Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 Sep;167(3):1071–1073. doi: 10.1128/jb.167.3.1071-1073.1986

Cloning and expression of the ltf gene of bacteriophage T5.

K J Heller, V Krauel
PMCID: PMC215983  PMID: 2943727

Abstract

The unique 5-kilobase BamHI fragment of bacteriophage T5 was cloned into plasmid pBR322. Location of the intact ltf gene on the cloned fragment was demonstrated by complementation of the ltf mutation of phage T5hd-2, identification of a plasmid-coded polypeptide of the same molecular weight as the polypeptide forming the L-shaped tail fibers, which binds to anti-T5 antibodies; and analyses of transposon Tn1000 insertions.

Full text

PDF
1071

Images in this article

1072Image
on p.1072
1073Image
on p.1073

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun V., Schaller K., Wolff H. A common receptor protein for phage T5 and colicin M in the outer membrane of Escherichia coli B. Biochim Biophys Acta. 1973 Sep 27;323(1):87–97. doi: 10.1016/0005-2736(73)90433-1. [DOI] [PubMed] [Google Scholar]
  3. Brunel F., Thi V. H., Pilaete M. F., Davison J. Transcription regulatory elements in the late region of bacteriophage T5 DNA. Nucleic Acids Res. 1983 Nov 11;11(21):7649–7658. doi: 10.1093/nar/11.21.7649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hantke K. Identification of an iron uptake system specific for coprogen and rhodotorulic acid in Escherichia coli K12. Mol Gen Genet. 1983;191(2):301–306. doi: 10.1007/BF00334830. [DOI] [PubMed] [Google Scholar]
  5. Heller K. J. Identification of the phage gene for host receptor specificity by analyzing hybrid phages of T5 and BF23. Virology. 1984 Nov;139(1):11–21. doi: 10.1016/0042-6822(84)90325-8. [DOI] [PubMed] [Google Scholar]
  6. Heller K., Braun V. Accelerated adsorption of bacteriophage T5 to Escherichia coli F, resulting from reversible tail fiber-lipopolysaccharide binding. J Bacteriol. 1979 Jul;139(1):32–38. doi: 10.1128/jb.139.1.32-38.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heller K., Braun V. Polymannose O-antigens of Escherichia coli, the binding sites for the reversible adsorption of bacteriophage T5+ via the L-shaped tail fibers. J Virol. 1982 Jan;41(1):222–227. doi: 10.1128/jvi.41.1.222-227.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Heller K., Mann B. J., Kadner R. J. Cloning and expression of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli. J Bacteriol. 1985 Mar;161(3):896–903. doi: 10.1128/jb.161.3.896-903.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hendrickson H. E., Bujard H. Structure and function of the genome of coliphage T5. 2. Regions of transcription of the chromosome. Eur J Biochem. 1973 Mar 15;33(3):529–534. doi: 10.1111/j.1432-1033.1973.tb02712.x. [DOI] [PubMed] [Google Scholar]
  10. Hendrickson H. E., McCorquodale D. J. Genetic and physiological studies of bacteriophage t5 I. An expanded genetic map of t5. J Virol. 1971 May;7(5):612–618. doi: 10.1128/jvi.7.5.612-618.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LANNI Y. T. Lysis inhibition with a mutant of bacteriophage T5. Virology. 1958 Jun;5(3):481–501. doi: 10.1016/0042-6822(58)90041-2. [DOI] [PubMed] [Google Scholar]
  12. Lugtenberg B., Meijers J., Peters R., van der Hoek P., van Alphen L. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands. FEBS Lett. 1975 Oct 15;58(1):254–258. doi: 10.1016/0014-5793(75)80272-9. [DOI] [PubMed] [Google Scholar]
  13. McCorquodale D. J. The T-odd bacteriophages. CRC Crit Rev Microbiol. 1975 Dec;4(2):101–159. doi: 10.3109/10408417509111574. [DOI] [PubMed] [Google Scholar]
  14. Rhoades M. New physical map of bacteriophage T5 DNA. J Virol. 1982 Aug;43(2):566–573. doi: 10.1128/jvi.43.2.566-573.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Saigo K. Isolation of high-density mutants and identification of nonessential structural proteins in bacteriophage T5; dispensability of L-shaped tail fibers and a secondary major head protein. Virology. 1978 Apr;85(2):422–433. doi: 10.1016/0042-6822(78)90449-x. [DOI] [PubMed] [Google Scholar]
  16. Zweig M., Cummings D. J. Cleavage of head and tail proteins during bacteriophage T5 assembly: selective host involvement in the cleavage of a tail protein. J Mol Biol. 1973 Nov 5;80(3):505–518. doi: 10.1016/0022-2836(73)90418-x. [DOI] [PubMed] [Google Scholar]
  17. von Gabain A., Bujard H. Interaction of Escherichia coli RNA polymerase with promoters of several coliphage and plasmid DNAs. Proc Natl Acad Sci U S A. 1979 Jan;76(1):189–193. doi: 10.1073/pnas.76.1.189. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES