Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Aug;159(2):773–775. doi: 10.1128/jb.159.2.773-775.1984

Evidence that incorporation of exogenous fatty acids into the phospholipids of Escherichia coli does not require acyl carrier protein.

J E Cronan Jr
PMCID: PMC215714  PMID: 6378892

Abstract

Cells of an Escherichia coli acpS mutant were prepared with decreased intracellular concentrations (to 10% of the normal level) of the holo form of acyl carrier protein. These cells incorporated exogenous oleic acid into phospholipid at a normal rate.

Full text

PDF
773

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberts A. W., Vagelos P. R. Acyl carrier protein. 8. Studies of acyl carrier protein and coenzyme A in Escherichia coli pantothenate or betaalanine auxotrophs. J Biol Chem. 1966 Nov 25;241(22):5201–5204. [PubMed] [Google Scholar]
  2. Barnes E. M., Jr Long-chain fatty acyl thioesterases I and II from Escherichia coli. Methods Enzymol. 1975;35:102–109. doi: 10.1016/0076-6879(75)35144-6. [DOI] [PubMed] [Google Scholar]
  3. Cronan J. E., Jr Molecular biology of bacterial membrane lipids. Annu Rev Biochem. 1978;47:163–189. doi: 10.1146/annurev.bi.47.070178.001115. [DOI] [PubMed] [Google Scholar]
  4. Frerman F. E., Bennett W. Studies on the uptake of fatty acids by Escherichia coli. Arch Biochem Biophys. 1973 Nov;159(1):434–443. doi: 10.1016/0003-9861(73)90471-2. [DOI] [PubMed] [Google Scholar]
  5. Greenway D. L., Silbert D. F. Altered acyltransferase activity in Escherichia coli associated with mutations in acyl coenzyme A synthetase. J Biol Chem. 1983 Nov 10;258(21):13034–13042. [PubMed] [Google Scholar]
  6. Jackowski S., Rock C. O. Ratio of active to inactive forms of acyl carrier protein in Escherichia coli. J Biol Chem. 1983 Dec 25;258(24):15186–15191. [PubMed] [Google Scholar]
  7. Jackowski S., Rock C. O. Regulation of coenzyme A biosynthesis. J Bacteriol. 1981 Dec;148(3):926–932. doi: 10.1128/jb.148.3.926-932.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kameda K., Nunn W. D. Purification and characterization of acyl coenzyme A synthetase from Escherichia coli. J Biol Chem. 1981 Jun 10;256(11):5702–5707. [PubMed] [Google Scholar]
  9. Klein K., Steinberg R., Fiethen B., Overath P. Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants. Eur J Biochem. 1971 Apr;19(3):442–450. doi: 10.1111/j.1432-1033.1971.tb01334.x. [DOI] [PubMed] [Google Scholar]
  10. Loewen P. C. Levels of coenzyme A--glutathione mixed disulfide in Escherichia coli. Can J Biochem. 1978 Jul;56(7):753–759. doi: 10.1139/o78-113. [DOI] [PubMed] [Google Scholar]
  11. Maloy S. R., Ginsburgh C. L., Simons R. W., Nunn W. D. Transport of long and medium chain fatty acids by Escherichia coli K12. J Biol Chem. 1981 Apr 25;256(8):3735–3742. [PubMed] [Google Scholar]
  12. Nunn W. D., Simons R. W. Transport of long-chain fatty acids by Escherichia coli: mapping and characterization of mutants in the fadL gene. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3377–3381. doi: 10.1073/pnas.75.7.3377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Polacco M. L., Cronan J. E., Jr A mutant of Escherichia coli conditionally defective in the synthesis of holo-[acyl carrier protein]. J Biol Chem. 1981 Jun 10;256(11):5750–5754. [PubMed] [Google Scholar]
  14. Polacco M. L., Cronan J. E., Jr Mechanism of the apparent regulation of Escherichia coli unsaturated fatty acid synthesis by exogenous oleic acid. J Biol Chem. 1977 Aug 10;252(15):5488–5490. [PubMed] [Google Scholar]
  15. Ray T. K., Cronan J. E., Jr Activation of long chain fatty acids with acyl carrier protein: demonstration of a new enzyme, acyl-acyl carrier protein synthetase, in Escherichia coli. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4374–4378. doi: 10.1073/pnas.73.12.4374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ray T. K., Cronan J. E. Mechanism of phospholipid biosynthesis in Escherichia coli: acyl-CoA synthetase is not required for the incorporation of intracellular free fatty acids into phospholipid. Biochem Biophys Res Commun. 1976 Mar 22;69(2):506–513. doi: 10.1016/0006-291x(76)90550-7. [DOI] [PubMed] [Google Scholar]
  17. Rock C. O., Cronan J. E., Jr Acyl-acyl carrier protein synthetase from Escherichia coli. Methods Enzymol. 1981;71(Pt 100):163–168. doi: 10.1016/0076-6879(81)71023-1. [DOI] [PubMed] [Google Scholar]
  18. Rock C. O., Goelz S. E., Cronan J. E., Jr Phospholipid synthesis in Escherichia coli. Characteristics of fatty acid transfer from acyl-acyl carrier protein to sn-glycerol 3-phosphate. J Biol Chem. 1981 Jan 25;256(2):736–742. [PubMed] [Google Scholar]
  19. Rock C. O., Jackowski S. Regulation of phospholipid synthesis in Escherichia coli. Composition of the acyl-acyl carrier protein pool in vivo. J Biol Chem. 1982 Sep 25;257(18):10759–10765. [PubMed] [Google Scholar]
  20. Samuel D., Estroumza J., Ailhaud G. Partial purification and properties of acyl-CoA synthetase of Escherichia coli. Eur J Biochem. 1970 Feb;12(3):576–582. doi: 10.1111/j.1432-1033.1970.tb00889.x. [DOI] [PubMed] [Google Scholar]
  21. Silbert D. F., Ruch F., Vagelos P. R. Fatty acid replacements in a fatty acid auxotroph of Escherichia coli. J Bacteriol. 1968 May;95(5):1658–1665. doi: 10.1128/jb.95.5.1658-1665.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Spencer A. K., Greenspan A. D., Cronan J. E., Jr Thioesterases I and II of Escherichia coli. Hydrolysis of native acyl-acyl carrier protein thioesters. J Biol Chem. 1978 Sep 10;253(17):5922–5926. [PubMed] [Google Scholar]
  23. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES