mar;18(1):43–73. doi: 10.1128/br.18.1.43-73.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gest H., Kamen M. D. Photoproduction of Molecular Hydrogen by Rhodospirillum rubrum. Science. 1949 Jun 3;109(2840):558–559. doi: 10.1126/science.109.2840.558. [DOI] [PubMed] [Google Scholar]
  5. Hallenbeck P. C., Meyer C. M., Vignais P. M. Nitrogenase from the photosynthetic bacterium Rhodopseudomonas capsulata: purification and molecular properties. J Bacteriol. 1982 Feb;149(2):708–717. doi: 10.1128/jb.149.2.708-717.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hillmer P., Gest H. H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: H2 production by growing cultures. J Bacteriol. 1977 Feb;129(2):724–731. doi: 10.1128/jb.129.2.724-731.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hillmer P., Gest H. H2 metabolism in the photosynthetic bacterium Rhodopseudomonas capsulata: production and utilization of H2 by resting cells. J Bacteriol. 1977 Feb;129(2):732–739. doi: 10.1128/jb.129.2.732-739.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johansson B. C., Gest H. Adenylylation/deadenylylation control of the glutamine synthetase of Rhodopseudomonas capsulata. Eur J Biochem. 1977 Dec 1;81(2):365–371. doi: 10.1111/j.1432-1033.1977.tb11960.x. [DOI] [PubMed] [Google Scholar]
  9. Johansson B. C., Gest H. Inorganic nitrogen assimilation by the photosynthetic bacterium Rhodopseudomonas capsulata. J Bacteriol. 1976 Nov;128(2):683–688. doi: 10.1128/jb.128.2.683-688.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kamen M. D., Gest H. Evidence for a Nitrogenase System in the Photosynthetic Bacterium Rhodospirillum rubrum. Science. 1949 Jun 3;109(2840):560–560. doi: 10.1126/science.109.2840.560. [DOI] [PubMed] [Google Scholar]
  11. Kelley B. C., Nicholas D. J. Inhibition of nitrogenase activity by metronidazole in rhodopseudomonas capsulata. Arch Microbiol. 1981 Jul;129(5):344–348. doi: 10.1007/BF00406459. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Marrs B. Genetic recombination in Rhodopseudomonas capsulata. Proc Natl Acad Sci U S A. 1974 Mar;71(3):971–973. doi: 10.1073/pnas.71.3.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taylor D. P., Cohen S. N., Clark W. G., Marrs B. L. Alignment of genetic and restriction maps of the photosynthesis region of the Rhodopseudomonas capsulata chromosome by a conjugation-mediated marker rescue technique. J Bacteriol. 1983 May;154(2):580–590. doi: 10.1128/jb.154.2.580-590.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wall J. D., Braddock K. Mapping of Rhodopseudomonas capsulata nif genes. J Bacteriol. 1984 May;158(2):404–410. doi: 10.1128/jb.158.2.404-410.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wall J. D., Gest H. Derepression of nitrogenase activity in glutamine auxotrophs of Rhodopseudomonas capsulata. J Bacteriol. 1979 Mar;137(3):1459–1463. doi: 10.1128/jb.137.3.1459-1463.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wall J. D., Johansson B. C., Gest H. A pleiotropic mutant of Rhodopseudomonas capsulata defective in nitrogen metabolism. Arch Microbiol. 1977 Dec 15;115(3):259–263. doi: 10.1007/BF00446450. [DOI] [PubMed] [Google Scholar]
  19. Yen H. C., Hu N. T., Marrs B. L. Characterization of the gene transfer agent made by an overproducer mutant of Rhodopseudomonas capsulata. J Mol Biol. 1979 Jun 25;131(2):157–168. doi: 10.1016/0022-2836(79)90071-8. [DOI] [PubMed] [Google Scholar]
  20. Yen H. C., Marrs B. Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata. J Bacteriol. 1976 May;126(2):619–629. doi: 10.1128/jb.126.2.619-629.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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