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. 1985 Jun;162(3):1322–1324. doi: 10.1128/jb.162.3.1322-1324.1985

Regulation of nitrogen fixation in Rhodospirillum rubrum grown under dark, fermentative conditions.

J E Schultz, J W Gotto, P F Weaver, D C Yoch
PMCID: PMC215926  PMID: 3922950

Abstract

Rhodospirillum rubrum was shown to grow fermentatively on fructose with N2 as a nitrogen source. The nitrogenase activity of these cells was regulated by the NH4+ switch-off/switch-on mechanism in a manner identical to that for photosynthetically grown cells. In vitro, the inactive nitrogenase Fe protein from fermenting cells was reactivated by an endogenous membrane-bound, Mn2+-dependent activating enzyme that was interchangeable with the activating enzyme isolated from photosynthetic membranes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carithers R. P., Yoch D. C., Arnon D. I. Two forms of nitrogenase from the photosynthetic bacterium Rhodospirillum rubrum. J Bacteriol. 1979 Feb;137(2):779–789. doi: 10.1128/jb.137.2.779-789.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gotto J. W., Yoch D. C. Purification and Mn2+ activation of Rhodospirillum rubrum nitrogenase activating enzyme. J Bacteriol. 1982 Nov;152(2):714–721. doi: 10.1128/jb.152.2.714-721.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gotto J. W., Yoch D. C. Regulation of Rhodospirillum rubrum nitrogenase activity. Properties and interconversion of active and inactive Fe protein. J Biol Chem. 1982 Mar 25;257(6):2868–2873. [PubMed] [Google Scholar]
  4. Gotto J. W., Yoch D. C. Regulation of nitrogenase activity by covalent modification in Chromatium vinosum. Arch Microbiol. 1985 Feb;141(1):40–43. doi: 10.1007/BF00446737. [DOI] [PubMed] [Google Scholar]
  5. Kanemoto R. H., Ludden P. W. Effect of ammonia, darkness, and phenazine methosulfate on whole-cell nitrogenase activity and Fe protein modification in Rhodospirillum rubrum. J Bacteriol. 1984 May;158(2):713–720. doi: 10.1128/jb.158.2.713-720.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ludden P. W., Burris R. H. Activating factor for the iron protein of nitrogenase from Rhodospirillum rubrum. Science. 1976 Oct 22;194(4263):424–426. doi: 10.1126/science.824729. [DOI] [PubMed] [Google Scholar]
  7. Ludden P. W., Burris R. H. Removal of an adenine-like molecule during activation of dinitrogenase reductase from Rhodospirillum rubrum. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6201–6205. doi: 10.1073/pnas.76.12.6201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Madigan M. T., Wall J. D., Gest H. Dark anaerobic dinitrogen fixation by a photosynthetic microorganism. Science. 1979 Jun 29;204(4400):1429–1430. doi: 10.1126/science.204.4400.1429. [DOI] [PubMed] [Google Scholar]
  9. Neilson A. H., Nordlund S. Regulation of nitrogenase synthesis in intact cells of Rhodospirillum rubrum: inactivation of nitrogen fixation by ammonia, L-glutamine and L-asparagine. J Gen Microbiol. 1975 Nov;91(1):53–62. doi: 10.1099/00221287-91-1-53. [DOI] [PubMed] [Google Scholar]
  10. Nordlund S., Eriksson U. Nitrogenase from Rhodospirillum rubrum. Relation between 'switch-off' effect and the membrane component. Hydrogen production and acetylene reduction with different nitrogenase component ratios. Biochim Biophys Acta. 1979 Sep 11;547(3):429–437. doi: 10.1016/0005-2728(79)90023-9. [DOI] [PubMed] [Google Scholar]
  11. Nordlund S., Ludden P. W. Incorporation of adenine into the modifying group of inactive iron protein of nitrogenase from Rhodospirillum rubrum. Biochem J. 1983 Mar 1;209(3):881–884. doi: 10.1042/bj2090881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ORMEROD J. G., ORMEROD K. S., GEST H. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys. 1961 Sep;94:449–463. doi: 10.1016/0003-9861(61)90073-x. [DOI] [PubMed] [Google Scholar]
  13. Schick H. J. Substrate and light dependent fixation of molecular nitrogen in Rhodospirillum rubrum. Arch Mikrobiol. 1971;75(2):89–101. doi: 10.1007/BF00407997. [DOI] [PubMed] [Google Scholar]
  14. Schultz J. E., Weaver P. F. Fermentation and anaerobic respiration by Rhodospirillum rubrum and Rhodopseudomonas capsulata. J Bacteriol. 1982 Jan;149(1):181–190. doi: 10.1128/jb.149.1.181-190.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yoch D. C. Manganese, an essential trace element for N2 fixation by Rhodospirillum rubrum and Rhodopseudomonas capsulata: role in nitrogenase regulation. J Bacteriol. 1979 Dec;140(3):987–995. doi: 10.1128/jb.140.3.987-995.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zumft W. G., Castillo F. Regulatory properties of the nitrogenase from Rhodopseudomonas palustris. Arch Microbiol. 1978 Apr 27;117(1):53–60. doi: 10.1007/BF00689351. [DOI] [PubMed] [Google Scholar]

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