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. 1981 Oct;148(1):386–390. doi: 10.1128/jb.148.1.386-390.1981

Overproduction of the Escherichia coli recA protein without stimulation of its proteolytic activity.

B E Uhlin, A J Clark
PMCID: PMC216207  PMID: 6457028

Abstract

Plasmid pBEU14, which carries the Escherichia coli recA+ gene and which can be amplified by manipulation of growth temperature, was constructed. When pBEU14 deoxyribonucleic acid was amplified, a high rate of synthesis and accumulation of recA protein resulted. Amplification of the recA gene and protein did not cause induction of prophage lambda, indicating that the proteolytic activity of the recA protein was not stimulated.

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Selected References

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