Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Aug;147(2):670–674. doi: 10.1128/jb.147.2.670-674.1981

Scanning electron probe x-ray microanalysis of elemental distributions in freeze-dried cryosections of Bacillus coagulans spores.

M Stewart, A P Somlyo, A V Somlyo, H Shuman, J A Lindsay, W G Murrell
PMCID: PMC216088  PMID: 7263617

Abstract

High-resolution scanning electron probe X-ray microanalysis had been employed to examine elemental distributions in freeze-dried cryosections of Bacillus coagulans spores. Calcium, manganese, and phosphorus were concentrated in the protoplast and the coat. Iron was found in the coat but not in the protoplast, whereas the silicon seen on the coat of other spore species was absent. Sulfur was present in the coat, but was distributed over a broader area than the other elements, which suggested that phosphorus and the metal ions were located in the outer coat layer.

Full text

PDF
670

Images in this article

672Image
on p.672
673Image
on p.673

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ando Y. [Properties of ionic forms of spores of a Clostridium perfringens]. Nihon Saikingaku Zasshi. 1976;31(6):713–717. [PubMed] [Google Scholar]
  2. Aronson A. I., Fitz-James P. Structure and morphogenesis of the bacterial spore coat. Bacteriol Rev. 1976 Jun;40(2):360–402. doi: 10.1128/br.40.2.360-402.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Franks F., Asquith M. H., Hammond C. C., Skaer H. B., Echlin P. Polymer cryoprotectants in the preservation of biological ultrastructure. I. Low temperature states of aqueous solutions of hydrophilic polymers. J Microsc. 1977 Aug;110(3):223–228. doi: 10.1111/j.1365-2818.1977.tb00034.x. [DOI] [PubMed] [Google Scholar]
  4. Nishihara T., Ichikawa T., Kondo M. Location of elements in ashed spores of Bacillus megaterium. Microbiol Immunol. 1980;24(6):495–506. doi: 10.1111/j.1348-0421.1980.tb02853.x. [DOI] [PubMed] [Google Scholar]
  5. OHYE D. F., MURRELL W. G. Formation and structure of the spore of Bacillus coagulans. J Cell Biol. 1962 Jul;14:111–123. doi: 10.1083/jcb.14.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Scherrer R., Gerhardt P. Location of calcium within Bacillus spores by electron probe x-ray microanalysis. J Bacteriol. 1972 Oct;112(1):559–568. doi: 10.1128/jb.112.1.559-568.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Shuman H., Somlyo A. V., Somlyo A. P. Quantitative electron probe microanalysis of biological thin sections: methods and validity. Ultramicroscopy. 1976 Sep-Oct;1(4):317–339. doi: 10.1016/0304-3991(76)90049-8. [DOI] [PubMed] [Google Scholar]
  8. Somlyo A. P., Somlyo A. V., Shuman H., Stewart M. Electron probe analysis of muscle and X-ray mapping of biological specimens with a field emission gun. Scan Electron Microsc. 1979;(2):711–722. [PubMed] [Google Scholar]
  9. Stewart M., Somlyo A. P., Somlyo A. V., Shuman H., Lindsay J. A., Murrell W. G. Distribution of calcium and other elements in cryosectioned Bacillus cereus T spores, determined by high-resolution scanning electron probe x-ray microanalysis. J Bacteriol. 1980 Jul;143(1):481–491. doi: 10.1128/jb.143.1.481-491.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. YOUNG I. E., JAMES P. C. Chemical and morphological studies of bacterial spore formation. IV. The development of spore refractility. J Cell Biol. 1962 Jan;12:115–133. doi: 10.1083/jcb.12.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES