Abstract
In an n-alkane-assimilating Candida sp., transfer from glucose- to n-alkane-containing medium induced changes in the microsomal proteins, and several distinctive polypeptides were demonstrated in the solubilized microsomal fraction derived from n-alkane-grown cells. Long-term-labeling and pulse-labeling experiments in vivo demonstrated the synthesis of the specific microsomal polypeptides. The polypeptides were synthesized as in vitro translation products directed by polyadenylated RNA extracted from n-alkane-grown cells. Two major polypeptides were partially purified from the microsomal fraction from n-alkane-grown cells, and antiserum was prepared in a rabbit. Immunoprecipitation of these two polypeptides was accompanied by an increase in the amount of translatable mRNA. The molecular weights of the polypeptides derived from long-term-labeling, pulse-labeling and in vitro translation experiments appeared to be identical.
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- Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blobel G. Control of intracellular protein traffic. Methods Enzymol. 1983;96:663–682. doi: 10.1016/s0076-6879(83)96056-1. [DOI] [PubMed] [Google Scholar]
- Gallo M., Bertrand J. C., Roche B., Azoulay E. Alkane oxidation in Candida tropicalis. Biochim Biophys Acta. 1973 Mar 8;296(3):624–638. doi: 10.1016/0005-2760(73)90123-9. [DOI] [PubMed] [Google Scholar]
- Gilewicz M., Zacek M., Bertrand J. C., Azoulay E. Hydroxylase regulation in Candida tropicalis grown on alkanes. Can J Microbiol. 1979 Feb;25(2):201–206. doi: 10.1139/m79-031. [DOI] [PubMed] [Google Scholar]
- Herrlich P., Schweiger M. DNA- and RNA-directed synthesis in vitro of phage enzymes. Methods Enzymol. 1974;30:654–668. doi: 10.1016/0076-6879(74)30063-8. [DOI] [PubMed] [Google Scholar]
- Kawamura M., Takagi M., Yano K. Cloning of a LEU gene and an ARS site of Candida maltosa. Gene. 1983 Oct;24(2-3):157–162. doi: 10.1016/0378-1119(83)90075-6. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lebeault J. M., Lode E. T., Coon M. J. Fatty acid and hydrocarbon hydroxylation in yeast: role of cytochrome P-450 in Candida tropicalis. Biochem Biophys Res Commun. 1971 Feb 5;42(3):413–419. doi: 10.1016/0006-291x(71)90386-x. [DOI] [PubMed] [Google Scholar]
- Takagi M., Moriya K., Yano K. Induction of cytochrome P450 in petroleum-assimilating yeast--I. selection of a strain and basic characterization of cytochrome P450 induction in the strain. Cell Mol Biol Incl Cyto Enzymol. 1979;25(5):363–369. [PubMed] [Google Scholar]
- Takagi M., Moriya K., Yano K. Induction of cytochrome P450 in petroleum-assimilating yeast-II. Comparison of protein synthesizing activity in the cells grown on glucose and n-tetradecane. Cell Mol Biol Incl Cyto Enzymol. 1979;25(6):371–375. [PubMed] [Google Scholar]
- Ueda M., Tanaka A., Horikawa S., Numa S., Fukui S. Synthesis in vitro of precursor-type carnitine acetyltransferase with messenger RNA from Candida tropicalis. Eur J Biochem. 1984 Feb 1;138(3):451–457. doi: 10.1111/j.1432-1033.1984.tb07937.x. [DOI] [PubMed] [Google Scholar]
- Yamada T., Tanaka A., Horikawa S., Numa S., Fukui S. Cell-free translation and regulation of Candida tropicalis catalase messenger RNA. Eur J Biochem. 1982 Dec 15;129(2):251–255. doi: 10.1111/j.1432-1033.1982.tb07046.x. [DOI] [PubMed] [Google Scholar]