Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Jun;162(3):1162–1165. doi: 10.1128/jb.162.3.1162-1165.1985

Regulation of the SOS response analyzed by RecA protein amplification.

P Calsou, B Salles
PMCID: PMC215898  PMID: 3888960

Abstract

A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variations in the inducing effects of successive UV doses were related to modulations of the SOS signal level during SOS induction. A reduction in the level of SOS signal was found after 20 min in the wild-type strain, hypothesized to result from negative control of repair functions. A few DNA repair mutants were tested by the same procedure; the uvrA, recF, and umuC genes were involved in SOS induction control, but we found differences in their respective kinetics of expression. On the contrary, in a recB mutant, only a slight effect was obtained on this control.

Full text

PDF
1162

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armengod M. E. recF-dependent recombination as a SOS function. Biochimie. 1982 Aug-Sep;64(8-9):629–632. doi: 10.1016/s0300-9084(82)80101-6. [DOI] [PubMed] [Google Scholar]
  2. Bachmann B. J. Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972 Dec;36(4):525–557. doi: 10.1128/br.36.4.525-557.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bagg A., Kenyon C. J., Walker G. C. Inducibility of a gene product required for UV and chemical mutagenesis in Escherichia coli. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5749–5753. doi: 10.1073/pnas.78.9.5749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brent R., Ptashne M. The lexA gene product represses its own promoter. Proc Natl Acad Sci U S A. 1980 Apr;77(4):1932–1936. doi: 10.1073/pnas.77.4.1932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bridges B. A., Woodgate R. Mutagenic repair in Escherichia coli. X. The umuC gene product may be required for replication past pyrimidine dimers but not for the coding error in UV-mutagenesis. Mol Gen Genet. 1984;196(2):364–366. doi: 10.1007/BF00328073. [DOI] [PubMed] [Google Scholar]
  6. Clark A. J. Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet. 1973;7:67–86. doi: 10.1146/annurev.ge.07.120173.000435. [DOI] [PubMed] [Google Scholar]
  7. Defais M. Role of the E. coli umuC gene product in the repair of single-stranded DNA phage. Mol Gen Genet. 1983;192(3):509–511. doi: 10.1007/BF00392198. [DOI] [PubMed] [Google Scholar]
  8. Horii Z., Clark A. J. Genetic analysis of the recF pathway to genetic recombination in Escherichia coli K12: isolation and characterization of mutants. J Mol Biol. 1973 Oct 25;80(2):327–344. doi: 10.1016/0022-2836(73)90176-9. [DOI] [PubMed] [Google Scholar]
  9. Kato T., Shinoura Y. Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Mol Gen Genet. 1977 Nov 14;156(2):121–131. doi: 10.1007/BF00283484. [DOI] [PubMed] [Google Scholar]
  10. Little J. W., Mount D. W. The SOS regulatory system of Escherichia coli. Cell. 1982 May;29(1):11–22. doi: 10.1016/0092-8674(82)90085-x. [DOI] [PubMed] [Google Scholar]
  11. Little J. W. The SOS regulatory system: control of its state by the level of RecA protease. J Mol Biol. 1983 Jul 15;167(4):791–808. doi: 10.1016/s0022-2836(83)80111-9. [DOI] [PubMed] [Google Scholar]
  12. Lloyd R. G., Thomas A. On the nature of the RecBC and RecF pathways of conjugal recombination in Escherichia coli. Mol Gen Genet. 1983;190(1):156–161. doi: 10.1007/BF00330339. [DOI] [PubMed] [Google Scholar]
  13. Maenhaut-Michel G., Caillet-Fauquet P. Effect of umuC mutations on targeted and untargeted ultraviolet mutagenesis in bacteriophage lambda. J Mol Biol. 1984 Jul 25;177(1):181–187. doi: 10.1016/0022-2836(84)90064-0. [DOI] [PubMed] [Google Scholar]
  14. Markham B. E., Harper J. E., Mount D. W., Sancar G. B., Sancar A., Rupp W. D., Kenyon C. J., Walker G. C. Analysis of mRNA synthesis following induction of the Escherichia coli SOS system. J Mol Biol. 1984 Sep 15;178(2):237–248. doi: 10.1016/0022-2836(84)90142-6. [DOI] [PubMed] [Google Scholar]
  15. Paoletti C., Salles B., Giacomoni P. An immunoradiometric quantitative assay of Escherichia coli recA protein. Biochimie. 1982 Apr;64(4):239–246. doi: 10.1016/s0300-9084(82)80490-2. [DOI] [PubMed] [Google Scholar]
  16. Quillardet P., Hofnung M. Induction by UV light of the SOS function sfiA in Escherichia coli strains deficient or proficient in excision repair. J Bacteriol. 1984 Jan;157(1):35–38. doi: 10.1128/jb.157.1.35-38.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Salles B., Defais M. Signal of induction of recA protein in E. coli. Mutat Res. 1984 Feb;131(2):53–59. doi: 10.1016/0167-8817(84)90011-7. [DOI] [PubMed] [Google Scholar]
  18. Salles B., Paoletti C. Control of UV induction of recA protein. Proc Natl Acad Sci U S A. 1983 Jan;80(1):65–69. doi: 10.1073/pnas.80.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Templin A., Margossian L., Clark A. J. Suppressibility of recA, recB, and recC mutations by nonsense suppressors. J Bacteriol. 1978 May;134(2):590–596. doi: 10.1128/jb.134.2.590-596.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walker G. C. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev. 1984 Mar;48(1):60–93. doi: 10.1128/mr.48.1.60-93.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Witkin E. M. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev. 1976 Dec;40(4):869–907. doi: 10.1128/br.40.4.869-907.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES