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. 1985 Jun;162(3):1126–1134. doi: 10.1128/jb.162.3.1126-1134.1985

Fusion of liposomes and chromatophores of Rhodopseudomonas capsulata: effect on photosynthetic energy transfer between B875 and reaction center complexes.

J Y Takemoto, T Schonhardt, J R Golecki, G Drews
PMCID: PMC215893  PMID: 3997775

Abstract

The photosynthetic chromatophore membranes of Rhodopseudomonas capsulata were fused with liposomes to investigate the effects of lipid dilution on energy transfer between the bacteriochlorophyll-protein complexes of this membrane. Phosphatidylcholine-containing liposomes were mixed with chromatophores at pH 6.0 to 6.2, and the mixture was fractionated on discontinuous sucrose gradients into four membrane fractions with lipid-to-protein ratios that varied 11-fold. Freeze-fracture electron microscopy revealed that the fractions contained closed vesicles formed by the fusion of liposomes to chromatophores. Particles with 9-nm diameters on the P fracture faces did not appear to change in size with increasing lipid content, but the number of particles per membrane area decreased proportionally with increases in the lipid-to-protein ratio. The bacteriochlorophyll-to-protein ratios, electrophoretic polypeptide profiles on sodium dodecyl sulfate-polyacrylamide gels, and light-induced absorbance changes at 595 nm caused by photosynthetic reaction centers were not altered by fusion. The relative fluorescence emission intensities due to the B875 light-harvesting complex increased significantly with increasing lipid content, but no increases in fluorescence due to the B800-B850 light-harvesting complex were observed. Electron transport rates, measured as succinate-cytochrome c reductase activities, decreased with increased lipid content. The results indicate an uncoupling of energy transfer between the B875 light-harvesting and reaction center complexes with lipid dilution of the chromatophore membrane.

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Selected References

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  1. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bachmann R. C., Gillies K., Takemoto J. Y. Membrane topography of the photosynthetic reaction center polypeptides of Rhodopseudomonas sphaeroides. Biochemistry. 1981 Aug 4;20(16):4590–4596. doi: 10.1021/bi00519a012. [DOI] [PubMed] [Google Scholar]
  3. Casadio R., Venturoli G., Di Gioia A., Castellani P., Leonardi L., Melandri B. A. Phospholipid-enriched bacterial chromatophores. A system suited to investigate the ubiquinone-mediated interactions of protein complexes in photosynthetic oxidoreduction processes. J Biol Chem. 1984 Jul 25;259(14):9149–9157. [PubMed] [Google Scholar]
  4. Drews G., Oelze J. Organization and differentiation of membranes of phototrophic bacteria. Adv Microb Physiol. 1981;22:1–92. doi: 10.1016/s0065-2911(08)60325-2. [DOI] [PubMed] [Google Scholar]
  5. Feick R., van Grondelle R., Rijgersberg C. P., Drews G. Fluorescence emission by wild-type- and mutant-strains of Rhodopseudomonas capsulata. Biochim Biophys Acta. 1980 Dec 3;593(2):241–253. doi: 10.1016/0005-2728(80)90062-6. [DOI] [PubMed] [Google Scholar]
  6. Golecki J. R., Schumacher A., Drews G. The differentiation of the photosynthetic apparatus and the intracytoplasmic membrane in cells of Rhodopseudomonas capsulata upon variation of light intensity. Eur J Cell Biol. 1980 Dec;23(1):1–5. [PubMed] [Google Scholar]
  7. Golecki J., Drews G., Bühler R. The size and number of intramembrane particles in cells of the photosynthetic bacterium Rhodopseudomonas capsulata studied by freeze-fracture electron microscopy. Cytobiologie. 1979 Feb;18(3):381–389. [PubMed] [Google Scholar]
  8. Heathcote P., Clayton R. K. Reconstituted energy transfer from antenna pigment-protein to reaction centres isolated from Rhodopseudomonas sphaeroides. Biochim Biophys Acta. 1977 Mar 11;459(3):506–515. doi: 10.1016/0005-2728(77)90049-4. [DOI] [PubMed] [Google Scholar]
  9. Hunter C. N., van Grondelle R., Holmes N. G., Jones O. T. The reconstitution of energy transfer in membranes from a bacteriochlorophyll-less mutant of Rhodopseudomonas sphaeroides by addition of light-harvesting and reaction centre pigment-protein complexes. Biochim Biophys Acta. 1979 Dec 6;548(3):458–470. doi: 10.1016/0005-2728(79)90058-6. [DOI] [PubMed] [Google Scholar]
  10. Markwell J. P., Lascelles J. Membrane-bound, pyridine nucleotide-independent L-lactate dehydrogenase of Rhodopseudomonas sphaeroides. J Bacteriol. 1978 Feb;133(2):593–600. doi: 10.1128/jb.133.2.593-600.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  12. Monger T. G., Parson W. W. Singlet-triplet fusion in Rhodopseudomonas sphaeroides chromatophores. A probe of the organization of the photosynthetic apparatus. Biochim Biophys Acta. 1977 Jun 9;460(3):393–407. doi: 10.1016/0005-2728(77)90080-9. [DOI] [PubMed] [Google Scholar]
  13. Nelson B. D., Gellerfors P. Characterization and resolution of complex III from beef heart mitochondria. Methods Enzymol. 1978;53:80–91. doi: 10.1016/s0076-6879(78)53016-4. [DOI] [PubMed] [Google Scholar]
  14. Pradel J., Lavergne J., Moya I. Formation and development of photosynthetic units in repigmenting Rhodopseudomonas sphaeroides wild type and "Phofil" mutant strain. Biochim Biophys Acta. 1978 May 10;502(2):169–182. doi: 10.1016/0005-2728(78)90039-7. [DOI] [PubMed] [Google Scholar]
  15. Rivas E., Reiss-Husson F., le Maire M. Physicochemical properties of detergent-solubilized photochemical reaction centers from two strains of Rhodopseudomonas spheroides. Biochemistry. 1980 Jun 24;19(13):2943–2950. doi: 10.1021/bi00554a020. [DOI] [PubMed] [Google Scholar]
  16. Schneider H., Lemasters J. J., Höchli M., Hackenbrock C. R. Liposome-mitochondrial inner membrane fusion. Lateral diffusion of integral electron transfer components. J Biol Chem. 1980 Apr 25;255(8):3748–3756. [PubMed] [Google Scholar]
  17. Shiozawa J. A., Welte W., Hodapp N., Drews G. Studies on the size and composition of the isolated light-harvesting B800-850 pigment-protein complex of Rhodopseudomonas capsulata. Arch Biochem Biophys. 1982 Feb;213(2):473–485. doi: 10.1016/0003-9861(82)90573-2. [DOI] [PubMed] [Google Scholar]
  18. Siegel C. O., Jordan A. E., Miller K. R. Addition of lipid to the photosynthetic membrane: effects on membrane structure and energy transfer. J Cell Biol. 1981 Oct;91(1):113–125. doi: 10.1083/jcb.91.1.113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Slooten L. Reaction center preparations of Rhodopseudomonas spheroides: energy transfer and structure. Biochim Biophys Acta. 1972 Feb 28;256(2):452–466. doi: 10.1016/0005-2728(72)90074-6. [DOI] [PubMed] [Google Scholar]
  20. Snozzi M., Crofts A. R. Electron transport in chromatophores from Rhodopseudomonas sphaeroides GA fused with liposomes. Biochim Biophys Acta. 1984 Aug 31;766(2):451–463. doi: 10.1016/0005-2728(84)90261-5. [DOI] [PubMed] [Google Scholar]
  21. Varga A. R., Staehelin L. A. Spatial differentiation in photosynthetic and non-photosynthetic membranes of Rhodopseudomonas palustris. J Bacteriol. 1983 Jun;154(3):1414–1430. doi: 10.1128/jb.154.3.1414-1430.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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