Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Jun;162(3):1238–1243. doi: 10.1128/jb.162.3.1238-1243.1985

Plasmid transduction by Bacillus subtilis bacteriophage SPP1: effects of DNA homology between plasmid and bacteriophage.

I Deichelbohrer, J C Alonso, G Lüder, T A Trautner
PMCID: PMC215909  PMID: 3922945

Abstract

Any SPP1 DNA restriction fragment cloned into Bacillus subtilis plasmid pC194 or pUB110 increased the transduction frequency of the plasmid by SPP1 100- to 1,000-fold over the transduction level of the plasmid alone. This increment was observed irrespective of whether a fragment contained the SPP1 packaging origin (pac). Furthermore, an SPP1 derivative into whose genome pC194 DNA had been integrated transduced pC194 DNA with a greatly enhanced frequency. Transduction enhancement mediated by DNA-DNA homology between plasmid and SPP1 was independent of the extent of homology (size range analyzed, 0.5 to 3.9 kilobases) and the recombination proficiency of donor or recipient.

Full text

PDF
1238

Images in this article

1241Image
on p.1241

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bensi G., Iglesias A., Canosi U., Trautner T. A. Plasmid transformation in Bacillus subtilis. The significance of partial homology between plasmid and recipient cell DNAs. Mol Gen Genet. 1981;184(3):400–404. doi: 10.1007/BF00352512. [DOI] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Canosi U., Iglesias A., Trautner T. A. Plasmid transformation in Bacillus subtilis: effects of insertion of Bacillus subtilis DNA into plasmid pC194. Mol Gen Genet. 1981;181(4):434–440. doi: 10.1007/BF00428732. [DOI] [PubMed] [Google Scholar]
  4. Canosi U., Lüder G., Trautner T. A. SPP1-mediated plasmid transduction. J Virol. 1982 Nov;44(2):431–436. doi: 10.1128/jvi.44.2.431-436.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Canosi U., Morelli G., Trautner T. A. The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis. Mol Gen Genet. 1978 Nov 9;166(3):259–267. doi: 10.1007/BF00267617. [DOI] [PubMed] [Google Scholar]
  6. Deichelbohrer I., Messer W., Trautner T. A. Genome of Bacillus subtilis Bacteriophage SPP1: Structure and Nucleotide Sequence of pac, the Origin of DNA Packaging. J Virol. 1982 Apr;42(1):83–90. doi: 10.1128/jvi.42.1.83-90.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ferrari E., Canosi U., Galizzi A., Mazza G. Studies on transduction process by SPP1 phage. J Gen Virol. 1978 Dec;41(3):563–572. doi: 10.1099/0022-1317-41-3-563. [DOI] [PubMed] [Google Scholar]
  8. Heilmann H., Reeve J. N. Construction and use of SPP1v, a viral cloning vector for Bacillus subtilis. Gene. 1982 Jan;17(1):91–100. doi: 10.1016/0378-1119(82)90104-4. [DOI] [PubMed] [Google Scholar]
  9. Iordănescu S. Recombinant plasmid obtained from two different, compatible staphylococcal plasmids. J Bacteriol. 1975 Nov;124(2):597–601. doi: 10.1128/jb.124.2.597-601.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Jackson E. N., Miller H. I., Adams M. L. EcoRI restriction endonuclease cleavage site map of bacteriophage P22DNA. J Mol Biol. 1978 Jan 25;118(3):347–363. doi: 10.1016/0022-2836(78)90233-4. [DOI] [PubMed] [Google Scholar]
  11. Marrero R., Chiafari F. A., Lovett P. S. SP02 particles mediating transduction of a plasmid containing SP02 cohesive ends. J Bacteriol. 1981 Jul;147(1):1–8. doi: 10.1128/jb.147.1.1-8.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Marrero R., Lovett P. S. Transductional selection of cloned bacteriophage phi 105 and SP02 deoxyribonucleic acids in Bacillus subtilis. J Bacteriol. 1980 Aug;143(2):879–886. doi: 10.1128/jb.143.2.879-886.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mattson T., Van Houwe G., Bolle A., Epstein R. Fate of cloned bacteriophage T4 DNA after phage T4 infection of clone-bearing cells. J Mol Biol. 1983 Oct 25;170(2):343–355. doi: 10.1016/s0022-2836(83)80152-1. [DOI] [PubMed] [Google Scholar]
  14. Mattson T., Van Houwe G., Epstein R. Recombination between bacteriophage T4 and plasmid pBR322 molecules containing cloned T4 DNA. J Mol Biol. 1983 Oct 25;170(2):357–379. doi: 10.1016/s0022-2836(83)80153-3. [DOI] [PubMed] [Google Scholar]
  15. Montenegro M. A., Esche H., Trautner T. A. Induction of mutations in B. subtilis phage SPP1 by growth on host cells carrying a mutator DNA polymerase III. Mol Gen Genet. 1976 Dec 8;149(2):131–134. doi: 10.1007/BF00332880. [DOI] [PubMed] [Google Scholar]
  16. Morelli G., Fisseau C., Behrens B., Trautner T. A., Luh J., Ratcliff S. W., Allison D. P., Ganesan A. T. The genome of B. subtilis phage SSP1: the topology of DNA molecules. Mol Gen Genet. 1979 Jan 10;168(2):153–161. doi: 10.1007/BF00431441. [DOI] [PubMed] [Google Scholar]
  17. Morelli G., Montenegro M. A., Hillenbrandt G., Scherzinger E., Trautner T. A. The genome of B. subtilis phage SPP1: assignment of 5'--3'-polarity to the complementary strands of SPP1 DNA. Mol Gen Genet. 1978 Aug 4;164(1):93–97. doi: 10.1007/BF00267603. [DOI] [PubMed] [Google Scholar]
  18. Orbach M. J., Jackson E. N. Transfer of chimeric plasmids among Salmonella typhimurium strains by P22 transduction. J Bacteriol. 1982 Mar;149(3):985–994. doi: 10.1128/jb.149.3.985-994.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Ratcliff S. W., Luh J., Ganesan A. T., Behrens B., Thompson R., Montenegro M. A., Morelli G., Trautner T. A. The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments. Mol Gen Genet. 1979 Jan 10;168(2):165–172. doi: 10.1007/BF00431442. [DOI] [PubMed] [Google Scholar]
  20. Riva S. C. Asymmetric transcription of B. subtilis phage SPP1 DNA in vitro. Biochem Biophys Res Commun. 1969 Mar 31;34(6):824–830. doi: 10.1016/0006-291x(69)90254-x. [DOI] [PubMed] [Google Scholar]
  21. Rottländer E., Trautner T. A. Genetic and transfection studies with B, subtilis phage SP 50. I. Phage mutants with restricted growth on B. subtilis strain 168. Mol Gen Genet. 1970;108(1):47–60. doi: 10.1007/BF00343184. [DOI] [PubMed] [Google Scholar]
  22. Schmidt C., Schmieger H. Selective transduction of recombinant plasmids with cloned pac sites by Salmonella phage P22. Mol Gen Genet. 1984;196(1):123–128. doi: 10.1007/BF00334103. [DOI] [PubMed] [Google Scholar]
  23. Schmieger H. Packaging signals for phage P22 on the chromosome of Salmonella typhimurium. Mol Gen Genet. 1982;187(3):516–518. doi: 10.1007/BF00332637. [DOI] [PubMed] [Google Scholar]
  24. Schmieger H. pac sites are indispensable for in vivo packaging of DNA by phage P22. Mol Gen Genet. 1984;195(1-2):252–255. doi: 10.1007/BF00332755. [DOI] [PubMed] [Google Scholar]
  25. Shimada K., Umene K., Nakamura T., Takagi Y. Recombination in hybrid ColEl DNAs as analyzed by lambda-mediated transduction. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):991–998. doi: 10.1101/sqb.1979.043.01.108. [DOI] [PubMed] [Google Scholar]
  26. Takahashi H., Saito H. Mechanism of pBR322 transduction mediated by cytosine-substituting T4 bacteriophage. Mol Gen Genet. 1982;186(4):497–500. doi: 10.1007/BF00337955. [DOI] [PubMed] [Google Scholar]
  27. Teifel-Greding J. Transduction of multi-copy plasmid pBR322 by bacteriophage Mu. Mol Gen Genet. 1984;197(1):169–174. doi: 10.1007/BF00327938. [DOI] [PubMed] [Google Scholar]
  28. Tye B. K., Huberman J. A., Botstein D. Non-random circular permutation of phage P22 DNA. J Mol Biol. 1974 Jan 5;85(4):501–528. doi: 10.1016/0022-2836(74)90312-x. [DOI] [PubMed] [Google Scholar]
  29. Yang R., Lis J., Wu R. Elution of DNA from agarose gels after electrophoresis. Methods Enzymol. 1979;68:176–182. doi: 10.1016/0076-6879(79)68012-6. [DOI] [PubMed] [Google Scholar]
  30. Yasbin R. E., Young F. E. Transduction in Bacillus subtilis by bacteriophage SPP1. J Virol. 1974 Dec;14(6):1343–1348. doi: 10.1128/jvi.14.6.1343-1348.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES