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. 1981 Aug;147(2):297–304. doi: 10.1128/jb.147.2.297-304.1981

Identification of the tetracycline resistance promoter and repressor in transposon Tn10.

L V Wray Jr, R A Jorgensen, W S Reznikoff
PMCID: PMC216045  PMID: 6267006

Abstract

The structural and regulatory functions encoding tetracycline resistance in transposon Tn10 lie within a 2,700-base pair region. Using recombinant plasmids with different deoxyribonucleic acid sequences adjacent to a HincII site in this region, we located the promoter controlling the expression of tetracycline resistance. These various sequences conferred altered levels of tetracycline resistance. Plasmids containing deletions of a 695-base pair HincII fragment were constitutive and showed the loss of a 23,000-dalton tetracycline-inducible polypeptide, thus identifying the repressor and the location of its gene.

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Selected References

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