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. 1984 Aug;159(2):700–703. doi: 10.1128/jb.159.2.700-703.1984

Development of a minimally defined medium for the acetogen Clostridium thermoaceticum.

L L Lundie Jr, H L Drake
PMCID: PMC215701  PMID: 6746575

Abstract

A minimally defined medium was developed for the cultivation of the acetogen Clostridium thermoaceticum. The medium contained glucose as the carbon and energy source, ammonium sulfate as the nitrogen source, nicotinic acid as the sole essential vitamin, reductant, a phosphate-bicarbonate buffer, mineral salts and chelator, and a CO2 gas phase. Adaptation of C. thermoaceticum from undefined medium containing yeast extract and tryptone to the minimally defined medium required sequential passage on defined medium supplemented with amino acids and vitamins. Growth and cell yields were reduced on the minimal medium, but the activities of carbon monoxide dehydrogenase, hydrogenase, and formate dehydrogenase were comparable between undefined and minimal media.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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