Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1981 Oct;148(1):361–364. doi: 10.1128/jb.148.1.361-364.1981

Regulatory isozymes of 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase in the cyanobacterium Anabaena sp. strain ATCC 29151.

G C Hall, R A Jensen
PMCID: PMC216200  PMID: 6116697

Abstract

Two regulatory isozymes of 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase from the cyanobacterium Anabaena sp. strain ATCC 29151 were separated via gel filtration. One isozyme (Mr = 205,000) was sensitive to phenylalanine inhibition, and the other (Mr = 60,000) was sensitive to tyrosine inhibition. The tyrosine-sensitive isozyme from wild-type cells was unstable to gel filtration, whereas the corresponding isozyme from an analog-resistant mutant was insensitive to tyrosine inhibition and was stable.

Full text

PDF
361

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem J. 1964 May;91(2):222–233. doi: 10.1042/bj0910222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  3. Doy C. H., Brown K. D. Control of aromatic biosynthesis: the multiplicity of 7-phospho-2-oxo-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating) in Escherichia coli W. Biochim Biophys Acta. 1965 Jul 8;104(2):377–389. doi: 10.1016/0304-4165(65)90343-0. [DOI] [PubMed] [Google Scholar]
  4. Hall G. C., Jensen R. A. Enzymological basis for growth inhibition by L-phenylalanine in the cyanobacterium Synechocystis sp. 29108. J Bacteriol. 1980 Dec;144(3):1034–1042. doi: 10.1128/jb.144.3.1034-1042.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hall G., Flick M. B., Jensen R. A. Approach to recognition of regulatory mutants of cyanobacteria. J Bacteriol. 1980 Aug;143(2):981–988. doi: 10.1128/jb.143.2.981-988.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Holtzclaw W. D., Chapman L. F., Gowans C. S. Some regulatory properties of phospho-2-keto-3-deoxyheptonate aldolase from the blue-green alga Anacystis nidulans. Biochim Biophys Acta. 1972 May 12;268(2):562–572. doi: 10.1016/0005-2744(72)90353-1. [DOI] [PubMed] [Google Scholar]
  7. Jensen R. A. Enzyme recruitment in evolution of new function. Annu Rev Microbiol. 1976;30:409–425. doi: 10.1146/annurev.mi.30.100176.002205. [DOI] [PubMed] [Google Scholar]
  8. Jensen R. A., Nasser D. S. Comparative regulation of isoenzymic 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetases in microorganisms. J Bacteriol. 1968 Jan;95(1):188–196. doi: 10.1128/jb.95.1.188-196.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jensen R. A., Nasser D. S., Nester E. W. Comparative control of a branch-point enzyme in microorganisms. J Bacteriol. 1967 Nov;94(5):1582–1593. doi: 10.1128/jb.94.5.1582-1593.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McCandliss R. J., Poling M. D., Herrmann K. M. 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification and molecular characterization of the phenylalanine-sensitive isoenzyme from Escherichia coli. J Biol Chem. 1978 Jun 25;253(12):4259–4265. [PubMed] [Google Scholar]
  11. SMITH L. C., RAVEL J. M., LAX S. R., SHIVE W. The control of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthesis by phenylalanine and tyrosine. J Biol Chem. 1962 Nov;237:3566–3570. [PubMed] [Google Scholar]
  12. SRINIVASAN P. R., SPRINSON D. B. 2-Keto-3-deoxy-D-arabo-heptonic acid 7-phosphate synthetase. J Biol Chem. 1959 Apr;234(4):716–722. [PubMed] [Google Scholar]
  13. Schoner R., Herrmann K. M. 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification, properties, and kinetics of the tyrosine-sensitive isoenzyme from Escherichia coli. J Biol Chem. 1976 Sep 25;251(18):5440–5447. [PubMed] [Google Scholar]
  14. Wallace B. J., Pittard J. Chromatography of 3-deoxy-D-arabinoheptulosonic acid-7-phosphate synthetase (trp) on diethylaminoethyl cellulose: a correction. J Bacteriol. 1967 Oct;94(4):1279–1280. doi: 10.1128/jb.94.4.1279-1280.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wallace B. J., Pittard J. Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli. J Bacteriol. 1967 Jan;93(1):237–244. doi: 10.1128/jb.93.1.237-244.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES