Abstract
Data from previous studies of Rhizobium meliloti mutants have been consistent with the catabolism of hexoses via the Entner-Doudoroff pathway. However, galactose metabolism was not impaired in those mutants. We show here by enzymatic assay and by identification of a galactose mutant lacking 2-keto-3-deoxy-6-phosphogalactonate aldolase that the De Ley-Doudoroff pathway is used for galactose metabolism. Mutants in this pathway have not been previously reported for any organism.
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- Arias A., Cerveńansky C., Gardiol A., Martínez-Drets G. Phosphoglucose isomerase mutant of Rhizobium meliloti. J Bacteriol. 1979 Jan;137(1):409–414. doi: 10.1128/jb.137.1.409-414.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cerveñanský C., Arias A. Glucose-6-phosphate dehydrogenase deficiency in pleiotropic carbohydrate-negative mutant strains of Rhizobium meliloti. J Bacteriol. 1984 Dec;160(3):1027–1030. doi: 10.1128/jb.160.3.1027-1030.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE LEY J., DOUDOROFF M. The metabolism of D-galactose in Pseudomonas saccharophila. J Biol Chem. 1957 Aug;227(2):745–757. [PubMed] [Google Scholar]
- Dahms A. S., Anderson R. L. D-Fucose metabolism in a pseudomonad. II. Oxidation of D-fucose to D-fucono- -lactone by an L-arabino-aldose dehydrogenase and hydrolysis of the lactone by a lactonase. J Biol Chem. 1972 Apr 10;247(7):2228–2232. [PubMed] [Google Scholar]
- Dahms A. S., Sibley D., Huisman W., Donald A. D-Galactonate dehydratase. Methods Enzymol. 1982;90(Pt E):294–298. doi: 10.1016/s0076-6879(82)90143-4. [DOI] [PubMed] [Google Scholar]
- Duncan M. J., Fraenkel D. G. alpha-Ketoglutarate dehydrogenase mutant of Rhizobium meliloti. J Bacteriol. 1979 Jan;137(1):415–419. doi: 10.1128/jb.137.1.415-419.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lessie T. G., Phibbs P. V., Jr Alternative pathways of carbohydrate utilization in pseudomonads. Annu Rev Microbiol. 1984;38:359–388. doi: 10.1146/annurev.mi.38.100184.002043. [DOI] [PubMed] [Google Scholar]
- MACGEE J., DOUDOROFF M. A new phosphorylated intermediate in glucose oxidation. J Biol Chem. 1954 Oct;210(2):617–626. [PubMed] [Google Scholar]
- Meloche H. P., O'Connell E. L. 2-Keto-3-deoxy-galactonate-6-phosphate aldolase from Pseudomonas saccharophila. Methods Enzymol. 1982;90(Pt E):263–269. doi: 10.1016/s0076-6879(82)90137-9. [DOI] [PubMed] [Google Scholar]