Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1985 Jun;162(3):1311–1313. doi: 10.1128/jb.162.3.1311-1313.1985

Photodynamic inactivation and mutagenesis by angelicin (isopsoralen) or thiopyronin (methylene red) in wild-type and repair-deficient strains of bacteriophage T4.

J W Drake
PMCID: PMC215922  PMID: 3997779

Abstract

Photodynamic inactivation of bacteriophage T4 particles, mediated by either angelicin or thiopyronin, is enhanced by defects in the T4 uvsW-uvsX-uvsY postreplication repair system but not by a defect in the denV pyrimidine-dimer-excision system. There was no evidence for functional interactions between the two repair systems. As observed previously with 8-methoxypsoralen, photodynamic mutagenesis with angelicin is abolished by defects in the uvsW-uvsX-uvsY system.

Full text

PDF
1311

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boyle J. M., Symonds N. Radiation-sensitive mutants of T4D. I. T4y: a new radiation-sensitive mutant; effect of the mutation on radiation survival, growth and recombination. Mutat Res. 1969 Nov-Dec;8(3):431–439. doi: 10.1016/0027-5107(69)90060-8. [DOI] [PubMed] [Google Scholar]
  2. Conkling M. A., Drake J. W. Isolation and characterization of conditional alleles of bacteriophage T4 genes uvsX and uvsY. Genetics. 1984 Aug;107(4):505–523. doi: 10.1093/genetics/107.4.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Conkling M. A., Grunau J. A., Drake J. W. Gamma-ray mutagenesis in bacteriophage T4. Genetics. 1976 Apr;82(4):565–575. doi: 10.1093/genetics/82.4.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Drake J. W., McGuire J. Properties of r mutants of bacteriophage T4 photodynamically induced in the presence of thiopyronin and psoralen. J Virol. 1967 Apr;1(2):260–267. doi: 10.1128/jvi.1.2.260-267.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Geissler E. Different sensitivities of T4D and lambda mutants to photodynamic action. Mol Gen Genet. 1970;109(3):264–268. doi: 10.1007/BF00267015. [DOI] [PubMed] [Google Scholar]
  6. Green R. R., Drake J. W. Misrepair mutagenesis in bacteriophage T4. Genetics. 1974 Sep;78(1):81–89. doi: 10.1093/genetics/78.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HARM W. Mutants of phage T4 with increased sensitivity to ultraviolet. Virology. 1963 Jan;19:66–71. doi: 10.1016/0042-6822(63)90025-4. [DOI] [PubMed] [Google Scholar]
  8. Hobot J. A., Villiger W., Escaig J., Maeder M., Ryter A., Kellenberger E. Shape and fine structure of nucleoids observed on sections of ultrarapidly frozen and cryosubstituted bacteria. J Bacteriol. 1985 Jun;162(3):960–971. doi: 10.1128/jb.162.3.960-971.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Suzuki K., Yanagisawa F., Fujita H., Ohkido M. Inactivation of T4 bacteriophage by near ultraviolet light in the presence of 8-methoxypsoralen. J Dermatol. 1978 Feb;5(1):15–19. doi: 10.1111/j.1346-8138.1978.tb01043.x. [DOI] [PubMed] [Google Scholar]
  10. Walker G. C. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev. 1984 Mar;48(1):60–93. doi: 10.1128/mr.48.1.60-93.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Zerler B. R., Wallace S. S. Repair of psoralen plus near ultraviolet light damage in bacteriophage T4. Photochem Photobiol. 1979 Sep;30(3):413–416. doi: 10.1111/j.1751-1097.1979.tb07376.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES