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. 1986 Sep;167(3):805–808. doi: 10.1128/jb.167.3.805-808.1986

Acquisition of glucose by Rickettsia prowazekii through the nucleotide intermediate uridine 5'-diphosphoglucose.

H H Winkler, R M Daugherty
PMCID: PMC215945  PMID: 3091580

Abstract

The ability of Rickettsia prowazekii to transport potential sources of the glucose moiety of bacterial polysaccharides was determined. Transport was determined both by filtration assays and by centrifugation through nonaqueous layers. Uridine 5'-diphosphoglucose (UDPG) was transported, whereas glucose was not transported; the uptake of glucose phosphates, although greater than that for glucose, was markedly lower than the transport of UDPG. Furthermore, the activities of hexokinase and phosphoglucomutase, enzymes required for the metabolism of glucose and glucose 6-phosphate, were undetectable in rickettsial extracts. The uptake of UDPG had an extended time course and did not reach a plateau until 60 min. The maximum rate of uptake was 340 pmol/min per mg of protein, and the rate was half-maximal at a UDPG concentration of 220 microM. Measurement of true influx of UDPG was complicated by the low activity of this transport system and the metabolism of the UDPG. The uptake of labeled UDPG was markedly inhibited by a 10-fold excess of uridine monophosphate, uridine diphospho-N-acetylglucosamine, and uridine diphospho-N-acetylgalactosamine but not by a variety of other structurally related compounds.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anacker R. L., List R. H., Mann R. E., Wiedbrauk D. L. Antigenic heterogeneity in high- and low-virulence strains of Rickettsia rickettsii revealed by monoclonal antibodies. Infect Immun. 1986 Feb;51(2):653–660. doi: 10.1128/iai.51.2.653-660.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anacker R. L., Pickens E. G., Lackman D. B. Details of the ultrastructure of Rickettsia prowazekii grown in the chick yolk sac. J Bacteriol. 1967 Jul;94(1):260–262. doi: 10.1128/jb.94.1.260-262.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BOVARNICK M. R. Phosphorylation accompanying the oxidation of glutamate by the Madrid E strain of typhus rickettsiae. J Biol Chem. 1956 May;220(1):353–361. [PubMed] [Google Scholar]
  5. Coolbaugh J. C., Progar J. J., Weiss E. Enzymatic activities of cell-free extracts of Rickettsia typhi. Infect Immun. 1976 Jul;14(1):298–305. doi: 10.1128/iai.14.1.298-305.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fukui S. Active transport of uridine diphosphate glucose in Agrobacterium tumefaciens. J Biochem. 1969 Dec;66(6):873–876. doi: 10.1093/oxfordjournals.jbchem.a129220. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Moulder J. W. Comparative biology of intracellular parasitism. Microbiol Rev. 1985 Sep;49(3):298–337. doi: 10.1128/mr.49.3.298-337.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Schramek S., Brezina R., Kazár J. Some biological properties of an endotoxic lipopolysaccharide from the typhus group rickettsiae. Acta Virol. 1977 Sep;21(5):439–441. [PubMed] [Google Scholar]
  10. Silverman D. J., Wisseman C. L., Jr, Waddell A. D., Jones M. External layers of Rickettsia prowazekii and Rickettsia rickettsii: occurrence of a slime layer. Infect Immun. 1978 Oct;22(1):233–246. doi: 10.1128/iai.22.1.233-246.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith D. K., Winkler H. H. Characterization of a lysine-specific active transport system in Rickettsia prowazeki. J Bacteriol. 1977 Mar;129(3):1349–1355. doi: 10.1128/jb.129.3.1349-1355.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith D. K., Winkler H. H. Separation of inner and outer membranes of Rickettsia prowazeki and characterization of their polypeptide compositions. J Bacteriol. 1979 Feb;137(2):963–971. doi: 10.1128/jb.137.2.963-971.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Walker T. S., Winkler H. H. Rickettsial hemolysis: rapid method for enumeration of metabolically active typhus rickettsiae. J Clin Microbiol. 1979 May;9(5):645–647. doi: 10.1128/jcm.9.5.645-647.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weiss E., Coolbaugh J. C., Williams J. C. Separation of viable Rickettsia typhi from yolk sac and L cell host components by renografin density gradient centrifugation. Appl Microbiol. 1975 Sep;30(3):456–463. doi: 10.1128/am.30.3.456-463.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Williams J. C., Weiss E. Energy metabolism of Rickettsia typhi: pools of adenine nucleotides and energy charge in the presence and absence of glutamate. J Bacteriol. 1978 Jun;134(3):884–892. doi: 10.1128/jb.134.3.884-892.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Winkler H. H. Rickettsial permeability. An ADP-ATP transport system. J Biol Chem. 1976 Jan 25;251(2):389–396. [PubMed] [Google Scholar]
  17. Wisseman C. L., Jr, Waddell A. D. In vitro studies on rickettsia-host cell interactions: intracellular growth cycle of virulent and attenuated Rickettsia prowazeki in chicken embryo cells in slide chamber cultures. Infect Immun. 1975 Jun;11(6):1391–1404. doi: 10.1128/iai.11.6.1391-1401.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wood W. H., Jr, Wisseman C. L., Jr The cell wall of Rickettsia mooseri. I. Morphology and chemical composition. J Bacteriol. 1967 Mar;93(3):1113–1118. doi: 10.1128/jb.93.3.1113-1118.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zhivkov V. I., Tosheva R. T. Uridine diphosphate sugars: concentration and rate of synthesis in tissues of vertebrates. Int J Biochem. 1986;18(1):1–6. doi: 10.1016/0020-711x(86)90002-9. [DOI] [PubMed] [Google Scholar]

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