Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Dec;160(3):1017–1021. doi: 10.1128/jb.160.3.1017-1021.1984

Relaxation of supercoiled plasmid DNA by oxidative stresses in Escherichia coli.

H Horiuchi, M Takagi, K Yano
PMCID: PMC215811  PMID: 6389504

Abstract

The relaxation of plasmid DNA was observed after the visible light irradiation of Escherichia coli AB1157 harboring plasmid pBR322 or some other plasmids in the presence of a photosensitizing dye, such as toluidine blue or acridine orange, and molecular oxygen. Treatment of the cells with hydroperoxides, such as tert-butyl hydroperoxide, cumene hydroperoxide, and hydrogen peroxide, also caused the plasmid DNA relaxation in vivo. Relaxation was not observed in these treatments of purified pBR322 DNA in vitro. Plasmid DNA relaxation was also detected after near-UV irradiation. Far-UV irradiation did not induce such relaxation.

Full text

PDF
1017

Images in this article

1018Image
on p.1018
1018Image
on p.1018
1019Image
on p.1019
1019Image
on p.1019
1020Image
on p.1020

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ananthaswamy H. N., Eisenstark A. Repair of hydrogen peroxide-induced single-strand breaks in Escherichia coli deoxyribonucleic acid. J Bacteriol. 1977 Apr;130(1):187–191. doi: 10.1128/jb.130.1.187-191.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bolivar F., Backman K. Plasmids of Escherichia coli as cloning vectors. Methods Enzymol. 1979;68:245–267. doi: 10.1016/0076-6879(79)68018-7. [DOI] [PubMed] [Google Scholar]
  3. Dean F., Krasnow M. A., Otter R., Matzuk M. M., Spengler S. J., Cozzarelli N. R. Escherichia coli type-1 topoisomerases: identification, mechanism, and role in recombination. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):769–777. doi: 10.1101/sqb.1983.047.01.088. [DOI] [PubMed] [Google Scholar]
  4. Demple B., Halbrook J., Linn S. Escherichia coli xth mutants are hypersensitive to hydrogen peroxide. J Bacteriol. 1983 Feb;153(2):1079–1082. doi: 10.1128/jb.153.2.1079-1082.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DiNardo S., Voelkel K. A., Sternglanz R., Reynolds A. E., Wright A. Escherichia coli DNA topoisomerase I mutants have compensatory mutations in DNA gyrase genes. Cell. 1982 Nov;31(1):43–51. doi: 10.1016/0092-8674(82)90403-2. [DOI] [PubMed] [Google Scholar]
  6. Gellert M. DNA topoisomerases. Annu Rev Biochem. 1981;50:879–910. doi: 10.1146/annurev.bi.50.070181.004311. [DOI] [PubMed] [Google Scholar]
  7. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Halliwell B. Biochemical mechanisms accounting for the toxic action of oxygen on living organisms: the key role of superoxide dismutase. Cell Biol Int Rep. 1978 Mar;2(2):113–128. doi: 10.1016/0309-1651(78)90032-2. [DOI] [PubMed] [Google Scholar]
  9. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kahn M., Kolter R., Thomas C., Figurski D., Meyer R., Remaut E., Helinski D. R. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. Methods Enzymol. 1979;68:268–280. doi: 10.1016/0076-6879(79)68019-9. [DOI] [PubMed] [Google Scholar]
  11. Lang D., Mitani M. Simplified quantitative electron microscopy of biopolymers. Biopolymers. 1970;9(3):373–379. doi: 10.1002/bip.1970.360090310. [DOI] [PubMed] [Google Scholar]
  12. Lee P. C., Bochner B. R., Ames B. N. AppppA, heat-shock stress, and cell oxidation. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7496–7500. doi: 10.1073/pnas.80.24.7496. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Levin D. E., Hollstein M., Christman M. F., Schwiers E. A., Ames B. N. A new Salmonella tester strain (TA102) with A X T base pairs at the site of mutation detects oxidative mutagens. Proc Natl Acad Sci U S A. 1982 Dec;79(23):7445–7449. doi: 10.1073/pnas.79.23.7445. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lockshon D., Morris D. R. Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors. Nucleic Acids Res. 1983 May 25;11(10):2999–3017. doi: 10.1093/nar/11.10.2999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Menzel R., Gellert M. Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling. Cell. 1983 Aug;34(1):105–113. doi: 10.1016/0092-8674(83)90140-x. [DOI] [PubMed] [Google Scholar]
  16. Moss S. H., Smith K. C. Membrane damage can be a significant factor in the inactivation of Escherichia coli by near-ultraviolet radiation. Photochem Photobiol. 1981 Feb;33(2):203–210. doi: 10.1111/j.1751-1097.1981.tb05325.x. [DOI] [PubMed] [Google Scholar]
  17. Peters J., Jagger J. Inducible repair of near-UV radiation lethal damage in E. coli. Nature. 1981 Jan 15;289(5794):194–195. doi: 10.1038/289194a0. [DOI] [PubMed] [Google Scholar]
  18. Pruss G. J., Manes S. H., Drlica K. Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes. Cell. 1982 Nov;31(1):35–42. doi: 10.1016/0092-8674(82)90402-0. [DOI] [PubMed] [Google Scholar]
  19. Sammartano L. J., Tuveson R. W. Escherichia coli xthA mutants are sensitive to inactivation by broad-spectrum near-UV (300- to 400-nm) radiation. J Bacteriol. 1983 Nov;156(2):904–906. doi: 10.1128/jb.156.2.904-906.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sternglanz R., DiNardo S., Voelkel K. A., Nishimura Y., Hirota Y., Becherer K., Zumstein L., Wang J. C. Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition. Proc Natl Acad Sci U S A. 1981 May;78(5):2747–2751. doi: 10.1073/pnas.78.5.2747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tabak H. F., Flavell R. A. A method for the recovery of DNA from agarose gels. Nucleic Acids Res. 1978 Jul;5(7):2321–2332. doi: 10.1093/nar/5.7.2321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Turner M. A., Eisenstark A. Near-ultraviolet radiation blocks SOS responses to DNA damage in Escherichia coli. Mol Gen Genet. 1984;193(1):33–37. doi: 10.1007/BF00327410. [DOI] [PubMed] [Google Scholar]
  23. Tuveson R. W. Genetic control of near-UV sensitivity independent of excision deficiency (uvrA6) in Escherichia coli K12. Photochem Photobiol. 1980 Nov;32(5):703–705. doi: 10.1111/j.1751-1097.1980.tb04044.x. [DOI] [PubMed] [Google Scholar]
  24. Tuveson R. W., Jonas R. B. Genetic control of near-UV (300-400 NM) sensitivity independent of the recA gene in strains of Escherichia coli K12. Photochem Photobiol. 1979 Dec;30(6):667–676. doi: 10.1111/j.1751-1097.1979.tb07197.x. [DOI] [PubMed] [Google Scholar]
  25. Wakayama Y., Takagi M., Yano K. Gene responsible for protecting Escherichia coli from sodium dodecyl sulfate and toluidine blue plus light. J Bacteriol. 1984 Aug;159(2):527–532. doi: 10.1128/jb.159.2.527-532.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wakayama Y., Takagi M., Yano K. Photosensitized inactivation of E. coli cells in toluidine blue-light system. Photochem Photobiol. 1980 Nov;32(5):601–605. doi: 10.1111/j.1751-1097.1980.tb04028.x. [DOI] [PubMed] [Google Scholar]
  27. Webb R. B., Brown M. S. Action spectra for oxygen-dependent and independent inactivation of Escherichia coli WP2s from 254 to 460 nm. Photochem Photobiol. 1979 Feb;29(2):407–409. doi: 10.1111/j.1751-1097.1979.tb07068.x. [DOI] [PubMed] [Google Scholar]
  28. Webb R. B. Near-UV mutagenesis: photoreactivation of 365-nm-induced mutational lesions in Escherichia coli WP2s. J Bacteriol. 1978 Feb;133(2):860–866. doi: 10.1128/jb.133.2.860-866.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES