Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Aug;147(2):517–525. doi: 10.1128/jb.147.2.517-525.1981

Spontaneous mitotic recombination in mms8-1, an allele of the CDC9 gene of Saccharomyces cerevisiae.

B A Montelone, S Prakash, L Prakash
PMCID: PMC216072  PMID: 7021533

Abstract

The methyl methane sulfonate (MMS)-sensitive mutation mms8-1 increases the rate of spontaneous mitotic intragenic recombination at five heteroallelic loci on three chromosomes. Complementation, segregation, and mapping studies indicate that mms8-1 is allelic to cdc9, known to be defective in deoxyribonucleic acid ligase. Both mms8-1 and cdc9 mutants are lethal in combination with the recombination-defective mutant rad52-1. Genetic analysis of spontaneous red/white sectors in an ade2-1/ade2-1 ade5/+ mms8-1/mms8-1 strain shows nonreciprocal recombinational events involving long chromosome segments. We also observe greater than expected rates of simultaneous recombination at loci on different chromosomes in both wild-type and mms8-1 mutants.

Full text

PDF
517

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boram W. R., Roman H. Recombination in Saccharomyces cerevisiae: a DNA repair mutation associated with elevated mitotic gene conversion. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2828–2832. doi: 10.1073/pnas.73.8.2828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Esposito M. S. Evidence that spontaneous mitotic recombination occurs at the two-strand stage. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4436–4440. doi: 10.1073/pnas.75.9.4436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FOGEL S., HURST D. D. Coincidence relations between gene conversion and mitotic recombination in Saccharomyces. Genetics. 1963 Mar;48:321–328. doi: 10.1093/genetics/48.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fabre F. Induced intragenic recombination in yeast can occur during the G1 mitotic phase. Nature. 1978 Apr 27;272(5656):795–798. doi: 10.1038/272795a0. [DOI] [PubMed] [Google Scholar]
  5. Fogel S., Mortimer R., Lusnak K., Tavares F. Meiotic gene conversion: a signal of the basic recombination event in yeast. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 2):1325–1341. doi: 10.1101/sqb.1979.043.01.152. [DOI] [PubMed] [Google Scholar]
  6. Game J. C., Johnston L. H., von Borstel R. C. Enhanced mitotic recombination in a ligase-defective mutant of the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4589–4592. doi: 10.1073/pnas.76.9.4589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Game J. C., Zamb T. J., Braun R. J., Resnick M., Roth R. M. The Role of Radiation (rad) Genes in Meiotic Recombination in Yeast. Genetics. 1980 Jan;94(1):51–68. doi: 10.1093/genetics/94.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Golin J. E., Esposito M. S. Evidence for joint genic control of spontaneous mutation and genetic recombination during mitosis in Saccharomyces. Mol Gen Genet. 1977 Jan 18;150(2):127–135. doi: 10.1007/BF00695392. [DOI] [PubMed] [Google Scholar]
  9. Hartwell L. H., Culotti J., Pringle J. R., Reid B. J. Genetic control of the cell division cycle in yeast. Science. 1974 Jan 11;183(4120):46–51. doi: 10.1126/science.183.4120.46. [DOI] [PubMed] [Google Scholar]
  10. Hartwell L. H., Mortimer R. K., Culotti J., Culotti M. Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants. Genetics. 1973 Jun;74(2):267–286. doi: 10.1093/genetics/74.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hawthorne D. C., Leupold U. Suppressors in yeast. Curr Top Microbiol Immunol. 1974;64(0):1–47. doi: 10.1007/978-3-642-65848-8_1. [DOI] [PubMed] [Google Scholar]
  12. Johnston J. R. Genetic analysis of spontaneous half-sectored colonies of Saccharomyces cerevisiae. Genet Res. 1971 Oct;18(2):179–184. doi: 10.1017/s0016672300012581. [DOI] [PubMed] [Google Scholar]
  13. Johnston L. H., Nasmyth K. A. Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase. Nature. 1978 Aug 31;274(5674):891–893. doi: 10.1038/274891a0. [DOI] [PubMed] [Google Scholar]
  14. Johnston L. H. The DNA repair capability of cdc9, the Saccharomyces cerevisiae mutant defective in DNA ligase. Mol Gen Genet. 1979 Feb 16;170(1):89–92. doi: 10.1007/BF00268583. [DOI] [PubMed] [Google Scholar]
  15. Konrad E. B., Lehman I. R. A conditional lethal mutant of Escherichia coli K12 defective in the 5' leads to 3' exonuclease associated with DNA polymerase I. Proc Natl Acad Sci U S A. 1974 May;71(5):2048–2051. doi: 10.1073/pnas.71.5.2048. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Konrad E. B. Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications. J Bacteriol. 1977 Apr;130(1):167–172. doi: 10.1128/jb.130.1.167-172.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. MANNEY T. R., MORTIMER R. K. ALLELIC MAPPING IN YEAST BY X-RAY-INDUCED MITOTIC REVERSION. Science. 1964 Feb 7;143(3606):581–583. doi: 10.1126/science.143.3606.581. [DOI] [PubMed] [Google Scholar]
  18. Maloney D. H., Fogel S. Mitotic recombination in yeast: isolation and characterization of mutants with enhanced spontaneous mitotic gene conversion rates. Genetics. 1980 Apr;94(4):825–839. doi: 10.1093/genetics/94.4.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Marinus M. G., Morris N. R. Biological function for 6-methyladenine residues in the DNA of Escherichia coli K12. J Mol Biol. 1974 May 15;85(2):309–322. doi: 10.1016/0022-2836(74)90366-0. [DOI] [PubMed] [Google Scholar]
  20. Pauling C., Hamm L. Properties of a temperature-sensitive radiation-sensitive mutant of Escherichia coli. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1495–1502. doi: 10.1073/pnas.60.4.1495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pauling C., Hamm L. Properties of a temperature-sensitive, radiation-sensitive mutant of Escherichia coli. II. DNA replication. Proc Natl Acad Sci U S A. 1969 Dec;64(4):1195–1202. doi: 10.1073/pnas.64.4.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Prakash L., Prakash S. Isolation and characterization of MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics. 1977 May;86(1):33–55. doi: 10.1093/genetics/86.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Prakash S., Prakash L., Burke W., Montelone B. A. Effects of the RAD52 Gene on Recombination in SACCHAROMYCES CEREVISIAE. Genetics. 1980 Jan;94(1):31–50. doi: 10.1093/genetics/94.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Prakash S., Prakash L. Increased spontaneous mitotic segregation in MMS-sensitive mutants of Saccharomyces cerevisiae. Genetics. 1977 Oct;87(2):229–236. doi: 10.1093/genetics/87.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tye B. K., Nyman P. O., Lehman I. R., Hochhauser S., Weiss B. Transient accumulation of Okazaki fragments as a result of uracil incorporation into nascent DNA. Proc Natl Acad Sci U S A. 1977 Jan;74(1):154–157. doi: 10.1073/pnas.74.1.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wildenberg J. The relation of mitotic recombination to DNA replication in yeast pedigrees. Genetics. 1970 Oct;66(2):291–304. doi: 10.1093/genetics/66.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES