Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Sep;147(3):744–751. doi: 10.1128/jb.147.3.744-751.1981

Use of gene fusions to study expression of cysB, the regulatory gene of the cysteine regulon.

G Jagura-Burdzy, D Hulanicka
PMCID: PMC216109  PMID: 6792186

Abstract

Strains of escherichia coli were constructed in which the lacZ gene is fused to cysB, the positive regulator gene of the cysteine regulon. The fusion strains were used to study the regulation of the cysB gene by assaying the fused lacZ gene product. The introduction of a cysB allele, either on a plasmid or on an episome to the fusion strains, resulted in the decrease of beta-galactosidase activity. This implies that the cysB gene expression is autoregulated by its own product. The direction of cysB gene transcription was determined to be clockwise.

Full text

PDF
744

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bassford P. J., Jr, Silhavy T. J., Beckwith J. R. Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm. J Bacteriol. 1979 Jul;139(1):19–31. doi: 10.1128/jb.139.1.19-31.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Becker M. A., Kredich N. M., Tomkins G. M. The purification and characterization of O-acetylserine sulfhydrylase-A from Salmonella typhimurium. J Biol Chem. 1969 May 10;244(9):2418–2427. [PubMed] [Google Scholar]
  4. Casadaban M. J., Cohen S. N. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4530–4533. doi: 10.1073/pnas.76.9.4530. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Casadaban M. J. Regulation of the regulatory gene for the arabinose pathway, araC. J Mol Biol. 1976 Jul 5;104(3):557–566. doi: 10.1016/0022-2836(76)90120-0. [DOI] [PubMed] [Google Scholar]
  6. Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J Mol Biol. 1976 Jul 5;104(3):541–555. doi: 10.1016/0022-2836(76)90119-4. [DOI] [PubMed] [Google Scholar]
  7. DEVITO P. C., DREYFUSS J. METABOLIC REGULATION OF ADENOSINE TRIPHOSPHATE SULFURYLASE IN YEAST. J Bacteriol. 1964 Nov;88:1341–1348. doi: 10.1128/jb.88.5.1341-1348.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Debarbouille M., Schwartz M. The use of gene fusions to study the expression of malT the positive regulator gene of the maltose regulon. J Mol Biol. 1979 Aug 15;132(3):521–534. doi: 10.1016/0022-2836(79)90273-0. [DOI] [PubMed] [Google Scholar]
  9. Dingman C. W., Fisher M. P., Kakefuda T. Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide gels. II. Biochemistry. 1972 Mar 28;11(7):1242–1250. doi: 10.1021/bi00757a020. [DOI] [PubMed] [Google Scholar]
  10. Eckhardt T. Use of argA-lac fusions to generate lambda argA-lac bacteriophages and to determine the direction of argA transcription in Escherichia coli. J Bacteriol. 1977 Oct;132(1):60–66. doi: 10.1128/jb.132.1.60-66.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Howe M. M. Prophage deletion mapping of bacteriophage Mu-1. Virology. 1973 Jul;54(1):93–101. doi: 10.1016/0042-6822(73)90118-9. [DOI] [PubMed] [Google Scholar]
  12. Howe M. M. Transduction by bacteriophage MU-1. Virology. 1973 Sep;55(1):103–117. doi: 10.1016/s0042-6822(73)81012-8. [DOI] [PubMed] [Google Scholar]
  13. Jagura G., Hulanicka D. Analysis of merodiploids of the cysB region in Salmonella typhimurium. Mol Gen Genet. 1978 Sep 20;165(1):31–38. doi: 10.1007/BF00270373. [DOI] [PubMed] [Google Scholar]
  14. Jones-Mortimer M. C. Positive control of sulphate reduction in Escherichia coli. The nature of the pleiotropic cysteineless mutants of E. coli K12. Biochem J. 1968 Dec;110(3):597–602. doi: 10.1042/bj1100597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kredich N. M. Regulation of L-cysteine biosynthesis in Salmonella typhimurium. I. Effects of growth of varying sulfur sources and O-acetyl-L-serine on gene expression. J Biol Chem. 1971 Jun 10;246(11):3474–3484. [PubMed] [Google Scholar]
  16. LURIA S. E., BURROUS J. W. Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957 Oct;74(4):461–476. doi: 10.1128/jb.74.4.461-476.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Reznikoff W. S., Michels C. A., Cooper T. G., Silverstone A. E., Magasanik B. Inhibition of lacZ gene translation initiation in trp-lac fusion strains. J Bacteriol. 1974 Mar;117(3):1231–1239. doi: 10.1128/jb.117.3.1231-1239.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smith J. M., Umbarger H. E. Characterization of fusions between the lac operon and the ilv gene cluster in Escherichia coli: ilvC-lac fusions. J Bacteriol. 1977 Dec;132(3):870–875. doi: 10.1128/jb.132.3.870-875.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tully M., Yudkin M. D. Fine-structure mapping and complementation analysis of the Escherichia coli cysB gene. J Bacteriol. 1977 Jul;131(1):49–56. doi: 10.1128/jb.131.1.49-56.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. VOGEL H. J., BONNER D. M. Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956 Jan;218(1):97–106. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES