Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Sep;159(3):1056–1059. doi: 10.1128/jb.159.3.1056-1059.1984

Excretion of unassembled flagellin by Salmonella typhimurium mutants deficient in hook-associated proteins.

M Homma, H Fujita, S Yamaguchi, T Iino
PMCID: PMC215768  PMID: 6384179

Abstract

Of the flagellar filamentless mutants of Salmonella typhimurium, the flaV, flaU, and flaW mutants, which are defective in hook-associated proteins, synthesized flagellin molecules, but flagella did not polymerize at the tips of the mutant hooks and were excreted into the culture medium as intact monomers.

Full text

PDF
1056

Images in this article

1057Image
on p.1057
1057Image
on p.1057
1057Image
on p.1057

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abram D., Koffler H., Vatter A. E. Basal structure and attachment of flagella in cells of Proteus vulgaris. J Bacteriol. 1965 Nov;90(5):1337–1354. doi: 10.1128/jb.90.5.1337-1354.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Abram D., Vatter A. E., Koffler H. Attachment and structural features of flagella of certain bacilli. J Bacteriol. 1966 May;91(5):2045–2068. doi: 10.1128/jb.91.5.2045-2068.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen-Bazire G., London J. Basal organelles of bacterial flagella. J Bacteriol. 1967 Aug;94(2):458–465. doi: 10.1128/jb.94.2.458-465.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DePamphilis M. L., Adler J. Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cyoplasmic membrane. J Bacteriol. 1971 Jan;105(1):396–407. doi: 10.1128/jb.105.1.396-407.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DePamphilis M. L., Adler J. Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971 Jan;105(1):384–395. doi: 10.1128/jb.105.1.384-395.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Emerson S. U., Tokuyasu K., Simon M. I. Bacterial flagella: polarity of elongation. Science. 1970 Jul 10;169(3941):190–192. doi: 10.1126/science.169.3941.190. [DOI] [PubMed] [Google Scholar]
  7. Fujita H., Yamaguchi S., Taira T., Iino T. A simple method for the isolation of flagellar shape mutants in Salmonella. J Gen Microbiol. 1981 Jul;125(1):213–216. doi: 10.1099/00221287-125-1-213. [DOI] [PubMed] [Google Scholar]
  8. Hoeniger J. F., Van Iterson W., Van Zanten E. N. Basal bodies of bacterial flagella in Proteus mirabilis. II. Electron microscopy of negatively stained material. J Cell Biol. 1966 Dec;31(3):603–618. doi: 10.1083/jcb.31.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Holt S. C., Canale-Parola E. Fine structure of Spirochaeta stenostrepta, a free-living, anaerobic spirochete. J Bacteriol. 1968 Sep;96(3):822–835. doi: 10.1128/jb.96.3.822-835.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Homma M., Kutsukake K., Iino T., Yamaguchi S. Hook-associated proteins essential for flagellar filament formation in Salmonella typhimurium. J Bacteriol. 1984 Jan;157(1):100–108. doi: 10.1128/jb.157.1.100-108.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Howe J. G., Hershey J. W. A sensitive immunoblotting method for measuring protein synthesis initiation factor levels in lysates of Escherichia coli. J Biol Chem. 1981 Dec 25;256(24):12836–12839. [PubMed] [Google Scholar]
  12. Iino T. Assembly of Salmonella flagellin in vitro and in vivo. J Supramol Struct. 1974;2(2-4):372–384. doi: 10.1002/jss.400020226. [DOI] [PubMed] [Google Scholar]
  13. Iino T. Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977;11:161–182. doi: 10.1146/annurev.ge.11.120177.001113. [DOI] [PubMed] [Google Scholar]
  14. Iino T. Polarity of flagellar growth in salmonella. J Gen Microbiol. 1969 May;56(2):227–239. doi: 10.1099/00221287-56-2-227. [DOI] [PubMed] [Google Scholar]
  15. Ikeda T., Kamiya R., Yamaguchi S. Excretion of flagellin by a short-flagella mutant of Salmonella typhimurium. J Bacteriol. 1983 Jan;153(1):506–510. doi: 10.1128/jb.153.1.506-510.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kagawa H., Nishiyama T., Yamaguchi S. Motility development of Salmonella typhimurium cells with flaV mutations after addition of exogenous flagellin. J Bacteriol. 1983 Jul;155(1):435–437. doi: 10.1128/jb.155.1.435-437.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Komeda Y. Fusions of flagellar operons to lactose genes on a mu lac bacteriophage. J Bacteriol. 1982 Apr;150(1):16–26. doi: 10.1128/jb.150.1.16-26.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Komeda Y., Kutsukake K., Iino T. Definition of additional flagellar genes in Escherichia coli K12. Genetics. 1980 Feb;94(2):277–290. doi: 10.1093/genetics/94.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kutsukake K., Iino T., Komeda Y., Yamaguchi S. Functional homology of fla genes between Salmonella typhimurium and Escherichia coli. Mol Gen Genet. 1980 Apr;178(1):59–67. doi: 10.1007/BF00267213. [DOI] [PubMed] [Google Scholar]
  20. O'Brien E. J., Bennett P. M. Structure of straight flagella from a mutant Salmonella. J Mol Biol. 1972 Sep 14;70(1):133–152. doi: 10.1016/0022-2836(72)90168-4. [DOI] [PubMed] [Google Scholar]
  21. Shirakihara Y., Wakabayashi T. Three-dimensional image reconstruction of straight flagella from a mutant Salmonella typhimurium. J Mol Biol. 1979 Jul 5;131(3):485–507. doi: 10.1016/0022-2836(79)90004-4. [DOI] [PubMed] [Google Scholar]
  22. Silverman M., Simon M. I. Bacterial flagella. Annu Rev Microbiol. 1977;31:397–419. doi: 10.1146/annurev.mi.31.100177.002145. [DOI] [PubMed] [Google Scholar]
  23. Suzuki H., Iino T. Absence of messenger ribonucleic acid specific for flagellin in non-flagellate mutants of Salmonella. J Mol Biol. 1975 Jul 15;95(4):549–556. doi: 10.1016/0022-2836(75)90316-2. [DOI] [PubMed] [Google Scholar]
  24. Suzuki H., Iino T. In vitro synthesis of phase-specific flagellin of Salmonella. J Mol Biol. 1973 Nov 25;81(1):57–70. doi: 10.1016/0022-2836(73)90247-7. [DOI] [PubMed] [Google Scholar]
  25. Suzuki T., Iino T., Horiguchi T., Yamaguchi S. Incomplete flagellar structures in nonflagellate mutants of Salmonella typhimurium. J Bacteriol. 1978 Feb;133(2):904–915. doi: 10.1128/jb.133.2.904-915.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES