Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1984 Dec;160(3):1175–1177. doi: 10.1128/jb.160.3.1175-1177.1984

Insertion element IS121 is near proA in the chromosomes of Escherichia coli K-12 strains.

M S Timmons, K Spear, R C Deonier
PMCID: PMC215839  PMID: 6094505

Abstract

Insertion element IS121 was mapped between proA and a previously mapped IS5A element in two F-prime plasmids. Results of hybridizations of IS121 to chromosomal DNA from four other strains suggest that IS121 is normally present at this position in the chromosomes of Escherichia coli K-12 strains.

Full text

PDF
1175

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg C. M., Curtiss R., 3rd Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics. 1967 Jul;56(3):503–525. doi: 10.1093/genetics/56.3.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Buvinger W. E., Lampel K. A., Bojanowski R. J., Riley M. Location and analysis of nucleotide sequences at one end of a putative lac transposon in the Escherichia coli chromosome. J Bacteriol. 1984 Aug;159(2):618–623. doi: 10.1128/jb.159.2.618-623.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hadley R. G., Deonier R. C. Specificity in the formation of delta tra F-prime plasmids. J Bacteriol. 1980 Aug;143(2):680–692. doi: 10.1128/jb.143.2.680-692.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hadley R. G., Hu M., Timmons M., Yun K., Deonier R. C. A partial restriction map of the proA-purE region of the Escherichia coli K12 chromosome. Gene. 1983 May-Jun;22(2-3):281–287. doi: 10.1016/0378-1119(83)90113-0. [DOI] [PubMed] [Google Scholar]
  6. Herbert A. A., Guest J. R. Biochemical and genetic studies with lysine+methionine mutants of Escherichia coli: lipoic acid and alpha-ketoglutarate dehydrogenase-less mutants. J Gen Microbiol. 1968 Oct;53(3):363–381. doi: 10.1099/00221287-53-3-363. [DOI] [PubMed] [Google Scholar]
  7. Hu M., Deonier R. C. Mapping of IS1 elements flanking the argF gene region on the Escherichia coli K-12 chromosome. Mol Gen Genet. 1981;181(2):222–229. doi: 10.1007/BF00268430. [DOI] [PubMed] [Google Scholar]
  8. Lam S., Roth J. R. IS200: a Salmonella-specific insertion sequence. Cell. 1983 Oct;34(3):951–960. doi: 10.1016/0092-8674(83)90552-4. [DOI] [PubMed] [Google Scholar]
  9. Lampel K. A., Riley M. Discontinuity of homology of Escherichia coli and Salmonella typhimurium DNA in the lac region. Mol Gen Genet. 1982;186(1):82–86. doi: 10.1007/BF00422916. [DOI] [PubMed] [Google Scholar]
  10. O'Connor M. B., Malamy M. H. A new insertion sequence, IS121, is found on the Mu dI1 (Ap lac) bacteriophage and the Escherichia coli K-12 chromosome. J Bacteriol. 1983 Nov;156(2):669–679. doi: 10.1128/jb.156.2.669-679.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ohtsubo E., Hsu M. T. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F100, F152, and F8 and mapping of the Escherichia coli chromosomal region fep-supE-gal-attlambda-uvrB. J Bacteriol. 1978 Jun;134(3):778–794. doi: 10.1128/jb.134.3.778-794.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Riley M., O'Reilly C., McConnell D. Physical map of Salmonella typhimurium LT2 DNA in the vicinity of the proA gene. J Bacteriol. 1984 Feb;157(2):655–657. doi: 10.1128/jb.157.2.655-657.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sanderson K. E., Hartman P. E. Linkage map of Salmonella typhimurium, edition V. Microbiol Rev. 1978 Jun;42(2):471–519. doi: 10.1128/mr.42.2.471-519.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Savić D. J., Romac S. P., Ehrlich S. D. Inversion in the lactose region of Escherichia coli K-12: inversion termini map within IS3 elements alpha 3 beta 3 and beta 5 alpha 5. J Bacteriol. 1983 Aug;155(2):943–946. doi: 10.1128/jb.155.2.943-946.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schoner B., Schoner R. G. Distribution of IS5 in bacteria. Gene. 1981 Dec;16(1-3):347–352. doi: 10.1016/0378-1119(81)90093-7. [DOI] [PubMed] [Google Scholar]
  16. Signer E. R., Beckwith J. R., Brenner S. Mapping of suppressor loci in Escherichia coli. J Mol Biol. 1965 Nov;14(1):153–166. doi: 10.1016/s0022-2836(65)80237-6. [DOI] [PubMed] [Google Scholar]
  17. Timmons M. S., Bogardus A. M., Deonier R. C. Mapping of chromosomal IS5 elements that mediate type II F-prime plasmid excision in Escherichia coli K-12. J Bacteriol. 1983 Jan;153(1):395–407. doi: 10.1128/jb.153.1.395-407.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. York M. K., Stodolsky M. Characterization of P1argF derivatives from Escherichia coli K12 transduction. I. IS1 elements flank the argF gene segment. Mol Gen Genet. 1981;181(2):230–240. doi: 10.1007/BF00268431. [DOI] [PubMed] [Google Scholar]
  19. York M. K., Stodolsky M. Characterization of P1argF derivatives from Escherichia coli K12 transduction. II. Role of P1 in specialized transduction of argF. Virology. 1982 Jul 15;120(1):130–145. doi: 10.1016/0042-6822(82)90012-5. [DOI] [PubMed] [Google Scholar]
  20. York M. K., Stodolsky M. Characterization of P1argF derivatives from Escherichia coli K12 transduction. III. P1Cm13argF derivatives. Virology. 1982 Dec;123(2):336–343. doi: 10.1016/0042-6822(82)90267-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES