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. 1981 Jul;147(1):186–192. doi: 10.1128/jb.147.1.186-192.1981

Release of colicin E2 from Escherichia coli.

A P Pugsley, J P Rosenbusch
PMCID: PMC216024  PMID: 6787024

Abstract

Treatment of Escherichia coli K-12(ColE2.P9) with 500 ng of mitomycin C per ml resulted in rapid and almost synchronous colicin E2 production. Colicin accumulated outside the cytoplasmic membrane, most probably in the periplasmic space. Colicin release occurred during a period in which the turbidity of the culture declined markedly. Periplasmic alkaline phosphatase was released during the same period, but cytoplasmic beta-galactosidase release was delayed.

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Selected References

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