Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Jul;147(1):262–266. doi: 10.1128/jb.147.1.262-266.1981

Isolation of a plasmid responsible for caseinase activity in Clostridium perfringens ATCC 3626B.

H P Blaschek, M Solberg
PMCID: PMC216034  PMID: 6263868

Abstract

Clostridium perfringens strain ATCC 3626B was cured of caseinase activity at a high frequency after treatment with acriflavine dye (2.5%) or elevated temperature growth (9.1%). Caseinase-negative isolates retained the larger (9.4 megadaltons) pHB102 cryptic plasmid, but were missing the smaller (2.1 megadaltons) pHB101 plasmid present in the caseinase-positive wild-type strain. Dye-buoyant density-gradient centrifugation at 4 or 15 degrees C revealed that the pHB101 and pHB102 plasmids are temperature labile and easily converted into the nicked non-supercoiled or linear state.

Full text

PDF
262

Images in this article

263Image
on p.263
264Image
on p.264

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaij C., Borst P. The gel electrophoresis of DNA. Biochim Biophys Acta. 1972 May 10;269(2):192–200. doi: 10.1016/0005-2787(72)90426-1. [DOI] [PubMed] [Google Scholar]
  2. BIDWELL E. Proteolytic enzymes of Clostridium welchii. Biochem J. 1950 May;46(5):589–598. doi: 10.1042/bj0460589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brefort G., Magot M., Ionesco H., Sebald M. Characterization and transferability of Clostridium perfringens plasmids. Plasmid. 1977 Nov;1(1):52–66. doi: 10.1016/0147-619x(77)90008-7. [DOI] [PubMed] [Google Scholar]
  4. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Inman R. B., Schnös M. Partial denaturation of thymine- and 5-bromouracil-containing lambda DNA in alkali. J Mol Biol. 1970 Apr 14;49(1):93–98. doi: 10.1016/0022-2836(70)90378-5. [DOI] [PubMed] [Google Scholar]
  8. Ionesco H., Bieth G., Dauguet C., Bouanchaud D. Isolement et identification de deux plasmides d'une souche bactériocinogène de Clostridium perfringens. Ann Microbiol (Paris) 1976 Oct;127B(3):283–294. [PubMed] [Google Scholar]
  9. Ionesco H., Bouanchaud D. H. Production de bactériocine liée à présence d'un plasmide chez Clostridium perfringens type. C R Acad Sci Hebd Seances Acad Sci D. 1973 May 14;276(20):2855–2857. [PubMed] [Google Scholar]
  10. Ionesco H., Wolff A., Sebald Production induite de bactériocine et d'un bactériophage par la souche BP6K-N-5 de Clostridium perfringens. Ann Microbiol (Paris) 1974 Oct-Nov;125B(3):335–346. [PubMed] [Google Scholar]
  11. Lang D. Molecular weights of coliphages and coliphage DNA. 3. Contour length and molecular weight of DNA from bacteriophages T4, T5 and T7, and from bovine papilloma virus. J Mol Biol. 1970 Dec 28;54(3):557–565. doi: 10.1016/0022-2836(70)90126-9. [DOI] [PubMed] [Google Scholar]
  12. Li A. W., Krell P. J., Mahony D. E. Plasmid detection in a bacteriocinogenic strain of Clostridium perfringens. Can J Microbiol. 1980 Aug;26(8):1018–1022. doi: 10.1139/m80-173. [DOI] [PubMed] [Google Scholar]
  13. Martley F. G., Jayashankar S. R., Lawrence R. C. An improved agar medium for the detection of proteolytic organisms in total bacterial counts. J Appl Bacteriol. 1970 Jun;33(2):363–370. doi: 10.1111/j.1365-2672.1970.tb02208.x. [DOI] [PubMed] [Google Scholar]
  14. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. OAKLEY C. L., WARRACK G. H. Routine typing of Clostridium welchii. J Hyg (Lond) 1953 Mar;51(1):102–107. doi: 10.1017/s0022172400015527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pearce L. E., Skipper N. A., Jarvis B. D. Proteinase activity in slow lactic acid-producing variants of Streptococcus lactis. Appl Microbiol. 1974 May;27(5):933–937. doi: 10.1128/am.27.5.933-937.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rood J. I., Maher E. A., Somers E. B., Campos E., Duncan C. L. Isolation and characterization of multiply antibiotic-resistant Clostridum perfringens strains from porcine feces. Antimicrob Agents Chemother. 1978 May;13(5):871–880. doi: 10.1128/aac.13.5.871. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rood J. I., Scott V. N., Duncan C. L. Identification of a transferable tetracycline resistance plasmid (pCW3) from Clostridium perfringens. Plasmid. 1978 Sep;1(4):563–570. doi: 10.1016/0147-619x(78)90013-6. [DOI] [PubMed] [Google Scholar]
  20. Sebald M., Bréfort M. G. Transfert du plasmide Tétracycline-Chloramphénicol chez Clostridium perfringens. C R Acad Sci Hebd Seances Acad Sci D. 1975 Jul 28;281(4):317–319. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES