Abstract
We report the characterization of an amino acid 2-aminoisobutyric acid was transported solely by the general amino acid permease and not by the neutral amino acid permease. Furthermore, this substrate was not metabolized after transport. The potential for a system-specific nonmetabolizable substrate as a tool in the analysis of amino acid transport and its regulation is discussed.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DeBusk B. G., DeBusk A. G. Molecular transport in Neurospora crassa. I. Biochemical properties of a phenylalanine permease. Biochim Biophys Acta. 1965 Jun 15;104(1):139–150. doi: 10.1016/0304-4165(65)90229-1. [DOI] [PubMed] [Google Scholar]
- DeBusk R. M., DeBusk A. G. Physiological and regulatory properties of the general amino acid transport system of Neurospora crassa. J Bacteriol. 1980 Jul;143(1):188–197. doi: 10.1128/jb.143.1.188-197.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kotyk A., Ríhová L. Transport of -aminoisobutyric acid in Saccharomyces cerevisiae. Biochim Biophys Acta. 1972 Nov 2;288(2):380–389. doi: 10.1016/0005-2736(72)90259-3. [DOI] [PubMed] [Google Scholar]
- Lester G. Genetic control of amino acid permeability in Neurospora crassa. J Bacteriol. 1966 Feb;91(2):677–684. doi: 10.1128/jb.91.2.677-684.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pall M. L. Amino acid transport in Neurospora crassa. I. Properties of two amino acid transport systems. Biochim Biophys Acta. 1969 Jan 28;173(1):113–127. doi: 10.1016/0005-2736(69)90042-x. [DOI] [PubMed] [Google Scholar]
- Rao E. Y., Rao T. K., Debusk A. G. Isolation and characterization of a mutant of Neurospora crassa deficient in general amino acid permease activity. Biochim Biophys Acta. 1975 Nov 17;413(1):45–51. doi: 10.1016/0005-2736(75)90057-7. [DOI] [PubMed] [Google Scholar]
- Stadler D. R. Genetic control of the uptake of amino acids in Neurospora. Genetics. 1966 Aug;54(2):677–685. doi: 10.1093/genetics/54.2.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thwaites W. M., Pendyala L. Regulation of amino acid assimilation in a strain of Neurospora crassa lacking basic amino acid transport activity. Biochim Biophys Acta. 1969 Dec 30;192(3):455–461. doi: 10.1016/0304-4165(69)90394-8. [DOI] [PubMed] [Google Scholar]
- Tisdale J. H., DeBusk A. G. Developmental regulation of amino acid transport in Neurospora crassa. J Bacteriol. 1970 Nov;104(2):689–697. doi: 10.1128/jb.104.2.689-697.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wolfinbarger L., Jr, Debusk A. G. Molecular transport. I. In vivo studies of transport mutants of Neurospora crassa with altered amino acid competition patterns. Arch Biochem Biophys. 1971 Jun;144(2):503–511. doi: 10.1016/0003-9861(71)90355-9. [DOI] [PubMed] [Google Scholar]