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. 1981 Sep;147(3):1015–1020. doi: 10.1128/jb.147.3.1015-1020.1981

Plasmid ColVBtrp maintenance in Erwinia carotovora.

N N Schukin
PMCID: PMC216141  PMID: 6944305

Abstract

Plasmid ColVBtrp maintenance in Erwinia carotovora cells was followed by measuring kinetics of elimination of plasmid genetic markers and loss of plasmid deoxyribonucleic acid. An E. carotovora mutant stably carrying plasmid ColVBtrp was isolated. Besides stable plasmid maintenance, the mutant showed altered sensitivity to male-specific phage MS2, sensitivity to drugs, and colony morphology.

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Selected References

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  1. Bazzicalupo P., Tocchini-Valentini G. P. Curing of an Escherichia coli episome by rifampicin (acridine orange-F + -F - -Hfr-lac). Proc Natl Acad Sci U S A. 1972 Feb;69(2):298–300. doi: 10.1073/pnas.69.2.298. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cohen S. N. Transposable genetic elements and plasmid evolution. Nature. 1976 Oct 28;263(5580):731–738. doi: 10.1038/263731a0. [DOI] [PubMed] [Google Scholar]
  3. DiJoseph C. G. The thermosensitive lesion in the replication of the drug resistance factor, Rts1. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2515–2519. doi: 10.1073/pnas.71.6.2515. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Falkow S., Guerry P., Hedges R. W., Datta N. Polynucleotide sequence relationships among plasmids of the I compatibility complex. J Gen Microbiol. 1974 Nov;85(1):65–76. doi: 10.1099/00221287-85-1-65. [DOI] [PubMed] [Google Scholar]
  5. Freifelder D., Folkmanis A., Kirschner I. Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles. J Bacteriol. 1971 Mar;105(3):722–727. doi: 10.1128/jb.105.3.722-727.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hasunuma K., Sekiguchi M. Effect of dna mutations on the replication of plasmid pSC101 in Escherichia coli K-12. J Bacteriol. 1979 Mar;137(3):1095–1099. doi: 10.1128/jb.137.3.1095-1099.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hickson F. T., Roth T. F., Helinski D. R. Circular DNA forms of a bacterial sex factor. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1731–1738. doi: 10.1073/pnas.58.4.1731. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hirota Y. THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1960 Jan;46(1):57–64. doi: 10.1073/pnas.46.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jacob F., Ryter A., Cuzin F. On the association between DNA and membrane in bacteria. Proc R Soc Lond B Biol Sci. 1966 Mar 22;164(995):267–278. doi: 10.1098/rspb.1966.0029. [DOI] [PubMed] [Google Scholar]
  10. Jaffé-Brachet A., D'Ari R. Maintenance of bacteriophage P1 plasmid. J Virol. 1977 Sep;23(3):476–482. doi: 10.1128/jvi.23.3.476-482.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kamio Y., Ishihara M., Terawaki Y. Alteration of flagella by a temperature sensitive R plasmid Rtsl in Escherichia coli K-12. Biochem Biophys Res Commun. 1978 Nov 14;85(1):301–308. doi: 10.1016/s0006-291x(78)80043-6. [DOI] [PubMed] [Google Scholar]
  12. Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
  13. Koyama A. H., Wada C., Nagata T., Yura T. Indirect selection for plasmid mutants: isolation of ColVBtrp mutants defective in self-maintenance in Escherichia coli. J Bacteriol. 1975 Apr;122(1):73–79. doi: 10.1128/jb.122.1.73-79.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Koyama A. H., Yura T. Plasmid mutations affecting self-maintenance and host growth in Escherichia coli. J Bacteriol. 1975 Apr;122(1):80–88. doi: 10.1128/jb.122.1.80-88.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Levy S. B., McMurry L., Palmer E. R factor proteins synthesized in Escherichia coli minicells: membrane-associated R factor proteins. J Bacteriol. 1974 Dec;120(3):1464–1471. doi: 10.1128/jb.120.3.1464-1471.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Levy S. B. R factor proteins synthesized in Escherichia coli minicells: incorporation studies with different R factors and detection of deoxyribonucleic acid-binding proteins. J Bacteriol. 1974 Dec;120(3):1451–1463. doi: 10.1128/jb.120.3.1451-1463.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. NOVICK R. P., RICHMOND M. H. NATURE AND INTERACTIONS OF THE GENETIC ELEMENTS GOVERNING PENICILLINASE SYNTHESIS IN STAPHYLOCOCCUS AUREUS. J Bacteriol. 1965 Aug;90:467–480. doi: 10.1128/jb.90.2.467-480.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nazar K. Usuwanie plazmidu penicylinazy z komórek Staphylococcus epidermidis wybranymi czynnikami mutagennymi. Med Dosw Mikrobiol. 1977;29(4):229–233. [PubMed] [Google Scholar]
  19. Novick R. P., Hoppensteadt F. C. On plasmid incompatibility. Plasmid. 1978 Sep;1(4):421–434. doi: 10.1016/0147-619x(78)90001-x. [DOI] [PubMed] [Google Scholar]
  20. Novick R., Sanchez-Rivas C., Gruss A., Edelman I. Involvement of the cell envelope in plasmid maintenance: plasmid curing during the regeneration of protoplasts. Plasmid. 1980 May;3(3):348–358. doi: 10.1016/0147-619x(80)90048-7. [DOI] [PubMed] [Google Scholar]
  21. Rodriguez Lemoine V., Rowbury R. J. Instability of the Flac+ factor in a dnaC mutant of Salmonella typhimurium. J Gen Microbiol. 1975 Oct;90(2):360–363. doi: 10.1099/00221287-90-2-360. [DOI] [PubMed] [Google Scholar]
  22. Schukin N. N., Avdienko I. D., Goldfarb D. M. Plasmid pEA566 from Erwinia aroideae. Plasmid. 1980 Sep;4(2):228–230. doi: 10.1016/0147-619x(80)90012-8. [DOI] [PubMed] [Google Scholar]
  23. Smith H. W. Transmissible pathogenic characteristics of invasive strains of Escherichia coli. J Am Vet Med Assoc. 1978 Sep 1;173(5 Pt 2):601–607. [PubMed] [Google Scholar]
  24. Taylor D. E., Levine J. G. Characterization of a plasmid mutation affecting maintenance, transfer and elimination by novobiocin. Mol Gen Genet. 1979 Jul 13;174(2):127–133. doi: 10.1007/BF00268350. [DOI] [PubMed] [Google Scholar]
  25. Terawaki Y., Kakizawa Y., Takayasu H., Yoshikawa M. Temperature sensitivity of cell growth in Escherichia coli associated with the temperature sensitive R(KM) factor. Nature. 1968 Jul 20;219(5151):284–285. doi: 10.1038/219284a0. [DOI] [PubMed] [Google Scholar]
  26. Terawaki Y., Kobayashi Y. Further characterization of the R plasmid Rts1 and its mutant pTW2: replication and incompatibility of the plasmid. J Bacteriol. 1978 Aug;135(2):300–306. doi: 10.1128/jb.135.2.300-306.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Terawaki Y., Takayasu H., Akiba T. Thermosensitive replication of a kanamycin resistance factor. J Bacteriol. 1967 Sep;94(3):687–690. doi: 10.1128/jb.94.3.687-690.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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