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. 1984 Aug;159(2):780–782. doi: 10.1128/jb.159.2.780-782.1984

Cloning of Bordetella pertussis outer membrane proteins in Escherichia coli.

F Shareck, J Cameron
PMCID: PMC215716  PMID: 6086587

Abstract

We constructed and screened a gene bank of phase I chromosomal DNA of Bordetella pertussis in Escherichia coli. A single immunopositive clone was recovered, and the hybrid plasmid obtained, designated pFSH200, had a molecular size of 46.6 kilobases. Smaller derivatives were generated by partial digestion of plasmid pFSH200 and were further characterized. One such derivative, plasmid pFSH201, contained a 4.5-kilobase chromosomal DNA fragment of B. pertussis which coded for the synthesis of the two outer membrane proteins of 33 and 30 kilodaltons specific to B. pertussis.

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Selected References

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