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. 1981 Oct;148(1):58–63. doi: 10.1128/jb.148.1.58-63.1981

Variation in Escherichia coli buoyant density measured in Percoll gradients.

C L Woldringh, J S Binnerts, A Mans
PMCID: PMC216166  PMID: 6270065

Abstract

Escherichia coli B/r cells, centrifuged to equilibrium in either self-generating or preformed gradients of Percoll, banded at an average density of 1.080 to 1.100 g/ml, depending on their growth rate and the temperature of centrifugation. Cells cultured in gradient material (70% Percoll) exhibited the same average density. At the various growth rates examined, the density of the individual cells in a steady-state population varied by less than 1% of the mean in E. coli strains B/r and B, as well as K-12. Electron microscope analysis of the density fractions of both fast- and slow-growing E. coli B/r populations suggested a small increase in density during cell constriction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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