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. 1984 Dec;160(3):971–975. doi: 10.1128/jb.160.3.971-975.1984

Isolation of a recombination-deficient mutant of Rhodopseudomonas capsulata.

F J Genthner, J D Wall
PMCID: PMC215804  PMID: 6389519

Abstract

To facilitate genetic analysis in the purple photosynthetic bacterium Rhodopseudomonas capsulata, a recombination-deficient derivative was sought. A UV irradiation-sensitive mutant (FG106F) was isolated after mutagenesis, and two procedures were used to determine the recombinational capacity of the mutant. First, recombinants were not detected after transduction of this derivative by the phage-like vector gene transfer agent. Second, an R-prime plasmid containing appropriately marked genes for photosynthesis was introduced by conjugation, and again no recombinants were observed. Additional phenotypes displayed by the mutant that are characteristic of a defect in recombination were an increased sensitivity to DNA-damaging antibiotics and a tendency to filament.

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Selected References

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