Skip to main content
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Aug;147(2):282–288. doi: 10.1128/jb.147.2.282-288.1981

Immunological comparison of ribosomal proteins from archaebacteria.

G Schmid, A Böck
PMCID: PMC216043  PMID: 6167563

Abstract

Antisera were raised in rabbits against ribosomal proteins of Methanobacterium bryantii and used to analyze immunological relationships to ribosomes from other archaebacteria, from eubacteria, and from yeasts. Cross-reaction could be detected within the methanogens and with a member of the extreme halophiles; the degree of immunological similarity reflected the relationship delineated by 16S ribosomal ribonucleic acid oligonucleotide analysis (Fox et al., Science 209:457-463, 1980). With the methods and the anti-total-protein sera employed, there was no detectable cross-reaction with ribosomal proteins or ribosomes from Sulfolobus sp., eubacteria, or yeast.

Full text

PDF
282

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fox G. E., Stackebrandt E., Hespell R. B., Gibson J., Maniloff J., Dyer T. A., Wolfe R. S., Balch W. E., Tanner R. S., Magrum L. J. The phylogeny of prokaryotes. Science. 1980 Jul 25;209(4455):457–463. doi: 10.1126/science.6771870. [DOI] [PubMed] [Google Scholar]
  3. Geisser M., Tischendorf G. W., Stöffler G., Wittmann H. G. Immunological and electrophoretical comparison of ribosomal proteins from eight species belonging to Enterobacteriaceae. Mol Gen Genet. 1973 Dec 20;127(2):111–128. doi: 10.1007/BF00333660. [DOI] [PubMed] [Google Scholar]
  4. Hardy S. J., Kurland C. G., Voynow P., Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969 Jul;8(7):2897–2905. doi: 10.1021/bi00835a031. [DOI] [PubMed] [Google Scholar]
  5. Hennecke H., Böck A., Thomale J., Nass G. Threonyl-transfer ribonucleic acid synthetase from Escherichia coli: subunit structure and genetic analysis of the structural gene by means of a mutated enzyme and of a specialized transducing lambda bacteriophage. J Bacteriol. 1977 Sep;131(3):943–950. doi: 10.1128/jb.131.3.943-950.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaltschmidt E., Wittmann H. G. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Anal Biochem. 1970 Aug;36(2):401–412. doi: 10.1016/0003-2697(70)90376-3. [DOI] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Magrum L. J., Luehrsen K. R., Woese C. R. Are extreme halophiles actually "bacteria"? J Mol Evol. 1978 May 12;11(1):1–8. doi: 10.1007/BF01768019. [DOI] [PubMed] [Google Scholar]
  9. Matheson A. T., Yaguchi M., Balch W. E., Wolfe R. S. Sequence homologies in the N-terminal region of the ribosomal 'A' proteins from Methanobacterium Thermoautotrophicum and Halobacterium cutirubrum. Biochim Biophys Acta. 1980 Nov 20;626(1):162–169. doi: 10.1016/0005-2795(80)90207-x. [DOI] [PubMed] [Google Scholar]
  10. Stöffler G. Ribosomal proteins. II. A rapid micromethod for electrophoretic separation of Escherichia coli ribosomal protein on gelatinized cellulose acetate. Mol Gen Genet. 1967;100(4):374–377. doi: 10.1007/BF00334064. [DOI] [PubMed] [Google Scholar]
  11. Stöffler G., Wittmann H. G. Ribosomal proteins. XXV. Immunological studies on Escherichia coli ribosomal proteins. J Mol Biol. 1971 Dec 14;62(2):407–409. doi: 10.1016/0022-2836(71)90436-0. [DOI] [PubMed] [Google Scholar]
  12. Visentin L. P., Chow C., Matheson A. T., Yaguchi M., Rollin F. Halobacterium cutirubrum ribosomes. Properties of the ribosomal proteins and ribonucleic acid. Biochem J. 1972 Nov;130(1):103–110. doi: 10.1042/bj1300103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weiss R. L. Subunit cell wall of Sulfolobus acidocaldarius. J Bacteriol. 1974 Apr;118(1):275–284. doi: 10.1128/jb.118.1.275-284.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Zubke W., Stadler H., Ehrlich R., Stöffler G., Wittmann H. G., Apirion D. Improved electrophoretic and immunochemical techniques for the identification and characterization of mutant proteins, applied to ribosomal protein S8 in Escherichia coli mutants. Mol Gen Genet. 1977 Dec 30;158(2):129–139. doi: 10.1007/BF00268305. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES