Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Sep;159(3):940–945. doi: 10.1128/jb.159.3.940-945.1984

Dual system for potassium transport in Saccharomyces cerevisiae.

A Rodríguez-Navarro, J Ramos
PMCID: PMC215750  PMID: 6384187

Abstract

In a newly formulated growth medium lacking Na+ and NH4+, Saccharomyces cerevisiae grew maximally at 5 microM K+. Cells grown under these conditions transported K+ with an apparent Km of 24 microM, whereas cells grown in customary high-K+ medium had a significantly higher Km (2 mM K+). The two types of transport also differed in carbonyl cyanide-m-chlorophenyl hydrazone sensitivity, response to ATP depletion, and temperature dependence. The results can be accounted for either by two transport systems or by one system operating in two different ways.

Full text

PDF
940

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARMSTRONG W. M., ROTHSTEIN A. DISCRIMINATION BETWEEN ALKALI METAL CATIONS BY YEAST. I. EFFECT OF PH ON UPTAKE. J Gen Physiol. 1964 Sep;48:61–71. doi: 10.1085/jgp.48.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borst-Pauwels G. W. Ion transport in yeast. Biochim Biophys Acta. 1981 Dec;650(2-3):88–127. doi: 10.1016/0304-4157(81)90002-2. [DOI] [PubMed] [Google Scholar]
  3. CONWAY E. J., DUGGAN F. A cation carrier in the yeast cell wall. Biochem J. 1958 Jun;69(2):265–274. doi: 10.1042/bj0690265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cuppoletti J., Segel I. H. Transinhibition kinetics of the sulfate transport system of Penicillium notatum: analysis based on an iso uni uni velocity equation. J Membr Biol. 1974 Jul 12;17(3):239–252. doi: 10.1007/BF01870185. [DOI] [PubMed] [Google Scholar]
  5. Epstein W., Kim B. S. Potassium transport loci in Escherichia coli K-12. J Bacteriol. 1971 Nov;108(2):639–644. doi: 10.1128/jb.108.2.639-644.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glass A. D. Regulation of potassium absorption in barley roots: an allosteric model. Plant Physiol. 1976 Jul;58(1):33–37. doi: 10.1104/pp.58.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hartwell L. H., McLaughlin C. S. Temperature-sensitive mutants of yeast exhibiting a rapid inhibition of protein synthesis. J Bacteriol. 1968 Nov;96(5):1664–1671. doi: 10.1128/jb.96.5.1664-1671.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heller K. B., Höfer M. Temperature dependence of the energy-linked monosaccharide transport across the cell membrane of Rhodotorula gracilis. J Membr Biol. 1975;21(3-4):261–271. doi: 10.1007/BF01941071. [DOI] [PubMed] [Google Scholar]
  9. Kannan S. Plasmalemma: The Seat of Dual Mechanisms of Ion Absorption in Chlorella pyrenoidosa. Science. 1971 Sep 3;173(4000):927–929. doi: 10.1126/science.173.4000.927. [DOI] [PubMed] [Google Scholar]
  10. Lowendorf H. S., Bazinet G. F., Jr, Slayman C. W. Phosphate transport in Neurospora. Derepression of a high-affinity transport system during phosphorus starvation. Biochim Biophys Acta. 1975 May 21;389(3):541–549. doi: 10.1016/0005-2736(75)90164-9. [DOI] [PubMed] [Google Scholar]
  11. Lowendorf H. S., Slayman C. L., Slayman C. W. Phosphate transport in Neurospora. Kinetic characterization of a constitutive, low-affinity transport system. Biochim Biophys Acta. 1974 Dec 24;373(3):369–382. doi: 10.1016/0005-2736(74)90017-0. [DOI] [PubMed] [Google Scholar]
  12. Rodríguez-Navarro A., Sancho E. D. Cation exchanges of yeast in the absence of magnesium. Biochim Biophys Acta. 1979 Apr 4;552(2):322–330. doi: 10.1016/0005-2736(79)90286-4. [DOI] [PubMed] [Google Scholar]
  13. Ryan J. P., Ryan H. The role of intracellular pH in the regulation of cation exchanges in yeast. Biochem J. 1972 Jun;128(1):139–146. doi: 10.1042/bj1280139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SLAYMAN C. W., TATUM E. L. POTASSIUM TRANSPORT IN NEUROSPORA. I. INTRACELLULAR SODIUM AND POTASSIUM CONCENTRATIONS, AND CATION REQUIREMENTS FOR GROWTH. Biochim Biophys Acta. 1964 Nov 29;88:578–592. [PubMed] [Google Scholar]
  15. Serrano R. Effect of ATPase inhibitors on the proton pump of respiratory-deficient yeast. Eur J Biochem. 1980 Apr;105(2):419–424. doi: 10.1111/j.1432-1033.1980.tb04516.x. [DOI] [PubMed] [Google Scholar]
  16. Slonimski P. P., Perrodin G., Croft J. H. Ethidium bromide induced mutation of yeast mitochondria: complete transformation of cells into respiratory deficient non-chromosomal "petites". Biochem Biophys Res Commun. 1968 Feb 15;30(3):232–239. doi: 10.1016/0006-291x(68)90440-3. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES