Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Oct;148(1):241–247. doi: 10.1128/jb.148.1.241-247.1981

Proline transport in Saccharomyces cerevisiae.

P F Lasko, M C Brandriss
PMCID: PMC216186  PMID: 7026531

Abstract

The yeast Saccharomyces cerevisiae is capable of utilizing proline as the sole source of nitrogen. Mutants of S. cerevisiae with defective proline transport were isolated by selecting for resistance to either of the toxic proline analogs L-azetidine-2-carboxylate or 3,4-dehydro-DL-proline. Strains carrying the put4 mutation are defective in the high-affinity proline transport system. These mutants could still grow when given high concentrations of proline, due to the operation of low-affinity systems whose existence as confirmed by kinetic studies. Both systems were repressed by ammonium ions, and either was induce by proline. Low-affinity transport was inhibited by histidine, so put4 mutants were unable to grow on a medium containing high concentrations of proline to which histidine has been added.

Full text

PDF
241

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arst H. N., Jr, MacDonald D. W. Reduced expression of a distal gene of the prn gene cluster in deletion mutants of Aspergillus nidulans: genetic evidence for a dicistronic messenger in an eukaryote. Mol Gen Genet. 1978 Jul 6;163(1):17–22. doi: 10.1007/BF00268959. [DOI] [PubMed] [Google Scholar]
  2. Brandriss M. C., Magasanik B. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: enzyme induction by proline. J Bacteriol. 1979 Nov;140(2):498–503. doi: 10.1128/jb.140.2.498-503.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brandriss M. C., Magasanik B. Genetics and physiology of proline utilization in Saccharomyces cerevisiae: mutation causing constitutive enzyme expression. J Bacteriol. 1979 Nov;140(2):504–507. doi: 10.1128/jb.140.2.504-507.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brandriss M. C., Magasanik B. Subcellular compartmentation in control of converging pathways for proline and arginine metabolism in Saccharomyces cerevisiae. J Bacteriol. 1981 Mar;145(3):1359–1364. doi: 10.1128/jb.145.3.1359-1364.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Crabeel M., Grenson M. Regulation of histidine uptake by specific feedback inhibition of two histidine permeases in Saccharomyces cerevisiae. Eur J Biochem. 1970 May 1;14(1):197–204. doi: 10.1111/j.1432-1033.1970.tb00278.x. [DOI] [PubMed] [Google Scholar]
  6. Eavenson E., Christensen H. N. Transport systems for neutral amino acids in the pigeon erythrocyte. J Biol Chem. 1967 Nov 25;242(22):5386–5396. [PubMed] [Google Scholar]
  7. Grenson M., Hennaut C. Mutation affecting activity of several distinct amino acid transport systems in Saccharomyces cerevisiae. J Bacteriol. 1971 Feb;105(2):477–482. doi: 10.1128/jb.105.2.477-482.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grenson M., Hou C., Crabeel M. Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease. J Bacteriol. 1970 Sep;103(3):770–777. doi: 10.1128/jb.103.3.770-777.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hampton J. R. Lysine uptake in cultured Trypanosoma cruzi: interactions of competitive inhibitors. J Protozool. 1970 Nov;17(4):597–600. doi: 10.1111/j.1550-7408.1970.tb04734.x. [DOI] [PubMed] [Google Scholar]
  10. Kay W. W., Gronlund A. F. Proline transport by Pseudomonas aeruginosa. Biochim Biophys Acta. 1969;193(2):444–455. doi: 10.1016/0005-2736(69)90203-x. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Law S. S., Mukkada A. J. Transport of L-proline and its regulation in Leishmania tropica promastigotes. J Protozool. 1979 May;26(2):295–301. doi: 10.1111/j.1550-7408.1979.tb02784.x. [DOI] [PubMed] [Google Scholar]
  13. Magaña-Schwencke N., Schwencke J. A proline transport system in Saccharomyces chevalieri. Biochim Biophys Acta. 1969 Mar 11;173(2):313–323. doi: 10.1016/0005-2736(69)90114-x. [DOI] [PubMed] [Google Scholar]
  14. Menzel R., Roth J. Identification and mapping of a second proline permease Salmonella typhimurium. J Bacteriol. 1980 Mar;141(3):1064–1070. doi: 10.1128/jb.141.3.1064-1070.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morikawa A., Suzuki H., Anraku Y. Transport of sugars and amino acids in bacteria. 8. Properties and regulation of the active transport reaction of proline in Escherichia coli. J Biochem. 1974 Feb;75(2):229–241. doi: 10.1093/oxfordjournals.jbchem.a130390. [DOI] [PubMed] [Google Scholar]
  16. OXENDER D. L., CHRISTENSEN H. N. DISTINCT MEDIATING SYSTEMS FOR THE TRANSPORT OF NEUTRAL AMINO ACIDS BY THE EHRLICH CELL. J Biol Chem. 1963 Nov;238:3686–3699. [PubMed] [Google Scholar]
  17. Ratzkin B., Grabnar M., Roth J. Regulation of the major proline permease gene of Salmonella typhimurium. J Bacteriol. 1978 Feb;133(2):737–743. doi: 10.1128/jb.133.2.737-743.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schwencke J., Magaña-Schwencke N. Derepression of a proline transport system in Saccharomyces chevalieri by nitrogen starvation. Biochim Biophys Acta. 1969 Mar 11;173(2):302–312. doi: 10.1016/0005-2736(69)90113-8. [DOI] [PubMed] [Google Scholar]
  19. Stewart P. R. Analytical methods for yeasts. Methods Cell Biol. 1975;12:111–147. doi: 10.1016/s0091-679x(08)60955-3. [DOI] [PubMed] [Google Scholar]
  20. Surdin Y., Sly W., Sire J., Bordes A. M., Robichon-Szulmajster H. Propriétés et contrôle génétique du système d'accumulation des acides aminés chez Saccharomyces cerevisiae. Biochim Biophys Acta. 1965 Oct 18;107(3):546–566. [PubMed] [Google Scholar]
  21. Thomas E. L., Christensen H. N. Nature of the cosubstrate action of Na+ and neutral amino acids in a transport system. J Biol Chem. 1971 Mar 25;246(6):1682–1688. [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES