Abstract
The cell wall degradation products released from Escherichia coli during autolysis triggered by cephaloridine or trichloroacetic acid were isolated and characterized. Murein was selectively lost from the disaccharide tetrapeptides and the bisdisaccharide tetrapeptide components. Two major autolytic products accounted for more than 85% of the released material. Compound 1 (60 to 80% of released material) was a disaccharide tetrapeptide monomer containing a 1,6-anhydromuramic acid residue. Compound 2 (15 to 30% of released material) was a mixture of a tritripeptide and a tritetrapeptide without hexosamines. Taken together the findings suggest that autolytic cell wall degradation in E. coli is selective and involves the activity of both the hydrolytic transglycosylase and an endopeptidase. Upon release, at least some of the wall components were also exposed to the activity of the N-acetylmuramic acid-L-alanine amidase.
Full text
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Curtiss R., 3rd Biological containment and cloning vector transmissibility. J Infect Dis. 1978 May;137(5):668–675. doi: 10.1093/infdis/137.5.668. [DOI] [PubMed] [Google Scholar]
- Dougherty T. J. Analysis of Neisseria gonorrhoeae peptidoglycan by reverse-phase, high-pressure liquid chromatography. J Bacteriol. 1985 Jul;163(1):69–74. doi: 10.1128/jb.163.1.69-74.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodell E. W., Schwarz U. Release of cell wall peptides into culture medium by exponentially growing Escherichia coli. J Bacteriol. 1985 Apr;162(1):391–397. doi: 10.1128/jb.162.1.391-397.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goodell W., Tomasz A. Alteration of Escherichia coli murein during amino acid starvation. J Bacteriol. 1980 Dec;144(3):1009–1016. doi: 10.1128/jb.144.3.1009-1016.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hakenbeck R., Messer W. Activity of murein hydrolases in synchronized cultures of Escherichia coli. J Bacteriol. 1977 Mar;129(3):1239–1244. doi: 10.1128/jb.129.3.1239-1244.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartmann R., Bock-Hennig S. B., Schwarz U. Murein hydrolases in the envelope of Escherichia coli. Properties in situ and solubilization from the envelope. Eur J Biochem. 1974 Jan 3;41(1):203–208. doi: 10.1111/j.1432-1033.1974.tb03261.x. [DOI] [PubMed] [Google Scholar]
- Höltje J. V., Mirelman D., Sharon N., Schwarz U. Novel type of murein transglycosylase in Escherichia coli. J Bacteriol. 1975 Dec;124(3):1067–1076. doi: 10.1128/jb.124.3.1067-1076.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Imada A., Nozaki Y., Kawashima F., Yoneda M. Regulation of glucosamine utilization in Staphylococcus aureus and Escherichia coli. J Gen Microbiol. 1977 Jun;100(2):329–337. doi: 10.1099/00221287-100-2-329. [DOI] [PubMed] [Google Scholar]
- Kitano K., Tomasz A. Escherichia coli mutants tolerant to beta-lactam antibiotics. J Bacteriol. 1979 Dec;140(3):955–963. doi: 10.1128/jb.140.3.955-963.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pisabarro A. G., de Pedro M. A., Vázquez D. Structural modifications in the peptidoglycan of Escherichia coli associated with changes in the state of growth of the culture. J Bacteriol. 1985 Jan;161(1):238–242. doi: 10.1128/jb.161.1.238-242.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryter A., Hirota Y., Schwarz U. Process of cellular division in Escherichia coli growth pattern of E. coli murein. J Mol Biol. 1973 Jun 25;78(1):185–195. doi: 10.1016/0022-2836(73)90437-3. [DOI] [PubMed] [Google Scholar]
- van Heijenoort J., Parquet C., Flouret B., van Heijenoort Y. Envelope-bound N-acetylmuramyl-L-alanine amidase of Escherichia coli K 12. Purification and properties of the enzyme. Eur J Biochem. 1975 Oct 15;58(2):611–619. doi: 10.1111/j.1432-1033.1975.tb02412.x. [DOI] [PubMed] [Google Scholar]